Registration Dossier
Registration Dossier
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EC number: 203-309-0 | CAS number: 105-56-6
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP lab testing
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Ethyl cyanoacetate
- EC Number:
- 203-309-0
- EC Name:
- Ethyl cyanoacetate
- Cas Number:
- 105-56-6
- Molecular formula:
- C5H7NO2
- IUPAC Name:
- ethyl 2-cyanoacetate
- Details on test material:
- 99.7% FL. Tank 434
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, adapted
- Details on inoculum:
- Type: Belebtschlanum a predominatly municipal sewage treatment plant(Marl-Oat)
Treatment of the inoculum: 10.07.92
-Sampling from the activated sludge basin of a predominantly domestic sewage cleaning sewage treament plant
-Centrifuge 10 min at 1100g
-Discard the supernatant and resuspend the mud with mineral medium (washing)
-Further centrifugation: 10min at 1100g
-Re-suspension of the slurry. The slurry concentration was 5.0g dry weight per liter. The dry weight was determined before inoculation. During
this time, the sludge aerobic kept tight closure(ventilation through a frit)
- The final concentration of inoculum per test flask was 25mg/l - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 10.84 mg/L
- Based on:
- DOC
- Details on study design:
- Close containers: 2000 ml flask with loose closure, aluminum foil
Experimental approaches 2 vessels with test substance (10.84 mg DOC/l) and inoculum
2 vessels without test substance ,buth with inoculum.
2 vessels with a control substance (sodium benzoate, 9.44mg DOC/l) and inoculum.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (DOC removal)
- Value:
- 98
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- The reference substance benzoic acid, sodium reached after 10days of 10-degradation threshhold a degradation 101%. Suggest sufficient biological activity.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
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