1,4:3,6-dianhydro-2,5-bis-O-(diphenoxyphosphoryl)-D-glucitol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study with analytical concentration control

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,4:3,6-dianhydro-2,5-bis-O-(diphenoxyphosphoryl)-D-glucitol

EC Number:

935-211-5

Cas Number:

1305113-15-8

Molecular formula:

C30H28O10P2

IUPAC Name:

1,4:3,6-dianhydro-2,5-bis-O-(diphenoxyphosphoryl)-D-glucitol

Details on test material:

- Name of test material (as cited in study report): Isosorbid-O,O'-bis(diphenylphosphorsäureester)
- Physical state: solid
- Analytical purity: 97.9 Mol-%
- Storage condition of test material: ambient

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Sampling method: Sampling for concentration control was done at the start of the exposure (0 h) from replicate A and at the end of the exposure from replicate B.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
The test substance is poorly soluble in water. Therefore the test solution was prepared using a saturation column in order to achieve a saturated solution of the test substance. The saturation column was prepared by weighing approx. 200 mg of test substance into a weighing boat and dissolving this mass in approx. 200 mL dichloromethane. The solvent solution was then poured over the pre-cleaned quartz wool (approx. 20 g) in a stainless steel pan and the solvent was completely evaporated leaving the test substance adhered to the glass wool. The loaded quartz wool is rolled and packed into a glass column (50 cm long with 2.8 cm internal diameter) fitted with a plug of clean quartz wool to keep particulate matter in the column. Silicon stoppers fitted with short glass tubes were attached to the ends of the column, which was then connected to a peristaltic pump (Watson Marlow GMBH, Germany, 500 series) via a short length flexible tubing (Watson Marlow GmbH, Germany, Marprene). The packed column was rinsed with dilution water (column flow rate 333 mL/h) for about one day and the rinse water was discarded. The test was carried out in a flow-through system. The outflow of the column was introduced into a stock solution tank with an overflow , from which it was distributed equally among the 100% test vessels using a multichannel dispensing pump (Ismatec SA Switzerland, IPC series).

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the test laboratory
- Age at study initiation: < 24 h (at least 3rd brood progeny)
- Feeding during test: none

HOUSING
- Conditions (same as test or not): same as test
- Type and amount of food: Unicellular green algae, Desmodesmus subspicatus
- Feeding frequency: daily
- Renewal of the culture water: twice weekly

Study design

Test type:

flow-through

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

2.52 - 2.38 mmol/L

Test temperature:

19.6 - 20.1 °C

pH:

7.6 - 7.7

Dissolved oxygen:

9.0 - 9.3 mg/L

Nominal and measured concentrations:

Nominal: 0.3 mg/L (and control)
Measured at 0 h: 0.207 mg/L
Measured at 48 h: 0.217 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: flow-through chamber
- Aeration: none
- Type of flow-through: For each replicate, test solutions are pumped via peristaltic pump into a mixing chamber. The solution flows continuously from the mixing chamber into the flow-through chamber (test vessel)
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic freshwater
- Conductivity: 589 - 606 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH and dissolved oxygen at the start and at the end; temperature continuously

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light : 8 hours darkness
- Light intensity: ca. 20 - 850 µS/cm

EFFECT PARAMETERS MEASURED
Each test vessel was visually checked for immobilized daphnids or any abnormal behaviour after 0, 24 and 48 hours.

TEST CONCENTRATIONS
- Results used to determine the conditions for the definitive study: In a preliminary test at a nominal concentration of 100 mg/L, 90% survival was obtained after 48 hours. Since the substance is known to be poorly soluble in water, only a saturated solution was tested.

Reference substance (positive control):

yes

Remarks:

potassium dichromate (K2Cr2O7)

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.212 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: saturated solution

Details on results:

- A saturated solution was tested. No effects were observed within the range of solubility.

Results with reference substance (positive control):

EC50 = 1.15 mg/L (determined 2011-08-29)