Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 695-953-8 | CAS number: 204918-22-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- prop-2-en-1-yl 2-(2-chloro-5-isocyanatobenzoyloxy)-2-methylpropanoate
- EC Number:
- 695-953-8
- Cas Number:
- 204918-22-9
- Molecular formula:
- C15H14ClNO5
- IUPAC Name:
- prop-2-en-1-yl 2-(2-chloro-5-isocyanatobenzoyloxy)-2-methylpropanoate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: Clone 5
- Source: Test facility laboratory
- Age at study initiation: <24 hours
Study design
- Test type:
- static
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 214 mg/L CaCO3
- Test temperature:
- 20°C ± 2°C, constant throughout the test within ± 1°C
- pH:
- 8.0 - 8.1
Alkalinity: 0.8 mmol/l - Dissolved oxygen:
- Saturation at the begining of the test. 96 - 100 % during the test.
- Nominal and measured concentrations:
- Nominal concentrations: 0.4, 0.9, 1.9, 4.3 and 9.4 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 ml beaers containing 100 ml test medium.
- No. of organisms per concentration: 20 Daphnia per concentration, two beakers per test concentration with 10 animlas each.
TEST MEDIUM / WATER PARAMETERS
The test medium was prepared with bi-distilled water and had the composition as detailed in Table 1.
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light / 8 hours dark.
EFFECT PARAMETERS MEASURED : For measurement refer to Table 3.
Oxygen: Oxygen content was measured at the start and at the end of the exposure period.
Temperature: Temperature was measured at the start and at the end of the exposure period.
pH: The pH was measured at the start and at the end of exposure period.
Immobilisation: The immobilisation of the Daphnia was recorded after 24 and 48 hours of exposure.
Those animals not able to swim within 15 seconds after gentle agitation of the test vessel were
considered immobile.
TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline:
It was not possible to make a stock solution more concentrated than 10 % in DMF because of the
limited solubility of the test item CA 2814 B in DMF. Since the guidelines set the limit for the solvent
DMF at 0.01% in water, the test item concentration of 9.4 mg/L was the highest possible
concentration in the test.
- Range finding study: A range finding test was performed with concentrations of 0.1, 1.0 and 10 mg/L.
- Test concentrations: The main test was performed with nominal test item concentrations of 0.4, 0.9, 1.9, 4.3 and 9.4 mg/L in addition of blank control and a DMF control (0.01%), both without test item. Calculated amounts
of the stock solution were given into the test vessels to produce the desired test concentrations and
then made up to 200m with test medium. The200ml of test solutions were divided into two portions
of 10ml for each replicate test vessel. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 9.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- ca. 4.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 9.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- ca. 9.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- No immobilisation of the Daphnia was observed in the control and in the test item concentrations of 0.4, 0.9, 1.9 and 4.3 mg/L after the test period of 48 hours. Only at the highest test item concentration of 9.4 mg/L, 25 % of the Daphnia were immobilised after 48 hours. After 48 hours exposure no sublethal effects on the daphnia were recorded.
Number of immobilisaed daphnia after 24 and 48 hours refer to Table 2. - Results with reference substance (positive control):
- Reference control on April 09, 2001: EC50 1.4 mg/L.
- Reported statistics and error estimates:
- Due to the absence of 50% mortality at the highest test item concentrations, the EC50 values were estimated without statistical program.
Any other information on results incl. tables
Table 2:
Mean measured concentration of test item (mg/L) |
No of daphnia |
No of immobilised daphnia |
Immobility (%) |
||
24 h |
48 h |
24 h |
48 h |
||
Blank + DMF |
10 10 |
0 0 |
0 0 |
0 |
0 |
Blank |
10 10 |
0 0 |
0 0 |
0 |
0 |
0.4 |
V |
0 0 |
0 0 |
0 |
0 |
0.9 |
10 10 |
0 0 |
0 0 |
0 |
0 |
1.9 |
10 10 |
0 0 |
0 0 |
0 |
0 |
4.3 |
10 10 |
0 0 |
0 0 |
0 |
0 |
9.4 |
10 10 |
0 0 |
3 2 |
0 |
25 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- not mote than 10% of the daphnia in the controls were immobilised
- Conclusions:
The EC-values are based on the nominal concentrations of CA 2814 B:
Exposure period EC values (mg/L)
EC 0 EC 50 95% confidence limits of EC50
24 hours 9.4 >9.4 n.d
48 hours 4.3 >9.4 n.d
n.d.: could not be determined- Executive summary:
The water temperature was maintained at 20.2 – 21.0 °C throughout the exposure period in all test vessels. The oxygen content was in the range of 96 – 100% during the test and the pH ranged from 8.0 – 8.1.
Since the test item is instable in water it was not possible to make a stock solution of CA 2814 Bin water (Pfeifle, 2001). Hence, N,N-Dimethylformamide (DMF) was used as solubilising agent for the preparation of a 10% stock solution (0.5 g CA 2814 B in 5 ml DMF). The following nominal concentrations were chosen for the definitive test 0.4, 0.9, 1.9, 4.3 and 9.4 mg/Lin addition to a blank control and a DMF-control, both without test item. It was not possible to make a stock solution more concentrated than 10 % in DMF because of the limited solubility of the test item CA 2814 B in DMF. Since the guidelines set the limit for the solvent DMF at 0.01% in water, the test item concentration of 9.4 mg/L was the highest possible concentration in the test.
Due to the instability of the test item (Pfeifle, 2001), the actual test concentrations of CA 2814 B were not determined analytically. Hence, the results were calculated with the nominal concentrations.
No immobilisation of the Daphnia was observed in the control and in the test item concentrations of 0.4, 0.9, 1.9 and 4.3 mg/L after the test period of 48 hours. Only at the highest test item concentration of 9.4 mg/L, 25 % of the Daphnia were immobilised after 48 hours. After 48 hours exposure no sublethal effects on the daphnia were recorded.
The EC-values are based on the nominal concentrations of CA 2814 B:
Exposure period
EC values (mg/L)
EC 0
EC 50
95% confidence limits of EC50
24 hours
9.4
>9.4
n.d
48 hours
4.3
>9.4
n.d
n.d.: could not be determined
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.