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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 05 NOV 2020 - 27 JAN 2021
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 021
- Report date:
- 2021
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: “Testing Guidelines for Studies of Chemicals, Chapter 4 Degradation and Accumulation test field, Section 3 Biodegradation Study (Ready Biodegradability): Modified MITI(I)”
- Version / remarks:
- National Institute of Environmental Research Notification No. 2020-28 (Aug. 19, 2020)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Version / remarks:
- adopted on 17th July 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- bis(2,2,6,6-tetramethyl-1-(1,3-benzothiazol-2-ylsulfanyl)piperidin-4-yl)carbonate
- Cas Number:
- 2311845-49-3
- Molecular formula:
- C33H42N4O3S4
- IUPAC Name:
- bis(2,2,6,6-tetramethyl-1-(1,3-benzothiazol-2-ylsulfanyl)piperidin-4-yl)carbonate
- Test material form:
- solid: particulate/powder
Constituent 1
Study design
- Oxygen conditions:
- anaerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Remarks:
- Fresh samples collected from different sites in Korea were incubated for over one month and the activated sludge greater than 10^7 cells/mL was used in this test.
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Inoculum used in this study was collected from 11 sites in Korea including 5 rivers (Gangjeong, Mulgeum, Gwangju, Daejeon, Paldang), 4 domestic sewage treatment plants (Dalseo, Gumi, Busan, Jungrang) and 2 industrial wastewater treatment plants (Dalseo, Yeosu). Annual collection at 4 times (Mar, Jun, Sep and Dec)
- Storage conditions: Fresh samples collected from these sites were incubated for over one month, condition of the inoculum: dissolved oxygen 8.14 mg/L, pH 7.23 and temperature 25.1 °C
- Preparation of inoculum for exposure: Six bottles were prepared for exposure, mixing the activiated sludge with dionizied water, basal culture medium, nitrification inhibitor for treatment with the test item or control substance or control bottles as appropriate.
- Pretreatment: not specified
- Concentration of sludge: inoculum was treated at a concentration of about 10^7-10^8 cells/L - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Composition of stock solutions for basal culture medium were prepared as follows. Dissolve A, B, C and D solutions in deionized water at the ratio of 0.3 %.
Solution A : Dipotassium hydrogen orthophosphate 2.175 g, Potassium dihydrogen rthophosphate 0.850 g, Disodium hydrogen orthophosphate dodecahydrate 4.460 g, Ammonium chloride 0.170 g
Dissolve in deionized water and make up to 100 mL. The pH value of the solution is adjusted to 7.2.
Solution B : Magnesium sulfate heptahydrate 2.250 g, Dissolve in deionized water and make up to 100 mL.
Solution C : Calcium chloride anhydrous 2.750 g, Dissolve in deionized water and make up to 100 mL.
Solution D : Iron(III) chloride hexahydrate 0.025 g, Dissolve in deionized water and make up to 100 mL. The pH value of the solution is adjusted to 7.2.
- Test temperature: 24.5 - 25.7 °C
- pH: 7.1 - 7.2 (acceptable range: 6.0 - 8.5)
- Continuous darkness: yes
Other: The biodegradation test was conducted according to the SOP of “Biodegradation test” and the oxygen uptake was measured by the BOD meter equipped with 6 bottles. Reference compound, Sodium benzoate, was used to check the microbial activity of treated inoculums. Test substance and reference compound were treated to be about 100 mg/L(w/v) (16.05 mg and 16.02 mg, respectively) after correcting for purity.
TEST SYSTEM
- Culturing apparatus: Incubator: JEIO TECH Co., Ltd., IL-21, Korea
- Number of culture flasks/concentration: 3 bottles were treated with test item, and 3 bottles served as controls
- Measuring equipment: BOD meter: WTW, OxiTop® Control 6, Germany
SAMPLING
- Sampling frequency: The test was performed in the incubator to maintain a constant temperature and the oxygen uptake was checked once a day during test period.
- Sample storage before analysis: no storage indicated
CONTROL AND BLANK SYSTEM
- Inoculum blank: Activated sludge 5.1 mL + basal culture medium 1.92 mL + deionized water 152.82 mL + Nitrification inhibitor (C4H8N2S) 0.16 mL
- Abiotic sterile control: Test substance (16.05 mg) + deionized water 160 mL
- Toxicity control: no
- Other: Activity control: Sodium benzoate (16.02 mg) + Activated sludge 5.1 mL + basal culture medium 1.92 mL + deionized water 152.82 mL + Nitrification inhibitor (C4H8N2S) 0.16 mL
Reference substance
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- Sodium benzoate
Results and discussion
- Test performance:
- There was no environmental condition which might have affected the reliability of the test results and the test temperature was maintained at 24.5 - 25.7(25.1±0.2) °C during the test by monitoring of datalogger.
% Degradation
- Parameter:
- % degradation (test mat. analysis)
- Remarks:
- residual amount of test substance determined by HPLC-PDA analysis
- Value:
- 4.65
- Sampling time:
- 28 d
- Details on results:
- BOD concentrations of the inoculum blank (bottle 6) for 28 days were 0.0 - 45.0 mg O2/L.
These values were within the acceptable range (less than 60 mg O2/L for inoculums blank) specified by the OECD guideline.
The change of BOD concentrations for the test bottles was measured by BOD meter for 28 days. The difference of replicate value for the treatments (bottle 2, 3 and 4) at the end of the test was 4.5 % which was less than 20 % specified by the OECD guideline.
The pH values at the end of BOD measurement were 7.21, 7.16 and 7.16 for test bottle 2, 3 and 4, respectively. These values were within the acceptable range (6.0 - 8.5 for pH) specified by the OECD guideline.
The percentage biodegradation of test substance based on BOD measurement were calculated to be 0.0 % after 28 days.
The percentage biodegradations based on the residual amount of test substance by HPLC-PDA analysis was calculated to be 4.65 %.
BOD5 / COD results
- Results with reference substance:
- The percentage biodegradations of the reference compound (Sodium benzoate) were 76.0 % after 7 days and 77.5 % after 14 days and were within the acceptable range (greater than 40 % after 7 days and 65 % after 14 days) specified by the OECD guideline.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test substance could not be concluded as readily degradable by microorganisms in the aquatic environment. The average percentage biodegradation of the test substance by BOD measurement after 28 days was calculated to be 0.0 % which was less than pass level of biodegradability (60%) specified by the OECD Guideline. The percentage biodegradation based on the residual amount of test substance by HPLC-PDA analysis was calculated to be 4.65 %.
- Executive summary:
The biodegradation in water, screening test was performed according to OECD Guideline 301C under GLP compliance. Inoculum used in this study was collected from 11 sites in Korea including 5 rivers (Gangjeong, Mulgeum, Gwangju, Daejeon, Paldang), 4 domestic sewage treatment plants (Dalseo, Gumi, Busan, Jungrang) and 2 industrial wastewater treatment plants(Dalseo, Yeosu). The biodegradation test was conducted according to the SOP of “Biodegradation test” and the oxygen uptake was measured by the BOD meter equipped with 6 bottles. Reference compound, Sodium benzoate, was used to check the microbial activity of treated inoculums.
The test substance could not be concluded as readily degradable by microorganisms in the aquatic environment. The average percentage biodegradation of the test substance by BOD measurement after 28 days was calculated to be 0.0 % which was less than pass level of biodegradability (60%) specified by the OECD Guideline.
The percentage biodegradations based on the residual amount of test substance by HPLC-PDA analysis was calculated to be 4.65 %.
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