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Diss Factsheets
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EC number: 221-221-0 | CAS number: 3033-77-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Additional information
In vitro studies
EPTAC causes mutations in E. coli WP2 and S. typhimurium 1535, 1537 and 100 but not in 1538 or 98. These mutations did not require metabolic activation to occur. The evidence from the bacterial mutagenicity tests suggests that EPTAC acts as a direct point mutagen by base pair substitution but not frame shift mutation. In addition, tests in two yeast strains have demonstrated that EPTAC can cause gene conversion in two different gene loci.
The positive response in the liver UDS test gives indications of increased DNA damage in mammalian cells as well.
In addition, a well-correlated dose-related increase of sister chromatid exchanges in the Chinese hamster V79 cells was seen.
Damage to chromosomes has been shown to occur in mammalian test systems in vitro. The results of the in vitro chromosome aberration tests in both rat liver cells and Hamster ovary cells showed that both the frequency of aberrations per cell, with or without gaps, and the percentage of cells with all aberrations increased with the dose.
Positive in vitro results show that in addition to causing point mutations in bacterial systems, EPTAC has clastogenic or aneugenic potential in mammalian cells as well.
In vivo study
In an in vivo chromosome aberration test performed according to OECD guideline No 474 and in compliance with GLP, mice (BOR:NMRI) were exposed by intraperitoneal injection to EPTAC at a concentration of 82.5 mg/kg.Positive controls induced the appropriate response.
There was a clear statistically significant increase of micronucleated PCE in males and females.
Under the test conditions, EPATC is clastogenic in mouse in vivo.
Short description of key information:
Positive results in vitro show that in addition to causing point mutations in bacterial systems, EPTAC has clastogenic or aneugenic potential in
mammalian cells as well.
In an in vivo chromosome aberration test in the mouse, EPATC is clastogenic under these conditions .
Endpoint Conclusion: Adverse effect observed (positive)
Justification for classification or non-classification
Harmonised classification
EPTAC is classified as Muta. 2 (H341: Suspected of causing genetic defects) according to the CLP Regulation (EC) N° 1272/2008 and as Muta. Cat. 3 (R68 Possible risk of irreversible effects) according to the Directive 67/548/EEC.
No additional self-classification is proposed.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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