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EC number: 700-578-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- The reliability is rated 2 because the study followed a standard guideline of reference (NF EN ISO 6341), which describes a procedure designed to evaluate this endpoint. The results were reviewed for reliability and assessed as valid. Howerver the study was not conducted under GLP conditions.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- GLP compliance:
- no
Test material
- Reference substance name:
- Mono and bis and tris{tris[4-(mono and dimethylamino)phenyl]methylium} [12,21,30,32-tetrahydro-29H,31Hphthalocyanine- mono, bis and trisulfonato-k4N29,N30,N31,N32]cuprate.
- IUPAC Name:
- Mono and bis and tris{tris[4-(mono and dimethylamino)phenyl]methylium} [12,21,30,32-tetrahydro-29H,31Hphthalocyanine- mono, bis and trisulfonato-k4N29,N30,N31,N32]cuprate.
- Details on test material:
- - Name of test material (as cited in study report): Sepisol Fast Violet 2B
- Lot/batch No.: 423393
- Substance type: organocopper
- Physical state and appearance: violet dark powder
- Stability under test conditions: stable
- Storage condition of test material: at ambient temperature
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Water solubility (under test conditions): 2 mg/L and corresponding pH of 2,8 (with pure water)
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia Magna
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- Room temperature
- pH:
- 7.3 à 7.9 from T0 to T48h
- Dissolved oxygen:
- 7.6 mg/L to 8.3mg/L at T0
7.1 mg/L to 7.7 mg/L at T48H - Nominal and measured concentrations:
- Nominal concentration:
1.8 - 1.1 - 0.62 - 0.37 - 0.22 - 0.13 - 0.074 - 0.044 - 0.026 - 0.015 - 0.009 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer
- Type of flow-through : static
- No. of organisms per vessel: 5
- No. of vessels per concentration : 4
- No. of vessels per control (replicates): 4
TEST CONCENTRATIONS
- Range finding study: 1.8 - 0.7 - 0.2 - 0.07 - 0.02 - 0.007 - 0.02 mg/L
- Test concentrations: 1.8 - 1.1 - 0.62 - 0.37 - 0.22 - 0.13 - 0.074 - 0.044 - 0.026 - 0.015 - 0.009 mg/L - Reference substance (positive control):
- yes
- Remarks:
- K2Cr2O7
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.37 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI : 0.29-0.49
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.044 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI: 0.031-0.061
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.015 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- - Results with reference substance valid: K2Cr2O7
- EC50 (24h): =1.0 mg/L - Reported statistics and error estimates:
- EC 50 was calculated by using Log-Probit statistical model (software Toxcalc)
NOEC was calculated by using the Bonferroni t-test (software Toxcalc).
Any other information on results incl. tables
ASSAY:
T = 24 hours |
||||||||||||
Concen- tration (mg/L) |
1.8 |
1.1 |
0.62 |
0.37 |
0.22 |
0.13 |
0.074 |
0.044 |
0.026 |
0.015 |
0.009 |
Control |
I |
0 |
0 |
2 |
3 |
2 |
4 |
4 |
5 |
5 |
5 |
5 |
5 |
II |
0 |
3 |
2 |
3 |
2 |
4 |
4 |
5 |
5 |
5 |
5 |
5 |
III |
0 |
0 |
1 |
3 |
5 |
4 |
5 |
5 |
5 |
5 |
5 |
5 |
IV |
0 |
1 |
1 |
4 |
4 |
4 |
5 |
5 |
5 |
5 |
5 |
5 |
Total moving |
0 |
4 |
6 |
13 |
13 |
16 |
18 |
20 |
20 |
20 |
20 |
20 |
Immobili-zation rate % |
100% |
80% |
70% |
35% |
35% |
20% |
10% |
0% |
0% |
0% |
0% |
0% |
analytic assay: 2469
dissolved 02 at 1.8 mg/L: 7.3 mg/L
T = 48 hours |
|
|||||||||||
Concen- tration (mg/L) |
1.8 |
1.1 |
0.62 |
0.37 |
0.22 |
0.13 |
0.074 |
0.044 |
0.026 |
0.015 |
0.009 |
Control |
I |
0 |
0 |
0 |
2 |
1 |
1 |
1 |
1 |
4 |
3 |
5 |
5 |
II |
0 |
0 |
1 |
0 |
2 |
0 |
1 |
2 |
2 |
4 |
5 |
5 |
III |
0 |
0 |
0 |
1 |
0 |
2 |
1 |
2 |
2 |
4 |
5 |
5 |
IV |
0 |
0 |
0 |
0 |
1 |
2 |
3 |
2 |
2 |
5 |
5 |
5 |
Total moving |
0 |
0 |
1 |
3 |
4 |
5 |
6 |
7 |
10 |
16 |
20 |
20 |
Immobili-zation rate % |
100% |
100% |
95% |
85% |
80% |
75% |
70% |
65% |
50% |
20% |
0% |
0% |
analytical assay: 2469
dissolvedd 02 at 1.1 mg/L: 7.1 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the experimental conditions:
24-h EC50=0.37 mg/L (IC 95% : 0.29; 0.49)
48-h EC50=0.044 mg/L. (IC 95% : 0.031; 0.061)
48-h NOEC= 0.015 mg/mL - Executive summary:
The short-term toxicity (48 hours) on invertebrate (Daphnia magna) of the test item Sepisol Fast Violet 2B was assessed by determining the nominal effective concentration (EC 50%) at which 50 % of the test animals are immobilized. The method followed was the standard NF EN ISO 6341 under static condition.
EC50 (48h) values and associated confidence limits were calculated using a Log-Probit statistical model (software Toxcalc) and NOEC was worked out by using the Bonferroni t-test (software Toxcalc).
Test System: A stock solution (2 mg/mL) of the test substance was prepared in distilled water.
Immobilisations and viable abnormalities were recorded at 24 and 48 hours as well as pH and dissolved oxygen
Validity criteria were fulfilled and study was considered as valid..
A preliminary assay was performed at the following concentration: 1.8 - 0.7 - 0.2 - 0.07 - 0.02 - 0.007 mg/L
Test Levels: Test concentrations included control, 1.8 - 1.1 - 0.62 - 0.37 - 0.22 - 0.13 - 0.074 - 0.044 - 0.026 - 0.015 - 0.009 mg/L. Twenty daphnia were used per test group. 4 replicates were performed.
Conclusion:
24-h EC50=0.37 mg/L (IC 95%: 0.29 -0.49)
48-h EC50=0.044 mg/L (IC 95%: 0.031 -0.061)
48-h NOEC= 0.015 mg/mL
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