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EC number: 216-629-0 | CAS number: 1630-78-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experimental starting date: June 22 2020 Experimental completion date: June 26 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: "Fish, Acute Toxicity Test" stipulated in the "Testing Methods for New Chemical Substances" of Japanese Chemical substances Control Law .
- Version / remarks:
- Latest revision July 1, 2019
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Chemical name; (E)-1,2,-Difluoroethylene
Other name: TKN1
Purity of test item: 99.9989% (GC area)
Colourless gas
Flammable, stable in dark storage space at room temperature - Analytical monitoring:
- yes
- Details on sampling:
- The test solution for analysis (approximately 10 mL - all test levels) was taken out from the middle layer of the test vessel.
- Vehicle:
- no
- Details on test solutions:
- Preparation of test solution
After approximately 2 L of dilution water was added in 3 L sampling bag for gas analysis (Tedlar bag), about 500 mL gaseous test sample (approximately 10 minutes at about 50 mL/min) was enclosed in the bag. It was then shaken overnight to prepare the stock solution. After shaking, the test item concentration in the stock solution was measured. As a result, the stock solutions of 649 and 1080 mg/L were prepared at the start of exposure and before renewal at the 48 hours, respectively. Required volumes of the stock solution and dilution water were mixed in the test container, and stirred to prepare the test solution of 300 mg/L. The stock solution and the test solution were prepared at the start of exposure and the renewal after 48 hours. - Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- supplier: CERI Kurume (in-test facility production)
Total length: 2.0 +/- 1.0 cm
Allocation: random sampling
Acclimation
Age at the start of exposure: 1 month
Water: Dechlorinated tap water
Dissolved oxygen concentration: At least 80% of air saturation value
Temperature: 24 +/1 1°C
Lighting condition: Room light - 16-hour light/ 8-hour dark
Feed: Newly hatched Artemia nauplii
Feeding amount and frequnecy: Saturation volume, every daya
No use of medicant for external disnfection
Duration of acclimation: 17 days
Moratlity of test group: 0%
Feed withdrawal: 24 hours before the start of exposure - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 40 mg/L of CaCO3
- Test temperature:
- 23°
- pH:
- pH meter HM-21P (DKK-TOA)
7.9 - Dissolved oxygen:
- Instrument: Dissolved oxygen meter HQ30d
7.9 mg/L at start of study
6.7 mg/L at end of study - Salinity:
- Not applicable as freshwater study
- Conductivity:
- 17 mS/m
- Nominal and measured concentrations:
- 300 mg/L (nominal concentration) as limit test
138 mg/L as measured concentration
The test concentration was derived from the results of the preliminary study. - Details on test conditions:
- Type of test: Semi-static regime (renewal whole test solution at 48 hours after the start of exposure), closed system with headspace
Test vessel: 3 L glass closed tank
Cover on test vessel: Airtight transparent plastic lid
Replicate: 1 replicate/test vessel
Number of fish per test vessel: 7
Volume of test solution: approximately 2.7 L per test vessel.
Aeration: No aeration
pH adjustment: No pH adjustment
Lighting condition: Room light, 16-hr light/8-hour dark
Feeding: no feeding
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:Dechlorinated tap water
- Total organic carbon: <0.5 mg/L
- Particulate matter: <1 mg/L
- Chlorine: <0.02 mgL
- Alkalinity: 41 mg/L CaCO3
Obsevation and measurement
a) Mortality and visible abnormality were observed at 3, 24, 48, 72 and 96 hours after the start of exposure.
b) Total length and body weight of test organism
c) Appearance of test solution
d) Condition of test solution
e) Concentration of test item in test solution - Reference substance (positive control):
- yes
- Remarks:
- Copper (II) sulphate pentahydrate
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 138 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- Mortality
Number of dead fish in the control at the end of exposure was 0.
Number of dead fish at the end of the exposure was zero.
Observed performance status
No abnormal response was observed in the control
No abnormal response in the exposure level during exposure was observed.
Size of test organism
Total length: 2.0 +/- 0.1 cm 91.9 - 2.2 cm)
Body weight: 0.074 +/- 0.009 (0.067 - 0.092 g)
Observation of test solution:
The test solutions in all test levels were colorless and clear at the start and before the renewal.
Conditions of test solution:
The measured values of dissolved oxygen concentration, pH and temperature during exposure were 6.6 - 8.4 mg/L, 7.1 -7.6 and 24.0 - 24.5°C.
The measured values of dissolved oxygen concentration met the criterion for the validity of the test (at least 60% of saturate concentration at the water temperature).
Concentration of test item in test solution: The measured concentrations of the test item in the test solutions were 267 and 176 mg/L at the start of exposure and after the renewal, and 92.9 and 65.1 mg/L before the renewal and at the end of exposure were 34.8 and 37.0% of the concentrations at preparation. The time-weighted mean of the measured concentrations was 138 mg/L. - Results with reference substance (positive control):
- A 96-hour acute toxicity test with reference substances is periodically conducted. The latest test with Copper (II) sulfate pentahydrate gave a result of 96-hr LC50 0.43 mg/L. This value was within the stipulated range of the test facility.
- Sublethal observations / clinical signs:
Cumulative Morality
Measured concentration (mg/L)*
Cumulative mortality (%)
3 hours
24 hours
48 hours
72 hours
96 hours
Control
0
0
0
0
0
138
0
0
0
0
0
*Time-weighted mean of the measured concentrations
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96-hour LC50 of the test item for Medaka was >138 mg/L.
- Executive summary:
The objective of the study was to determine the 96 -hr LC50 by conducting an acute toxicity study of TKN1 in Medaka.The test guideline followed was OECD Guidelines for Testing of Chemicals, No. 203, July 17, 1992, "Acute fish Toxicity Test".
Seven fish were exposed to 300 mg/L nominal concentration (148 mg/L measured concentration) for a period of 96 hours under semi-static conditions
No effects were observed on the test organisms, and the time-weighted mean of the measured concentrations during the exposure period was 138 mg/L, so the 96 -hour LC50 was > 138 mg/L. The environmental conditions were within the suitable range. Therefore, it is concluded that this study complied with the applied test guidelines.
Reference
Description of key information
The objective of the study was to determine the 96 -hr LC50 by conducting an acute toxicity study of TKN1 in Medaka.The test guideline followed was OECD Guidelines for Testing of Chemicals, No. 203, July 17, 1992, "Acute fish Toxicity Test".
Seven fish were exposed to 300 mg/L nominal concentration (148 mg/L measured concentration) for a period of 96 hours under semi-static conditions (renewal at 48 hrs) in a closed system with headspace.
No effects were observed on the test organisms, and the time-weighted mean of the measured concentrations during the exposure period was 138 mg/L, so the 96 -hour LC50 was > 138 mg/L.
The environmental conditions were within the suitable range. Therefore, it is concluded that this study complied with the applied test guidelines.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- > 138 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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