tert-butyl {(1S)-2-[(1S,3S,5S)-3-cyano-2-azabicyclo[3.1.0]hex-2-yl]-1-[3-hydroxyadamantan-1-yl]-2-oxoethyl}carbamate

Density

Administrative data

Endpoint:

density

Remarks:

density

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Between 07 February and 15 March 2012.

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of the relevant results. The study report was conclusive, done to a valid guideline and the study was conducted under GLP conditions.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Materials and methods

Test guideline

Test guideline 1

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method A.3 (Relative Density)

Version / remarks:

using a gas comparison pycnometer, designed to be compatible with Method A3 Relative Density of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 109 of the OECD Guidelines for Testing of Chemicals, 27 July 1995

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 109 (Density of Liquids and Solids)

Version / remarks:

using a gas comparison pycnometer, designed to be compatible with Method A3 Relative Density of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 109 of the OECD Guidelines for Testing of Chemicals, 27 July 1995

Deviations:

no

GLP compliance:

yes

Type of method:

pycnometer method

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

Identification: BMS-528235-01
Description: Off white powder
Batch: 1L63487N
Purity: 98.6 %
Expiry Date: 27 November 2014
Storage Conditions: Room temperature in the dark

Results and discussion

Density

Type:

density

Density:

1 230 kg/m³

Temp.:

21 °C

Any other information on results incl. tables

Results

Calibration

 

The pressure readings and the calculated volume for the reference and sample cells are shown in the following table:

 

Table 3.8

 

Determination	P1	P2	P3	P4	Reference Cell Volume (cm^3)	Sample Cell Volume (cm^3)
1	17.075	6.464	17.002	8.425	90.710	148.906
2	17.051	6.459	17.108	8.482	90.799	148.899
3	17.016	6.445	17.083	8.469	90.775	148.889

 

Sample

 

The pressure readings, calculated volumes and density values obtained for the test item are shown in the following table:

 

Table 3.9

 

Determination	Test	P1	P2	Volume (cm^3)	Density (kg/m^3)	Mean Density (kg/m^3)
1	A	17.128	7.013	17.991	1.24 x 10^3	1.24 x 10^3
	B	17.119	7.010	18.012	1.23 x 10^3
	C	17.570	7.194	17.991	1.24 x 10^3
2	A	17.554	7.214	18.807	1.23 x 10^3	1.23 x 10^3
	B	17.056	7.009	18.796	1.23 x 10^3
	C	17.066	7.012	18.762	1.23 x 10^3
3	A	17.294	7.200	21.655	1.23 x 10^3	1.23 x 10^3
	B	17.012	7.083	21.668	1.23 x 10^3
	C	17.046	7.096	21.632	1.23 x 10^3

 

Temperature:              21.0 ± 0.5 ºC
Mean density:             1.23 x 10³ kg/m³

Applicant's summary and conclusion

Conclusions:

The density of the test item has been determined to be 1.23 x 10E3 kg/mE3 at 21.0 ± 0.5 °C.
.

Executive summary:

The determination was carried out using a gas comparison pycnometer, designed to be compatible with Method A3 Relative Density of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 109 of the OECD Guidelines for Testing of Chemicals, 27 July 1995.

A reference cell with the defined volume (Vref) was pressurised to a typical pressure in the range 16 to 20 psi and the pressure recorded (p1). Following this, the volume of the sample cell was added to the system, resulting in a reduction in pressure, which was also recorded (p2). The volume calibration of the reference cell and the sample cell was confirmed using a stainless steel calibration ball with known volume (Vcal). The test procedure as outlined above was repeatedwith the sample cell containing the calibrated ball (pressure readings p3 and p4 for the upper and lower pressure values, respectively).

 

From these four pressure readings, the volume of the reference cell (Vref) and of the sample cell (Vcell) was calculated as outlined in data handling. The calibration was performed at least on each day of measurement. The temperature was monitored and recorded.

 

Both determinations were performed in triplicate.

   

Conclusion

The density of the test item has been determined to be 1.23 x 10³ kg/m³at 21.0 ± 0.5 °C.