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Diss Factsheets
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EC number: 480-410-8 | CAS number: 13482-23-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 14 Mar - 30 May 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- 1988
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Method: physico-chemical oxygen consumption
Controls: 3.5-Dichlorophenol, Purity 97%.
Test substance concentration: 1000, 1800, 3200, 5600, 10000 mg/L
Reference compound concentration: 2.5, 5, 10, 20 and 40 mg/L - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Source: aeration tank of a domestic sewage treatment plant (Municipal STP Cologne-Stammheim)
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2° C
- pH:
- 7.0 to 7.5
- Dissolved oxygen:
- 2.7 to 4.4 mg/L
- Nominal and measured concentrations:
- 1000; 1800; 3200; 5600; 10000 mg/L (nominal concentrations)
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichloronitrophenol, 2.5; 5; 10; 20; 40 mg/L
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The physico-chemical oxygen consumption control is carried out, since some substances may consume oxygen by chemical reactivity. In order to be able to differentiate between physico-chemical oxygen consumption and biological oxygen consumption(respiration), at least the maximum concentration of the test item is additionally tested without activated sludge.
- Results with reference substance (positive control):
- Inhibition of up to 96.7 % at 40 mg/L.
- Validity criteria fulfilled:
- yes
- Remarks:
- See 'Any other information on results incl. tables'.
- Conclusions:
- The test substance showed 45.8 % respiration inhibition of activated sludge at a test item concentration of 10000 mg/l.
- Executive summary:
A study was performed to assess the toxicity of 4-Methoxycyclohexanone to bacteria.
The study was in accordance with the Commission Directive 88/302/EEC; Official Journal of the EGL133PartC.ll (1988).
The activated sludge was exposed to 4-Methoxycyclohexanone at different concentrations.
The respiration rate of each mixture was determined after aeration periods of 3 hours.
4-Methoxycyclohexanone showed 45.8 % respiration inhibition of activated sludge at a test item concentration of 10000 mg/L.
The EC50 is higher than 10000 mg/L.
Reference
Validity criteria for the measurement of the bacterial toxicity:
Target condition according to guideline: | Actual condition according to the study: | Validity criteria met: |
In a 2004 international ring test organized by ISO using activated sludge derived from domestic sewage, the EC50 of 3,5-dichlorphenol was found to lie in the range 2 mg/L to 25 mg/L for total respiration. If the EC50 of 3,5-DCP does not lie in the expected range, the test should be repeated with activated sludge from another source. | The EC50 of the reference substance 3.5-dichlorophenol is in the range 5 - 30 mg/L. | Yes |
The test should be performed at a temperature within the range 20 ± 2°C. | The test was performed between 20 ± 2°C. | Yes |
The specific oxygen consumption of the controls shall be reported. | The oxygen consumption of the controls is stated. | Yes |
Two control respiration rates are within 15% of each other. | The respiratory rates of the 2 controls differ less than 15 %. | Yes |
Description of key information
The test substance showed 45.8 % respiration inhibition of activated sludge at a test item concentration of 10000 mg/l.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 10 000 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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