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EC number: 939-516-4
CAS number: -
In a preliminary study, no mortality was observed in rats exposed to a
saturated vapor for 1 hour and all animals died within 48 hours after
exposure to a saturated vapor for 2 hours.
In the L(Ct)50 experiments, compressed air at the rate of 500 ml/minute
was bubbled through glycerine which was heated to 200C. The rate of
vaporization of glycerine under these conditions, was gravimetrically
determined and calculated to be 11.0 mg/minute. The vapor-laden air was
diluted by adding make-up air at the rate of 500 ml/minute. The total
airflow (volume of air emerged from heated compound plus the volume of
make-up air added per unit time), entering the exposure chamber was 1
liter/minute. The nominal concentration of glycerine in the experimental
atmosphere was therefore 11.0 mg/L.
During the initial four hours of exposure, no significant adverse effect
was observed. However, after approximately 240 minutes, signs of mild
irritation, indicated by hyperemia of the eyelids and nasal discharges
were observed. These conditions became more severe as the exposure
progressed. The animals eventually salivated profusely and exhibited
dyspneic respiratory patterns. After approximately 360 minutes, all
animals began gasping for air and lay in prostration. Two animals died
at approximately the 419 minute timepoint. The third one died shortly
after the termination of the exposure. The fourth animal succumbed at
approximately 30 minutes post-exposure. The remaining animal succumbed
on the fourth post-exposure day. Based upon the time-to-death data, an
approximate Lt50 was graphically determined to be 423 minutes.
The L(Ct)50 was obtained by multiplying the nominal chamber
concentration, C, with the estimated Lt50 and was equal to 4655
Necropsies of the animals which succumbed revealed: 1) desquamation of
the epithelium of the trachea, 2) hyperemia and slight edema of the lung
tissues, and 3) extreme distention of the stomach and intestines with
air. No significant gross pathological condition was detected in other
organs or tissues.
The acute toxicity following 1 or 2 hours exposure to saturated vapors
of glycerine (generated by passing air through test material heated to
200C) was determined. Under the conditions of the study, the acute
inhalation exposure of rats for two hours to saturated vapors generated
at 200°C produced 100 per cent mortality, while for exposures of one
hour, no mortality was observed. The nominal concentration was 11.0 mg/L
and this study is of a condensation aerosol. Thus the 1 hour LC50 based
on nominal concentration was >11.0 mg/L. Under the OECD GHS guidelines,
a 4 hr LC50 can be determined from a 1 hour LC50 by dividing by 4. Thus
a calculated 4 hour LC50 value based on nominal concentration would be
In addition the L(Ct)50 was determined following exposure to 1100 mg/L.
The L(Ct)50 for Glycerine was 4655 mg minute/liter.
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