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EC number: 230-896-0 | CAS number: 7360-38-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 17 Jan - 4 Feb 1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- only four strains tested
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Propane-1,2,3-triyl 2-ethylhexanoate
- EC Number:
- 230-896-0
- EC Name:
- Propane-1,2,3-triyl 2-ethylhexanoate
- Cas Number:
- 7360-38-5
- Molecular formula:
- C27H50O6
- IUPAC Name:
- 1,3-bis[(2-ethylhexanoyl)oxy]propan-2-yl 2-ethylhexanoate
Constituent 1
Method
- Target gene:
- his operon
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- cofactor supplemented post-mitochondrial fraction (S9 mix), prepared from the livers of rats treated with Aroclor 1254
- Test concentrations with justification for top dose:
- Dose range finding test: 5, 50, 500 and 5000 µg/plate with and without metabolic activation
Main test: 50, 150, 500, 1500, 5000 µg/plate with and without metabolic activation - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 9-Aminoacridine (80 µg/pltae ) for TA1537; N-Ethyl-N'-nitrosoguanidine (3 µg/plate) for TA100, (5 µg/plate) for TA1535; 2-Nitrofluorene (1 µg/plate) for TA98.
- Remarks:
- Without metabolic activation
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: With S9: 2-Aminoanthracene (0.5 µg/plate) for TA98, (1 µg/plate) for TA100, (2 µg/plate) for TA1535 and TA1537
- Remarks:
- With metabolic activation
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
- Preincubation period: 1 h, 37 °C
- Exposure duration: 24 h (range finding test), 72 h (main test), 37 °C
NUMBER OF REPLICATIONS: triplicates, each in two experiments
DETERMINATION OF CYTOTOXICITY
- Method: relative total growth
OTHER:
- S9: added at 3% (main test) and 10% (range finding test and main test). In a rerun S9 of the main test S9 was added at 10% and 30%, respectively - Evaluation criteria:
- The test substance is considered to show evidence of mutagenic activity, if treatment with a test material produces an increase in revertant colony numbers of at least twice the concurrent solvent controls, with some evidence of a positive dose-relationship, in two separate experiments, with any bacterial strain either in the presence or absence of S9 mix.
The test substance is considered to show no evidence of mutagenic activity, if treatment with a test material does not produce reproducible increases of at least 1.5 times the concurrent solvent controls, at any dose level with any bacterial strain.
Results and discussion
Test results
- Key result
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: at 5000µg/plate for all strains in the presence and absence of S9 - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Results main experiment:
Experiment 1:
Dose Level (µg/plate) | Liver S-9 mix | TA 1535 | TA1537 | TA98 | TA100 | ||||
mean | SD | mean | SD | mean | SD | mean | SD | ||
5000 | - | 10 | 1.0 | 10 | 2.1 | 19 | 0.0 | 64 | 5.6 |
1500 | - | 11 | 1.7 | 9 | 0.6 | 23 | 3.0 | 90 | 1.0 |
500 | - | 14 | 2.5 | 10 | 2.1 | 20 | 1.5 | 96 | 2.5 |
150 | - | 12 | 2.0 | 9 | 1.5 | 22 | 3.1 | 102 | 2.6 |
50 | - | 11 | 1.0 | 9 | 0.6 | 21 | 2.0 | 101 | 3.1 |
0 | - | 17 | 1.0 | 10 | 0.6 | 24 | 5.0 | 104 | 4.5 |
Solvent | - | 15 | 1.0 | 10 | 1.0 | 26 | 4.0 | 112 | 2.5 |
ENNG (5 µg/plate) | - | 458 | 67.1 | ||||||
9-AC (80 µg/plate) | - | 1108 | 51.9 | ||||||
NF (1 µg/plate) | - | 85 | 9.0 | ||||||
ENNG (3 µg/plate) | - | 349 | 44.3 | ||||||
5000 | 3% | 12 | 1.5 | 10 | 0.6 | 24 | 2.1 | 72 | 1.5 |
1500 | 3% | 11 | 4.4 | 10 | 1.2 | 21 | 2.1 | 92 | 2.0 |
500 | 3% | 14 | 1.0 | 9 | 1.0 | 19 | 1.0 | 99 | 6.1 |
150 | 3% | 11 | 1.0 | 8 | 1.0 | 22 | 2.6 | 101 | 5.0 |
50 | 3% | 13 | 1.0 | 10 | 1.2 | 20 | 0.6 | 100 | 2.0 |
0 | 3% | 18 | 1.7 | 10 | 2.5 | 17 | 2.1 | 99 | 6.4 |
Solvent | 3% | 11 | 2.5 | 9 | 1.0 | 20 | 1.2 | 102 | 4.5 |
AA (0.5 µg/plate) | 3% | 161 | 20.0 | ||||||
AA (1 µg/plate) | 3% | 295 | 21.6 | ||||||
AA (2 µg/plate) | 3% | 56 | 3.8 | 80 | 11.5 | ||||
5000 | 10% | 12 | 1.5 | 10 | 2.1 | 22 | 1.5 | 73 | 1.0 |
1500 | 10% | 11 | 1.2 | 9 | 1.0 | 20 | 1.0 | 93 | 4.2 |
500 | 10% | 14 | 2.5 | 12 | 2.1 | 20 | 3.8 | 97 | 7.6 |
150 | 10% | 15 | 2.8 | 10 | 1.5 | 22 | 3.2 | 101 | 2.3 |
50 | 10% | 11 | 2.5 | 10 | 1.5 | 21 | 1.4 | 108 | 2.0 |
0 | 10% | 14 | 1.0 | 8 | 1.5 | 23 | 3.6 | 104 | 10.2 |
Solvent | 10% | 14 | 1.5 | 11 | 1.5 | 24 | 3.2 | 101 | 2.3 |
AA (0.5 µg/plate) | 10% | 143 | 11.0 | ||||||
AA (1 µg/plate) | 10% | 353 | 10.1 | ||||||
AA (2 µg/plate) | 10% | 57 | 7.6 | 70 | 8.0 |
Experiment 2:
Dose Level (µg/plate) | Liver S-9 mix | TA 1535 | TA1537 | TA98 | TA100 | ||||
mean | SD | mean | SD | mean | SD | mean | SD | ||
5000 | - | 10 | 3.2 | 10 | 1.0 | 19 | 3.1 | 82 | 6.1 |
1500 | - | 10 | 1.0 | 14 | 0.6 | 21 | 0.0 | 104 | 7.5 |
500 | - | 9 | 0.6 | 10 | 2.5 | 21 | 2.5 | 100 | 11.2 |
150 | - | 12 | 1.0 | 11 | 2.6 | 19 | 1.0 | 95 | 5.6 |
50 | - | 10 | 1.0 | 14 | 1.5 | 22 | 0.6 | 100 | 2.5 |
0 | - | 9 | 0.6 | 11 | 1.2 | 23 | 3.0 | 89 | 7.6 |
Solvent | - | 11 | 0.6 | 12 | 3.2 | 22 | 1.2 | 100 | 14.5 |
ENNG (5 µg/plate) | - | 439 | 48.8 | ||||||
9-AC (80 µg/plate) | - | x | x | ||||||
NF (1 µg/plate) | - | 80 | 8.0 | ||||||
ENNG (3 µg/plate) | - | 424 | 56.0 | ||||||
5000 | 10% | 11 | 2.0 | 17 | 3.0 | 19 | 2.1 | 92 | 4.0 |
1500 | 10% | 14 | 2.0 | 16 | 0.6 | 22 | 2.5 | 100 | 12.7 |
500 | 10% | 11 | 2.1 | 13 | 1.2 | 20 | 0.0 | 107 | 3.1 |
150 | 10% | 10 | 0.6 | 15 | 1.5 | 22 | 1.0 | 108 | 4.0 |
50 | 10% | 12 | 2.5 | 12 | 2.1 | 23 | 1.5 | 107 | 3.1 |
0 | 10% | 10 | 2.0 | 16 | 4.2 | 20 | 2.0 | 100 | 10.4 |
Solvent | 10% | 10 | 1.7 | 15 | 2.5 | 22 | 2.5 | 105 | 10.5 |
AA (0.5 µg/plate) | 10% | 87 | 1.2 | ||||||
AA (1 µg/plate) | 10% | 358 | 48.0 | ||||||
AA (2 µg/plate) | 10% | 71 | 18.8 | 68 | 12.8 | ||||
5000 | 30% | 9 | 0.6 | 17 | 1.2 | 19 | 3.5 | 101 | 7.6 |
1500 | 30% | 9 | 0.6 | 16 | 2.1 | 23 | 2.0 | 106 | 5.3 |
500 | 30% | 10 | 1.5 | 14 | 2.0 | 21 | 3.1 | 107 | 4.5 |
150 | 30% | 11 | 0.6 | 14 | 2.3 | 24 | 0.0 | 110 | 3.0 |
50 | 30% | 12 | 1.7 | 14 | 2.9 | 27 | 1.0 | 110 | 9.0 |
0 | 30% | 12 | 1.0 | 16 | 0.6 | 21 | 2.1 | 118 | 8.6 |
Solvent | 30% | 12 | 2.1 | 17 | 2.5 | 21 | 1.2 | 110 | 10.7 |
AA (0.5 µg/plate) | 30% | 83 | 6.1 | ||||||
AA (1 µg/plate) | 30% | 299 | 30.2 | ||||||
AA (2 µg/plate) | 30% | 74 | 9.3 | 61 | 8.5 |
SD: standard deviation
ENNG: N-ethyl-N'-nitro-N-nitrosoguanidine
9 -AC: 9 -aminoacridine
AA: 2 -aminoanthracene
NF: 2 -nitrofluorene
x: too many colonies to count accurately
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results: negative
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