Registration Dossier
Registration Dossier
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EC number: 700-728-5 | CAS number: 74805-60-0
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- (5Z)-5-(phenylmethylidene)imidazolidine-2,4-dione
- EC Number:
- 700-728-5
- Cas Number:
- 74805-60-0
- Molecular formula:
- C10H8N2O2
- IUPAC Name:
- (5Z)-5-(phenylmethylidene)imidazolidine-2,4-dione
- Test material form:
- solid: particulate/powder
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Suspended matter: 26.7 mg/L
- Dry matter: 4012 mg/L
- Colony forming units (CFU): 187 * 10^4 mL - Duration of test (contact time):
- 29 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 15.3 mg/L
- Based on:
- DOC
- Initial conc.:
- 24 mg/L
- Based on:
- test mat.
- Details on study design:
- TEST CONDITIONS
- Test temperature: 21.6-22.9°C (mean: 22.3°C)
TEST SYSTEM
Vessel: approximately 5000 mL Woulff Flasks with standard ground joints
Test batches: 2 vessels without test item and with inoculum (blanks)
1 vessel containing control item and inoculum
2 vessels containing test item and inoculum
- Measuring equipment: Carbon analyser (Manufacturer Shimadzu)
SAMPLING
- Sampling frequency: 0,4, 7, 14, 18, 22, 25, 28 and 29 days
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 0 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 2
- Sampling time:
- 4 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 1
- Sampling time:
- 14 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 41
- Sampling time:
- 22 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 92
- Sampling time:
- 29 d
- Details on results:
- Kinetic of test substance (in %):
= 2 after 4 day(s)
= 4 after 18 day(s)
= 92 after 29 day(s)
BOD5 / COD results
- Results with reference substance:
- Kinetic of control substance (in %):
= 70 after 4 day(s)
= 90 after 18 day(s)
= 97 after 29 day(s)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
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