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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1982
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: well documented guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1982
Report date:
1982

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Version / remarks:
adopted may 12th, 1981
Deviations:
no
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Ethyl diphenylphosphinite
EC Number:
211-951-8
EC Name:
Ethyl diphenylphosphinite
Cas Number:
719-80-2
Molecular formula:
C14H15OP
IUPAC Name:
ethyl diphenylphosphinite
Details on test material:
- Name of test material (as cited in study report): Diphenylethoxyphopsphin
- Physical state: liquid
- Expiration date of the lot/batch: 10/1982

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Dr. K. Thomae GmbH, Biberach
- Age at study initiation: app. 12 weeks
- Weight at study initiation: 180g on average
- Fasting period before study: 16h (water ad lib.)
- Housing: in groups of 5 in V-II-A-meshed steel cages, type DK-III
- Diet (e.g. ad libitum): SSNIFF
- Water (e.g. ad libitum): VE water or tap water (only on holidays) ad lib.
- Acclimation period: at least 1 week

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-26°C
- Humidity (%): 45 - 75%
- Air changes (per hr): 15-20
- Photoperiod (hrs dark / hrs light): 12h/12h

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
other: 0.5% CMC in water
Details on oral exposure:
VEHICLE
- Concentration in vehicle: 6.81; 4.64; 3.16; 2.15% (w/v)
- Amount of vehicle (if gavage): 10ml/kg b.w.
- Justification for choice of vehicle: aqueous solution comparable to physiological media

MAXIMUM DOSE VOLUME APPLIED: 10ml/kg b.w.
Doses:
681mg/kg
464mg/kg
316mg/kg
215mg/kg
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations: >15min, 15min, 30min, 1h, 2h, 4h, and 5h after application, twice daily on workdays and daily on holidays thereafter
- Frequency of weighing: day 0, 2, 7, 13
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, histopathology of findings during gross pathology
Statistics:
LD50 (m+f) calculated according to Finney, D.J., probit analysis, cambridge university press, 1971

Results and discussion

Effect levelsopen allclose all
Sex:
female
Dose descriptor:
LD50
Effect level:
ca. 316 mg/kg bw
Based on:
test mat.
Sex:
male
Dose descriptor:
LD50
Effect level:
ca. 681 mg/kg bw
Based on:
test mat.
Sex:
male/female
Dose descriptor:
LD50
Effect level:
ca. 456 mg/kg bw
Based on:
test mat.
95% CL:
> 357 - < 650
Mortality:
Males
681mg/kg: 3 animals died within 24h, no mortality occured in any other test group
Females
681mg/kg, 464mg/kg: all except one animal of the highest dose died within 24h, the last animal died within 2days
316mg/kg: 2 animals died within 24h
215mg/kg: 1 animal died within 24h
Clinical signs:
Dyspnoe, apathy, staggering gait, impaired general state in all groups
abnormal position, atony, tonic cramps, piloerection, exsiccosis , exophthalmus in some groups
no corneal reflex, no pain reflex, anaesthesia-like state in females in the two mid dosages
for details see tables 1 and 2 under additional information
Body weight:
normal weight gain in all male survivers
stagnation in females up to day 2, normal weight gain thereafter
Gross pathology:
Discoloration (whitish grey to clay colored) of liver and kidney in animals found dead.
Marks on the peripheral lobuli of the liver in 2 surviving male animals receving 464mg/kg, diagnosed as lipophanerosis during histopathological examination.
No further abnormalities were noted.

Any other information on results incl. tables

Table 1

Males
  681mg/kg 464mg/kg 316mg/kg 215mg/kg
Dyspnoe 15m-2d 15m-2d 15m-2d 15m-2d
Apathy 15m-2d 15m-2d 15m-2d 15m-2d
abnormal position 1h-5h 2h-1d    
staggering gait 30m-2d 30m-2d 30m-2d 30m-5h
Atony 1h-5h 2h-1d    
tonic cramps 2h-5h 2h-5h    
piloerection 30m-6d   2h-6d 2d-7d
exsiccosis 1h-5h 2h-2d 1d-2d  
exophthalamus 2h-5h 2h    
impaired general state 15m-2d 15m-2d 15m-2d 15m-2d
Table 2
Females
  681mg/kg 464mg/kg 316mg/kg 215mg/kg
Dyspnoe 15m-1d 15m-5h 15m-2d 15m-2d
Apathy 15m-1d 15m-5h 15m-2d 15m-2d
abnormal position 1h-5h 1h-5h 2h-1d  
staggering gait 30m-1d 30m-5h 30m-2d 30m-2d
Atony 15m-1d 1h-5h 2h-1d  
no pain reflex   2h 2h  
no corneal reflex   2h 2h  
anaesthesia-like state   2h 2h  
tonic cramps 2h-5h   2h-5h  
piloerection 30m-5h 2h-5h 2h-6d 2h-6d
exsiccosis 2h-5h 1h-5h 2h-2d 2d
exophthalamus 2h-5h 2h-5h 2h-1d  
impaired general state 15m-1d 15m-5h 15m-2d 15m-2d

m: minute, h: hour, d: day

Applicant's summary and conclusion

Interpretation of results:
harmful
Remarks:
Migrated information