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EC number: 444-960-2 | CAS number: 39148-16-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21 Mar 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- other: Commission Directive 87/302/EEC: "Activated Sludge Respiration Inhibition Test", Official Journal of the European Communities No. L133/118-122, dated May 30, 1988.
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Hessisches Ministerium für Umwelt, Landwirtschaft und Forsten
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- 4545 mg test item (content of sodium ethyl phosphonate: 22.1% w/w) were filled up to 200 mL with deionized water to achieve a concentration of nominal 5 g/L of the active ingredient sodium ethyl phosphonate.
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Origin: supplied by the sewage plant GroB-Zimmern, Germany
- Pretreatment: The sludge was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was resuspended in tap water and again centrifuged. The latter procedure was repeated twice. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge (g) to its dry weight (g) determined. Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 3g dry material
per litre, were made up with tap water. The pH of the activated sludge was determined to be pH 7.8. To this mixture, 50 mL synthetic sewage feed per litre was added daily, starting one day prior to use, and the sludge was kept at room temperature under continuous aeration until use. Immediately before use, the dry weight of the activated sludge was determined, and diluted to 3g/L with tap water. The pH of the activated sludge was determined to be 7.8. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 19 °C
- pH:
- 7.3 - 8.4
- Dissolved oxygen:
- 6.8 - 7.3 mg O2/L
- Nominal and measured concentrations:
- control, 10, 32, 100, 320, and 1000 mg/L (active ingredient: AE C529354, nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: glass flasks of approximately 1 litre volume filled with 500 mL test solution
- Aeration: with compressed air (approximately 0.6 litre per minute)
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Intervals of water quality measurement: The pH and the oxygen concentrations were determined at the start and at the end of the incubation period in all treatments. The water temperature was measured in one control medium at the start and the end of the incubation period. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: equivalent to EC50 >4524 mg AEC529354 technical concentrate 22.1%/L
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: equivalent to EC50 >4524 mg AEC529354 technical concentrate 22.1%/L
- Details on results:
- All results are based on concentrations of the active ingredient sodium ethyl phosphonate (content of 22.1% w/w in test item). In comparison to the inoculum controls the respiration rate of the activated sludge was not or only slightly inhibited by 0.4% to 7.1% up to the highest tested concentration of 1000 mg sodium ethyl phosphonate/L. Concentrations exceeding 1000 mg sodium ethyl phosphonate/L nominal were not tested.
- Results with reference substance (positive control):
- EC50 (3h) = 6.8 mg/L (95% CL: 4.4 - 9.5 mg/L)
- Validity criteria fulfilled:
- yes
- Remarks:
- see Table 2 in 'any other information on results incl. tables'
Reference
Table 1: Influence of AE C529354; technical concentrate,based on active ingredient sodium ethyl phosphonate, on oxygen consumption of activated sludge
Flask |
Treatment |
Concentration of a.i. [mg/L] |
Oxygen consumption [mg O2/L min] |
Inhibition % |
pH-values |
|
Oxygen concentration [mg O2/L] |
|
|
|
|
|
|
start |
end |
start |
end |
1 |
Control |
- |
0.440 |
- |
7.8 |
8.4 |
6.9 |
7.3 |
10 |
Control |
- |
0.463 |
- |
7.7 |
8.4 |
7.0 |
6.8 |
Mean |
|
|
0.452 |
|
|
|
|
|
% deviation |
|
|
5 |
|
|
|
|
|
5 |
Test item |
1000 |
0.450 |
0.4 |
7.3 |
8.3 |
6.9 |
7.2 |
6 |
Test item |
320 |
0.440 |
2.7 |
7.3 |
8.3 |
7.0 |
7.3 |
7 |
Test item |
100 |
0.450 |
0.4 |
7.3 |
8.3 |
7.0 |
6.9 |
8 |
Test item |
32 |
0.420 |
7.1 |
7.4 |
8.4 |
6.9 |
7.1 |
9 |
Test item |
10 |
0.430 |
4.9 |
7.4 |
8.4 |
7.0 |
6.8 |
Table 2: Validity criteria for OECD 209.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The blank controls (without the test substance or reference substance) oxygen uptake rate should not be less than 20 mg oxygen per one gram of activated sludge (dry weight of suspended solids) in an hour. |
The blank controls oxygen uptake rate was 22 and 23.15 mg O2 per gram dry weight of suspended solids in an hour |
Yes |
The coefficient of variation of oxygen uptake rate in control replicates should not be more than 30% at the end of definitive test. |
The coefficient of variation of oxygen uptake rate in control replicates was 3.6% |
Yes |
Description of key information
EC20 and EC50 (3 h) > 1000 mg a.i. (Fosetyl-Sodium)/L for activated sludge (OECD 209) (equivalent to >4524 mg Fosetyl-Sodium technical concentrate 22.1%/L)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
A GLP study according to OECD 209 on toxicity to microorganisms was performed with Fosetyl-sodium (technical concentrate; 22.1%; Code: AE C529354 00 TK22 A101, containing 22.1% w/w Fosetyl-Sodium) under static conditions.
The study was designed to assess the toxicity of the test item to bacteria. The method assesses the effect on microorganisms by measuring the respiration rate under defined conditions in the presence of different concentrations of the test item. Mixed population of aquatic microorganisms (activated sludge) were exposed to Fosetyl-Sodium; technical concentrate at nominal concentrations of 10, 32, 100, 320 and 1000 mg test item/L. The respiration rate of each mixture was measured after an aerated incubation period of 3 hours. Two controls without test item were included in the test design, one at the start and the other at the end of the test series. Each batch of activated sludge is checked using 3.5-dichlorophenol as reference substance (tested rates: 3.2, 10 and 32 mg/L).
All results are based on concentrations of the active ingredient sodium ethyl phosphonate (content of 22.1 % w/w in test item). In comparison to the inoculum controls the respiration rate of the activated sludge was not or only slightly inhibited by 0.4% to 7.1% up to the highest tested concentration of 1000 mg/L. Based on measured inhibition rates, the 3-hour EC20 and EC50 could not be quantified because up to the highest nominal test concentration of 1000 mg sodium ethyl phosphonate/L less than 20% inhibition was noted. Nevertheless, the 3-hour EC20 and EC50 are clearly higher than 1000 mg sodium ethyl phosphonate/L (equivalent to > 4524 mg Fosetyl-Sodium technical concentrate 22.1%/L) under the present test conditions.
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