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EC number: 221-576-1 | CAS number: 3148-73-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
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- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
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- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
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- Nanomaterial porosity
- Nanomaterial pour density
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- Nanomaterial catalytic activity
- Endpoint summary
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- Environmental data
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
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- Additional toxicological data
Toxicity to other aquatic organisms
Administrative data
Link to relevant study record(s)
Description of key information
Acute amphibian toxicity: LC50=18.96g/L, 96h, embryos of the African clawed frog, FETAX (ASTM E1439-98), Schultz 1998
Sublethal effects: EC50=12.72g/L, 96h, tadpoles of the African clawed frog, FETAX (ASTM E1439-98), Schultz 1998
Additional information
Schultz et al (1988) investigated the test item, for lethal and morphological effects (teratogenicity, malformations) to embryos of the African clawed frog (Xenopus laevis). The non-GLP study was conducted according to the Frog Embryo Teratogenesis Assay-Xenopus (FETAX) protocol according to ASTM E1439-98. The experiments are considered “reliable with restrictions” (Klimisch 2), conclusive with regard to the establishment of acute lethal and sublethal effects and thus adequate for risk assessment and classification and labelling.
The test organisms in the stage of early to mid-blastula embryos were exposed via the aqueous medium during 96 hours to a series of not further specified graduated concentrations of the test chemicals or pure medium (control). The effects were compared to the reference substance (positive control) Semicarbazide hydrochloride (CAS 563-41-7), from which teratogenic effects were well known as evidenced by the authors, and which represents the common structural element of the substances investigated by Schultz et al (1988). The specific effect of the reference is Osteolathyrism, a collagen cross-linking deficiency. Accordingly histological and electron microscopical examinations were conducted on representative embryos with emphasis on the axial skeleton. Additionally developmental stage, length, macroscopic malformations and mortality were recorded.
The test item was able to induce osteolathyrism (Mekenyan et al 1996) and gave thus a positive teratogenic response with a 96 h EC50 of 12.72 g/L, which is 109.51 mmol/L. This EC50 indicates a 2190 times less effectiveness than the positive control on molar basis. The 96 h LC50 of the test item was determined to be 18.96 g/L or 163.30 mmol/L, which is less toxic than the reference substance by a factor of 2.86 on molar basis.
In conclusion the test item did not exhibit acute toxicity to aquatic amphibians up to the cut-off limit of 100 mg/L and no classification or labelling requirement is implicated by the results of this study. As the 50 % teratogenic response was observed at concentrations in the order of magnitude of lethality and a 2190 times higher molarity than the reference, osteolathyrism is not considered a relevant effect of the test item at environmental concentrations.
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