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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed OECD study with GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report date:
1994

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
3-nitro-p-toluic acid
EC Number:
202-549-3
EC Name:
3-nitro-p-toluic acid
Cas Number:
96-98-0
Molecular formula:
C8H7NO4
IUPAC Name:
4-methyl-3-nitrobenzoic acid
Details on test material:
purity: 99.4 %

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: zebra fish; Brachydanio rerio (HAMILTON-BUCHANAN)
- Source: Corporate Toxicology (Hoechst AG)
- Age at study initiation: approximately two months
- Length at study initiation: 2.8 - 3.3 cm
- Feeding during test: no

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions: same as test or not
- Type and amount of food: Tetra Min, Tetra Werke, Melle
- Feeding frequency: twice daily ad libitum

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Hardness:
2.1 mmol Ca 2+ Mg 2+ / L
Test temperature:
21.6 - 22.2 °C
pH:
7.5 - 7.7
Dissolved oxygen:
7.6 - 9.5 mg/L
Nominal and measured concentrations:
nominal: 100 mg/L
measured (average): 99.1 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: glass, calibrated to 10 liters
- Material, size, headspace, fill volume: 30 cm x 22 cm x 24 cm; Hostalit Z
- Aeration: no
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates):1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water, composition according to ISO/DIS 7346/1

OTHER TEST CONDITIONS
- Photoperiod: 12 hours dark / 12 hours light
- Light intensity: 700 lux

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
0 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: control
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
No changes in appearance and behavior
No mortality

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Based on the test result the LC50 is greater than 100 mg/L concerning the acute toxicity to fish.
Executive summary:

The substance was tested for acute toxicity to fish according to OECD guideline 203. One concentration (100 mg/L) was tested over a period of 96 hours.

No lethality occurred and the fish showed no changes in appearance and behavior in comparison to the control group (0 mg/L).