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EC number: 210-868-4 | CAS number: 624-89-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Ethyl methyl sulphide
- EC Number:
- 210-868-4
- EC Name:
- Ethyl methyl sulphide
- Cas Number:
- 624-89-5
- Molecular formula:
- C3H8S
- IUPAC Name:
- (methylsulfanyl)ethane
- Details on test material:
- Source: Elf Atochem, RDAM
Batch: MES 2611-11933
Purity: 98.4%
Constituent 1
Method
- Target gene:
- Histidine reversion
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: Strains: TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Metabolic activation system:
- Liver microsomal fraction (S9 fraction) of rats induced with Aroclor 1254
- Test concentrations with justification for top dose:
- Experiment without S9:
. 312.5, 625, 1250, 2500 and 5000 µg/plate: for all tester strains in both experiments except for the TA 1537 and TA 98 strains in the second experiment,
. 156.25, 312.5, 625, 1250 and 2500 µg/plate: for the TA 98 and TA 1537 strains in the second experiment.
Experiments with S9 mix:
. 312.5, 625, 1250, 2500 and 5000 µg/plate: for all tester strain. - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: Without S9 mix: .1 µg/plate of sodium azide (NaN3): TA 1535 and TA 100 strains. 50 µg/plate of 9-Aminoacridine (9AA): TA 1537 strain. 0.5 µg/plate of 2-Nitrofluorene (2NF): TA 98 strain. 0.5 µg/plate of Mitomycin C (MMC): TA 102 strain. With S9 mix: 2 µ
- Details on test system and experimental conditions:
- A preliminary toxicity test was performed to define the dose-levels of ETHYLMETHYL SULFIDE to be used for the mutagenicity study. The test substance was then tested in two independent experiments, with and without a metabolic activation system, the S9 mix, prepared from a liver microsomal fraction (S9 fraction) of rats induced with Aroclor 1254. Both experiments were performed according to the direct plate incorporation method except for the second test with S9 mix, which was performed according to the preincubation method (60 minutes, 37°C). Five strains of bacteria Salmonella typhimurium: TA 1535, TA 1537, TA 98, TA 100 and TA 102 were used. Each strain was exposed to five dose-levels of the test substance (three plates/dose-level). After 48 to 72 hours of incubation at 37°C, the revertant colonies were scored. The evaluation of the toxicity was performed on the basis of the observation of the decrease in the number of revertant colonies and/or a thinning of the bacterial lawn.
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: Strains: TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- >= 2500 µg/ml
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- Since the test substance was freely soluble and almost non-toxic in the preliminary test, the highest dose-level for the first experiment was 5000 µg/plate, according to the criteria specified in the international guidelines. A slight emulsion was sometimes observed in the Petri plates when scoring the revertants at 5000 µg/plate.
Without S9, a slight to marked toxicity was noted (except for the TA 102 strain), depending on the tester strain and the dose-level. The test substance did no induce any noteworthy increase in the number of revertants, in both experiments, in any of the five strains.
With S9, no or slight to moderate toxicity was noted, depending on the tester strain and the dose-level. The test did not induce any noteworthy increase in the number of revertants, in both experiments, in any of the five strains.
The number of reverants for the vehicle and positive controls was as specified in the acceptance criteria. The study was therefore considered valid.
Applicant's summary and conclusion
- Conclusions:
- ETHYLMETHYL SULFIDE does not show mutagenic activity in the bacterial reverse mutation test with Salmonella typhimurium.
- Executive summary:
In a reverse gene mutation assay in bacteria (# OECD 471, GLP), ethyl methyl sulfide was tested in the bacterial reverse mutation assay (OECD TG 471) with Salmonella typhimurium TA98, TA100, TA102, TA1535, and TA1537 at concentrations up to 5000 µg/plate with and without metabolic activation. The solvent was dimethylsulfoxide (DMSO). Slight cytotoxicity was observed at concentrations >= 2500 µg/plate. Positive controls produced the expected result. Ethyl methyl sulfide was not mutagenic under the conditions of the assay.
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