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EC number: 675-808-5 | CAS number: 18611-43-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 21.4. - 12.7.2021
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 021
- Report date:
- 2021
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- 2019,2020
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1,1,3,3-tetrachloroprop-1-ene
- EC Number:
- 675-808-5
- Cas Number:
- 18611-43-3
- Molecular formula:
- C3H2Cl4
- IUPAC Name:
- 1,1,3,3-tetrachloroprop-1-ene
- Test material form:
- liquid
- Details on test material:
- concentration 99,87%
Constituent 1
Method
- Target gene:
- he experiments were carried out using histidine-requiring auxotroph strains of Salmonella
typhimurium (Salmonella typhimurium TA98, TA100, TA1535 and TA1537), and the
tryptophan-requiring auxotroph strain of Escherichia coli (Escherichia coli WP2 uvrA) in
the presence and absence of a post mitochondrial supernatant (S9) prepared from livers of
Phenobarbital/β-naphthoflavone-induced rats.
- Metabolic activation:
- with
- Metabolic activation system:
- The experiments were carried out using histidine-requiring auxotroph strains of Salmonella
typhimurium (Salmonella typhimurium TA98, TA100, TA1535 and TA1537), and the
tryptophan-requiring auxotroph strain of Escherichia coli (Escherichia coli WP2 uvrA) in
the presence and absence of a post mitochondrial supernatant (S9) prepared from livers of
Phenobarbital/β-naphthoflavone-induced rats. - Test concentrations with justification for top dose:
- in the case of Salmonella typhimurium strains:
-S9: 1000, 500, 160, 50, 16 and 5 μg/plate,
+S9: 1600, 500, 160, 50, 16 and 5 μg/plate;
in the case of Escherichia coli WP2 uvrA:
±S9: 1600, 500, 160, 50, 16 and 5 μg/plate. - Vehicle / solvent:
- dimethyl sulfoxide
Controls
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Dimethyl sulfoxide (DMSO)
- True negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other:
- Details on test system and experimental conditions:
- The study includes a preliminary solubility test, a preliminary concentration range finding
test (informatory toxicity test) an initial mutation test (plate incorporation test) and a
confirmatory mutation test (pre-incubation test).
In the preliminary concentration range finding test as well as in the initial mutation test the
plate incorporation method was used.
A standard plate incorporation procedure was performed as an initial mutation test. Bacteria
(cultured in Nutrient Broth No.2. (Section: 5.4.2)) were exposed to the test item both in the
presence and absence of rat liver S9 as metabolic activation system.
Molten top agar was prepared and kept at 45°C. Two mL of top agar was aliquoted into
individual test tubes (3 tubes per controls or concentration level). The equivalent number of
minimal glucose agar plates was properly labelled. Conditions were investigated in triplicate.
The test item and other components were prepared fresh and added to the overlay (45°C). - Evaluation criteria:
- The colony numbers on the untreated, vehicle control, positive control and the test plates
were determined visually by manual counting and the mean values, standard deviations and
the mutation rates were calculated.
An increase is considered biologically relevant if:
- in strain Salmonella typhimurium TA100 the number of reversions is at least twice as high
as the reversion rate of the vehicle control,
- in strain Salmonella typhimurium TA98, TA1535, TA1537 and Escherichia coli WP2
uvrA the number of reversions is at least three times higher than the reversion rate of the
vehicle control. - Statistics:
- No precipitation of the test item was observed on the plates after about 48 hours incubation
in the examined bacterial strains at any examined concentration level (±S9) in the performed
main experiments.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Remarks:
- In general, 160 μg/plate in absence exogenous metabolic activation was considered as the lowest concentration showing unequivocal cytotoxicity.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Remarks:
- In general, 160 μg/plate in absence (−S9), exogenous metabolic activation (+S9)) was considered as the lowest concentration showing unequivocal cytotoxicity.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Remarks:
- In general, 160 μg/plate in the presence of exogenous metabolic activation (+S9)) was considered as the lowest concentration showing unequivocal cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- The reported data of this mutagenicity assay show that under the experimental conditions
applied, the test item did not induce gene mutations by base pair changes or frameshifts
in the genome of the strains used. - Remarks on result:
- no mutagenic potential (based on QSAR/QSPR prediction)
Applicant's summary and conclusion
- Conclusions:
- The reported data of this mutagenicity assay show that under the experimental conditions
applied, the test item did not induce gene mutations by base pair changes or frameshifts
in the genome of the strains used.
In conclusion, the test item 1133-Tetrachloropropene has no mutagenic activity on the
applied bacterium tester strains under the test conditions used in this study. - Executive summary:
The test item 1133-Tetrachloropropene has no mutagenic activity on the
applied bacterium tester strains under the test conditions used in this study
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