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EC number: 953-797-0 | CAS number: 210300-09-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in soil
Administrative data
- Endpoint:
- biodegradation in soil: simulation testing
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 307 (Aerobic and Anaerobic Transformation in Soil)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.4100 (Aerobic Soil Metabolism)
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- laboratory
Test material
- Reference substance name:
- 3-hydroxy-4-methoxypyridine-2-carboxylic acid
- Cas Number:
- 210300-09-7
- Molecular formula:
- C7H7NO4
- IUPAC Name:
- 3-hydroxy-4-methoxypyridine-2-carboxylic acid
Constituent 1
- Specific details on test material used for the study:
- Substance name: X696476
Lot #: XS9-109113-80
Purity: 100% - Radiolabelling:
- no
Study design
- Oxygen conditions:
- aerobic
- Soil classification:
- other: USDA Textural Class (hydrometer method)
Soil propertiesopen allclose all
- Soil no.:
- #1
- Soil type:
- clay loam
- % Clay:
- 29
- % Silt:
- 38
- % Sand:
- 33
- % Org. C:
- 3.7
- pH:
- 6.5
- CEC:
- 11.2 meq/100 g soil d.w.
- Bulk density (g/cm³):
- 1
- % Moisture content:
- 25.3
- Soil no.:
- #2
- Soil type:
- loam
- % Clay:
- 25
- % Silt:
- 50
- % Sand:
- 25
- % Org. C:
- 4.6
- pH:
- 6
- CEC:
- 16.2 meq/100 g soil d.w.
- Bulk density (g/cm³):
- 0.91
- % Moisture content:
- 41.9
- Soil no.:
- #3
- Soil type:
- clay loam
- % Clay:
- 39
- % Silt:
- 36
- % Sand:
- 25
- % Org. C:
- 4
- pH:
- 7.5
- CEC:
- 27 meq/100 g soil d.w.
- Bulk density (g/cm³):
- 1.1
- % Moisture content:
- 23.6
- Soil no.:
- #4
- Soil type:
- clay
- % Clay:
- 47
- % Silt:
- 22
- % Sand:
- 31
- % Org. C:
- 1.7
- pH:
- 7.9
- CEC:
- 11.8 meq/100 g soil d.w.
- Bulk density (g/cm³):
- 1.13
- % Moisture content:
- 16.3
Duration of test (contact time)open allclose all
- Soil No.:
- #1
- Duration:
- 120 d
- Soil No.:
- #2
- Duration:
- 120 d
- Soil No.:
- #3
- Duration:
- 120 d
- Soil No.:
- #4
- Duration:
- 120 d
Initial test substance concentrationopen allclose all
- Soil No.:
- #1
- Initial conc.:
- 0.25 mg/kg soil d.w.
- Based on:
- test mat.
- Soil No.:
- #2
- Initial conc.:
- 0.25 mg/kg soil d.w.
- Based on:
- test mat.
- Soil No.:
- #3
- Initial conc.:
- 0.25 mg/kg soil d.w.
- Based on:
- test mat.
- Soil No.:
- #4
- Initial conc.:
- 0.25 mg/kg soil d.w.
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- test mat. analysis
Experimental conditionsopen allclose all
- Soil No.:
- #1
- Temp.:
- 20 ± 2°C
- Microbial biomass:
- Initial: 492 μg C/g soil
Final: 274 μg C/g soil
- Soil No.:
- #2
- Temp.:
- 20 ± 2°C
- Microbial biomass:
- Initial: 551 μg C/g soil
Final: 403 μg C/g soil
- Soil No.:
- #3
- Temp.:
- 20 ± 2°C
- Microbial biomass:
- Initial: 853 μg C/g soil
Final: 710 μg C/g soil
- Soil No.:
- #4
- Temp.:
- 20 ± 2°C
- Microbial biomass:
- Initial: 416 μg C/g soil
Final: 282 μg C/g soil
Results and discussion
Half-life / dissipation time of parent compoundopen allclose all
- Key result
- Soil No.:
- #1
- DT50:
- > 1 000 d
- Type:
- other: SFO
- Temp.:
- 20 °C
- Remarks on result:
- other: RefSol (clay loam)
- Key result
- Soil No.:
- #2
- DT50:
- > 1 000 d
- Type:
- other: SFO
- Temp.:
- 20 °C
- Remarks on result:
- other: Chelmorton (loam)
- Key result
- Soil No.:
- #3
- DT50:
- > 1 000 d
- Type:
- other: SFO
- Temp.:
- 20 °C
- Remarks on result:
- other: Woodside Farm (clay loam)
- Key result
- Soil No.:
- #4
- DT50:
- > 1 000 d
- Type:
- other: SFO
- Temp.:
- 20 °C
- Remarks on result:
- other: Hareby (clay)
Applicant's summary and conclusion
- Conclusions:
- There was no apparent degradation of the test substance with the DT50 being calculated to be greater than 1000 days in all four soils. It can be concluded that the test substance may only degrade slowly in the environment.
- Executive summary:
The rate of degradation of the test substance has been investigated under aerobic conditions at 20 ± 2°C in four soils in the dark. The soils were RefSol (Germany, clay loam) Chelmorton (UK, loam), Woodside Farm (UK, clay loam) and Hareby (UK, clay).
The study was conducted to fulfil the requirements of Commission Regulation (EU) No 283/2013 of 01 March 2013 setting out the data requirements for active substances, in accordance with Regulation (EC) No 1107/2009 of the European Parliament and of the Council concerning the placing of plant protection products on the market. The experiments were conducted in accordance with OECD 307, Aerobic and Anaerobic Transformation in Soil (April 2002) and OPPTS 835.4100 Aerobic Soil Metabolism (October 2008).
The study was conducted in accordance with the OECD Principles of Good Laboratory Practice, based on Section II of Annex I to the European Parliament and Council Directive 2004/10/EC and embodied in the UK Good Laboratory Practice Regulations 1999 (Statutory Instrument No. 3106) as amended by the UK Good Laboratory Practice (Codification Amendments Etc.) regulations 2004 (Statutory Instrument No. 994), and as accepted by Regulatory Authorities throughout the European Community, the United States of America and Japan.
The soils, sieved to 2 mm, were dispensed [50g oven-dried equivalent (ode)] into individual flasks, the water content of the soil was adjusted to 50% of the maximum moisture holding capacity and the samples were incubated at 20 ± 2°C. Each flask was treated at a nominal application rate of 17.5 μg of the test substance per 50 g of oven dried equivalent (ode) soil, this being equivalent to 0.35 mg/kg or 130 g/ha.
Duplicate flasks were removed after incubation for 0, 2, 6, 14, 30, 63, 90 and 120 days. Each soil sample was extracted using accelerated solvent extraction with a series of solvents and conditions and, following solid phase clean up and concentration, the test substance present in the extracts was quantified by LC-MS/MS.
At each time-point three 50g (ode) samples of each soil were prepared from a quantity of untreated soil designated for the purpose of method efficiency determinations. Duplicate samples were fortified at a level approximately equivalent to the expected concentration of the test substance in the samples under analysis whilst a single control sample was left untreated. These samples were then extracted and analysed alongside, and in the same manner, as the incubated samples.
The incubated study sample, fortification and control sample extracts were all analysed by LC-MS/MS with ESI tandem mass spectrometry. Two Multiple Reaction Monitoring (MRM) transitions were monitored to confirm the selectivity of the method. The first transition was used for quantification and the second for confirmation. For each analytical run a calibration curve using matrix matched standards of known concentration in the range ca 0.25 to 51.0 ng/mL was constructed to allow quantification of the test substance in the sample extracts.
The concentrations of extractable test substance remained essentially unchanged throughout the duration of the study. The nominal concentration was 0.35 mg/kg and the average measured concentration for the initial time point (zero-time) samples was 0.25 mg/kg or 73% of nominal which remained at 0.29 mg/kg or 81% of nominal after 120 days.
The degradation rate and the DT50/DT90 values of the test substance in the four test soils were determined according to the FOCUS guidance document on degradation kinetics using the KinGUI software. The test substance was shown to have no significant degradation with estimated DT50 values of greater than 1000 days in all soils.
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