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EC number: 442-680-5 | CAS number: 443688-20-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July 22nd to 24th, 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Red LF 6339
- IUPAC Name:
- Red LF 6339
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected: ARA Ergolz II, Fullinsdorf, Switzerland
- Pretreatment: the sludge was washed twice with tap water by centrifugation and the supernatant liquid phase was decanted. A homogenised aliquot of the final sludge suspension was weighed thereafter dried and the ratio of wet to dry weight was calculated. Based on ths ratio, an aliquot of washed sludge was suspended in tap water to obtain a concentration equivalent to 3 g dry material per liter. During the holding of two days prior to use, the sludge was fed daily with 50 ml synthetic wastewater per liter and was kept under continuous aeration until use at RT. Immediately before use, the dry weight of the activated sludge was measured again in the inoculum used in the test. The pH of the activated sludge inoculum was adjusted from 7.9 to 7.1 with a diluted sulfuric acid solution.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 22 °C (start)
19 °C (end) - Dissolved oxygen:
- did not drop below 2.5 mg/l during the incubation period
just before the measurement of the respiration rates the dissolved oxugen was at least 8.3 mg/l - Nominal and measured concentrations:
- 10, 32, 100, 320 and 1000 mg/l (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1000 ml glass flasks
- Material, size, headspace, fill volume: 200 ml activate sludge inoculum was added. the sludge was added in the intervals of 15 minutes first to a control, then to test solution of the reference item then to the test solutions of the test item and finally to a second control.
- Aeration: continuously aerated with compressed air at a flow appr. one liter per minute.
- No. of vessels per concentration (replicates): one
- No. of vessels per control (replicates): 2
- Sludge concentration (weight of dry solids per volume): 2.8 g/l dry weight corresponding to 1.1 g dry material per litre test medium
- Nitrification inhibitor used (delete if not applicable): none / N-allylthiourea
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:synthetic water as per the test guidelines
- Intervals of water quality measuremets: the pH values and the dissolved oxygen were determined at the start and at the end of the incubation The water temperature was measured at the start and at the end of the incubation in one control, and was continuously monitored in all test media and the controls during measurement of the respiration rates. Before the addition of the activated sludge, the appearance of the test media was recorded.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : a well-mixed sample of each test medium was poured into a BOD-flask after exactly 3 hours incubation time, and was not further aerated. The dissolved oxygen concentration was measured with an oxygen electrode and was continuouslly recorded for about ten to fifteen minutes. During measurement, the samples were stirred on a magnetic stirrer. The oxugen consumption rate was determined from the linear part of the respiration curve in the range of 6.2-2.5 mg/l oxygen/l.
TEST CONCENTRATIONS
- Spacing factor for test concentrations:3.2
- Reference substance: 5, 16 and 50 mg/l - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Details on results:
- The test item had no significant inhibitory effect (< 15 %) on the respiration rate.
The oxygen consumption rates of the two controls differend only by 0.2 % - Results with reference substance (positive control):
- EC50 (3h) = 11.5 mg/l (95 % CL: 10.7-12.4 mg/l)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- EC50 (3h) > 1000 mg/l (nominal)
EC20 (3h) > 1000 mg/l (nominal)
NOEC (3h) = 1000 mg/l (nominal) - Executive summary:
The inhibitory effect of the test item on the respiration rate of aerobic wastewater microorganisms of activated sludge was investigated in a 3-hour respiration inhibition test according to the EU Commission Directive 87/302/EEC, and the OECD Guideline for Testing of Chemicals, No. 209. The following nominal concentrations were tested: 10, 32, 100, 320 and 1000 mg/l. Concentrations exceeding 1000 mg/l were not tested. In addition, two controls and three different concentrations of the reference item 3,5-dichlorophenol (5, 16 and 50 mg/l) were tested in parallel. The results of these treatments confirmed suitability of the activated sludge and the method used.
Up to and including the highest concentration of 1000 mg/l the test item had no significant inhibitory effect ( <15%) on the respiration rate of activated sludge after the incubation period of three hours. Thus, the 3-hour NOEC of the test item to activated sludge microorganisms was at least 1000 mg/l. This value might be even higher, but concentrations exceeding 1000 mg/l were not tested. The 3-hour EC20, EC50 and EC80 could not be calculated, but were clearly higher than 1000 mg/l.
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