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Diss Factsheets
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EC number: 935-211-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study with analytical concentration control
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1,4:3,6-dianhydro-2,5-bis-O-(diphenoxyphosphoryl)-D-glucitol
- EC Number:
- 935-211-5
- Cas Number:
- 1305113-15-8
- Molecular formula:
- C30H28O10P2
- IUPAC Name:
- 1,4:3,6-dianhydro-2,5-bis-O-(diphenoxyphosphoryl)-D-glucitol
- Details on test material:
- - Name of test material (as cited in study report): Isosorbid-O,O'-bis(diphenylphosphorsäureester)
- Physical state: solid
- Analytical purity: 97.9 Mol-%
- Storage condition of test material: ambient
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: Sampling for concentration control was done at the start of the exposure (0 h) from replicate A and at the end of the exposure from replicate B.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
The test substance is poorly soluble in water. Therefore the test solution was prepared using a saturation column in order to achieve a saturated solution of the test substance. The saturation column was prepared by weighing approx. 200 mg of test substance into a weighing boat and dissolving this mass in approx. 200 mL dichloromethane. The solvent solution was then poured over the pre-cleaned quartz wool (approx. 20 g) in a stainless steel pan and the solvent was completely evaporated leaving the test substance adhered to the glass wool. The loaded quartz wool is rolled and packed into a glass column (50 cm long with 2.8 cm internal diameter) fitted with a plug of clean quartz wool to keep particulate matter in the column. Silicon stoppers fitted with short glass tubes were attached to the ends of the column, which was then connected to a peristaltic pump (Watson Marlow GMBH, Germany, 500 series) via a short length flexible tubing (Watson Marlow GmbH, Germany, Marprene). The packed column was rinsed with dilution water (column flow rate 333 mL/h) for about one day and the rinse water was discarded. The test was carried out in a flow-through system. The outflow of the column was introduced into a stock solution tank with an overflow , from which it was distributed equally among the 100% test vessels using a multichannel dispensing pump (Ismatec SA Switzerland, IPC series).
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the test laboratory
- Age at study initiation: < 24 h (at least 3rd brood progeny)
- Feeding during test: none
HOUSING
- Conditions (same as test or not): same as test
- Type and amount of food: Unicellular green algae, Desmodesmus subspicatus
- Feeding frequency: daily
- Renewal of the culture water: twice weekly
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 2.52 - 2.38 mmol/L
- Test temperature:
- 19.6 - 20.1 °C
- pH:
- 7.6 - 7.7
- Dissolved oxygen:
- 9.0 - 9.3 mg/L
- Nominal and measured concentrations:
- Nominal: 0.3 mg/L (and control)
Measured at 0 h: 0.207 mg/L
Measured at 48 h: 0.217 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: flow-through chamber
- Aeration: none
- Type of flow-through: For each replicate, test solutions are pumped via peristaltic pump into a mixing chamber. The solution flows continuously from the mixing chamber into the flow-through chamber (test vessel)
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic freshwater
- Conductivity: 589 - 606 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH and dissolved oxygen at the start and at the end; temperature continuously
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light : 8 hours darkness
- Light intensity: ca. 20 - 850 µS/cm
EFFECT PARAMETERS MEASURED
Each test vessel was visually checked for immobilized daphnids or any abnormal behaviour after 0, 24 and 48 hours.
TEST CONCENTRATIONS
- Results used to determine the conditions for the definitive study: In a preliminary test at a nominal concentration of 100 mg/L, 90% survival was obtained after 48 hours. Since the substance is known to be poorly soluble in water, only a saturated solution was tested. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (K2Cr2O7)
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.212 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: saturated solution
- Details on results:
- - A saturated solution was tested. No effects were observed within the range of solubility.
- Results with reference substance (positive control):
- EC50 = 1.15 mg/L (determined 2011-08-29)
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.