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EC number: 203-137-6 | CAS number: 103-71-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- Principles of method if other than guideline:
- The micronucleus test was employed to investigate phenyl isocyanate in male and female mice for a possible clastogenic effect on the chromosomes of bone-marrow erythroblasts. The known clastogen and cytostatic agent, cyclophosphamide, served as positive control. The treated animals received a single intraperitoneal administration of either phenyl isocyanate or cyclophosphamide.
The femoral marrow of groups treated with phenyl isocyanate was prepared 24, 48 and 72 hours after administration. All negative and positive control animals were sacrificed after 24 hours. The doses of phenyl isocyanate and the positive control, cyclophosphamide, were 30 and 20 mg/kg body weight, respectively. - GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- Phenyl isocyanate
- EC Number:
- 203-137-6
- EC Name:
- Phenyl isocyanate
- Cas Number:
- 103-71-9
- Molecular formula:
- C7H5NO
- IUPAC Name:
- isocyanatobenzene
- Details on test material:
- content: 99.9%
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- NMRI
- Sex:
- male/female
Administration / exposure
- Route of administration:
- intraperitoneal
- Duration of treatment / exposure:
- The femoral marrow of groups treated with phenyl isocyanate was prepared 24, 48 and 72 hours after administration.
- Frequency of treatment:
- single intraperitoneal administration
- Post exposure period:
- Animals were sacrificed 24, 48 and 72 hours after the administration, and the femoral marrow was prepared
Doses / concentrationsopen allclose all
- Dose / conc.:
- 0 mg/kg bw/day
- Dose / conc.:
- 30 mg/kg bw/day
- No. of animals per sex per dose:
- 5 male and 5 female mice/dose
- Control animals:
- yes, concurrent vehicle
Examinations
- Tissues and cell types examined:
- Femoral marrow
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- yes
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
The animals treated with phenyl isocyanate showed lasting symptoms of toxicity after administration. One of forty animals died before the end of the test due to the acute intraperitoneal toxicity of 30 mg/kg phenyl isocyanate. There was an altered ratio between polychromatic and normochromatic erythrocytes.
No indications of a relevant clastogenic effect of phenyl isocyanate were found after a single intraperitoneal treatment with 30 mg/kg.
Cyclophosphamide, the positive control, had a clear clastogenic effect, as is shown by the biologically relevant increase in polychromatic erythrocytes with micronuclei. The ratio of polychromatic to normochromatic erythrocytes was not altered.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results: negative
- Executive summary:
The micronucleus test was employed to investigate phenyl isocyanate in male and female mice for a possible clastogenic effect on the chromosomes of bone-marrow erythroblasts. The known clastogen and cytostatic agent, cyclophosphamide, served as positive control.
The treated animals received a single intraperitoneal administration of either phenyl isocyanate or cyclophosphamide. The femoral marrow of groups treated with phenyl isocyanate was prepared 24, 48 and 72 hours after administration. All negative and positive control animals were sacrificed after 24 hours. The doses of phenyl isocyanate and the positive control, cyclophosphamide, were 30 and 20 mg/kg body weight, respectively.
The animals treated with phenyl isocyanate showed lasting symptoms of toxicity after administration. One of forty animals died before the end of the test due to the acute intraperitoneal toxicity of 30 mg/kg phenyl isocyanate.
There was an altered ratio between polychromatic and normochromatic erythrocytes. No indications of a relevant clastogenic effect of phenyl isocyanate were found after a single intraperitoneal treatment with 30 mg/kg.
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