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EC number: 466-490-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experiment start date - 24 July 2006; Experiment completion date - 22 August 2006; Study completion date - 25 September 2006.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Identity: FAT 40827/A
Batch: T2 5572 BOP 01/06
Purity: determined in this study
Appearance: black sticky powder
Expiration date: 28.02.2011
Storage: at room temperature - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- The sludge was washed three times with tap water by centrifugation and the supernatant liquid phase was decanted. A homogenized aliquot of the final sludge suspension was weighed, thereafter dried and the ratio of wet to dry weight was calculated. The final concentration of inoculum was 30 mg dry material per litre.
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Apparatus: The test flask (500-ml Erlenmeyer flasks) were incubated under continuous stirring in a SAPROMAT D12, oxygen consumption was recorded manually by taking a daily reading at least on each working day.
Test duration: 28 days
Light conditions: darkness
Temperature: 22 °C, maintained with a built-in thermostat and checked once per week.
pH: Prior to test start and at the end of the test, pH value in each flask was determined. - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- sodium benzoate
- Parameter:
- % degradation (O2 consumption)
- Value:
- 3
- Sampling time:
- 28 d
- Remarks on result:
- other: without nitrification
- Parameter:
- % degradation (O2 consumption)
- Value:
- 2
- Sampling time:
- 28 d
- Remarks on result:
- other: with nitrification
- Parameter:
- BOD5
- Value:
- 0 mg O2/g test mat.
- Results with reference substance:
- day 14: 88 %
day 28: 90 % - Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The test substance is not ready biodegradable under valid test conditions.
- Executive summary:
The test item was investigated for its ready biodegradability in a manometric respirometry test over 28 days according to EU Commission Directive 92/69/EEC, C.4 -D (1992) and OECD Guideline for Testing of Chemicals No. 301 F (1992). The test item and reference substance concentrations were 100 mg/l. The test temperature was 22 °C, maintained with a built-in thermostat and checked once per week, and pH was determined prior to test start and at the end of the test in each flask. The result of abiotic control showed that no degradation of test item occurred under the test conditions within 28 days. There was no inhibitory effect on the activity of activated sludge microorganisms from the result of toxicity control. The biochemical oxygen demand (BOD) of the test item in the test media was in the range of the inoculum controls throughout the entire 28-day exposure period. The biodegradation of reference substance was 88 % on day 14 and reached 90 % on day 28. Consequently, test item is not ready biodegradable under the valid test conditions within 28 days.
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experiment start date - 28 August 2006; Experiment completion date - 29 September 2006; Study completion date - 28 November 2006.
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: OECD guidance test in compliance with GLP, but the inoculum was collected only from one source, the test water was different from the requirement of OECD 302C and the test temperature was also not the required temperature according to OECD 302C.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))
- Deviations:
- yes
- Remarks:
- activated sludge from only one source; the test water was prepared according to OECD 301F; the temperature was 22 °C; the inoculum was not fed during the holding period; the holding period was maximum seven days; no test item specific analysis
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Identity: FAT 40827/A
Batch: T2 5572 BOP 01/06
Purity: determined in this study
Appearance: black sticky powder
Expiration date: 28.02.2011
Storage: at room temperature - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- The sludge was washed twice with tap water by centrifugation and the supernatant liquid phase was decanted. A homogenized aliquot of the final sludge suspension was weighed, thereafter dried and the ratio of wet to dry weight was calculated. The final concentration of inoculum was 100 mg dry material per litre.
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 50 mg/L
- Based on:
- test mat.
- Initial conc.:
- 51 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Apparatus: the test flask (500-ml Erlenmeyer flasks) were incubated under continuous stirring in a SAPROMAT D12, oxygen consumption was recorded manually by taking a daily reading at least on each working day
Test duration: 28 days light conditions: darkness temperature: 22 °C, maintained with a built-in thermostat and checked once per week
pH: prior to test start and at the end of the test, pH value in each flask was determined. - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- sodium benzoate
- Parameter:
- % degradation (O2 consumption)
- Value:
- 13
- Sampling time:
- 28 d
- Remarks on result:
- other: without nitrification
- Parameter:
- % degradation (O2 consumption)
- Value:
- 8
- Sampling time:
- 28 d
- Remarks on result:
- other: with nitrification
- Results with reference substance:
- Day 14: 78 %
Day 28: 86 % - Validity criteria fulfilled:
- yes
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- At the end of the 28-day test period, the mean biodegradation of test item amounted to 13 % without nitrification and 8 % with nitrification. In conclusion, test item is not inherently biodegradable under the valid test conditions within 28 days.
- Executive summary:
The test item was investigated for its inherent biodegradability in a manometric respirometry test over 28 days based on the OECD Guideline for Testing of Chemicals No. 302 C (1981). The test temperature was 22 °C, maintained with a built-in thermostat and checked once per week, and pH was determined prior to test start and at the end of the test in each flask. The result of abiotic control showed that no degradation of test item occurred under the test conditions within 28 days. There was no inhibitory effect on the activity of activated sludge microorganisms from the result of toxicity control. The biochemical oxygen demand (BOD) of the test item in the test media was in the range of the inoculum controls in one of the two replicates and slightly above the range of the inoculum controls in the other replicate throughout the entire 28-day exposure period. At the end of the 28-day test period, the mean biodegradation of test item amounted to 13 % without nitrification and 8 % with nitrification. The biodegradation of reference substance was 78 % on day 14 and reached 86 % on day 28. Consequently, test item is not biodegradable under the valid test conditions within 28 days.
Referenceopen allclose all
In the toxicity control, biodegradation amounted to 53 % and 41 % without nitrification and with nitrification, respectively. Thus, the test item had no inhibitory effect on activated sludge microorganisms at the tested concentration of 100 mg/l, because biodegradation in the toxicity control was >25 % within 14 days.
In the toxicity control, biodegradation amounted to 62 % and 53 % without nitrification and with nitrification, respectively. Thus, the test item had no inhibitory effect on activated sludge microorganisms at the tested concentration of 50 mg/l, because biodegradation in the toxicity control was >25 % within 14 days.
Description of key information
The test substance is not readily or inherently biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Two biodegradability tests are available for this substance, one investigating ready biodegradability according to OECD 301F and one inherent biodegradability according to OECD302C. The results are summarized as below. The ready biodegradability test was performed over 28 days according to EU Commission Directive 92/69/EEC, C.4 -D (1992) and OECD Guideline for Testing of Chemicals No. 301 F (1992). There was no inhibitory effect on the activity of activated sludge microorganisms from the result of toxicity control. The biochemical oxygen demand (BOD) of the test item in the test media was in the range of the inoculum controls throughout the entire 28-day exposure period. Consequently, test item is not ready biodegradable under the valid test conditions within 28 days.
Additionally, the test item was investigated for its inherent biodegradability in a manometric respirometry test over 28 days based on the OECD Guideline for Testing of Chemicals No. 302 C (1981). The test temperature was 22 °C, maintained with a built-in thermostat and checked once per week, and pH was determined prior to test start and at the end of the test in each flask. The result of abiotic control showed that no significant degradation of test item occurred under the test conditions within 28 days. There was no inhibitory effect on the activity of activated sludge microorganisms from the result of toxicity control. The biochemical oxygen demand (BOD) of the test item in the test media was in the range of the inoculum controls in one of the two replicates and slightly above the range of the inoculum controls in the other replicate throughout the entire 28-day exposure period. At the end of the 28-day test period, the mean biodegradation of test item amounted to 13 % without nitrification and 8 % with nitrification. The biodegradation of reference substance was 78 % on day 14 and reached 86 % on day 28. Consequently, test item is not inherently biodegradable under the valid test conditions within 28 days.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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