4-(aminomethyl)octane-1,8-diamine

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2018.09.06

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source (i.e. manufacturer or supplier) and lot/batch number of test material:
Manufacturer: Ascend Performance Materials Inc.
Lot no.: 1205635
- Purity, including information on contaminants, isomers, etc.:
100%



STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
Ambient
- Stability and homogeneity of the test material in the vehicle/solvent under test conditions (e.g. in the exposure medium) and during storage:
stable
- Stability in the medium, i.e. sensitivity of the test material to hydrolysis and/or photolysis:
stable
- Solubility and stability of the test material in the solvent/vehicle and the exposure medium:
highly soluble
- Reactivity of the test material with the incubation material used (e.g. plastic ware):
non-reactive

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing (e.g. warming, grinding):
none
- Preliminary purification step (if any):
none

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):
Brazos Valley River Sewer South

Duration of test (contact time):

28 d

Initial conc.:

2 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

This study was conducted to measure the biodegradability of the test substance, Hexatran-100, in an aerobic, aqueous medium at a concentration of approximately 2 mg/L of the test substance, as received. The solutions were prepared using inoculated mineral media with secondary effluent. The solutions were kept in closed bottles in the dark at 20°C. The degradation was measured by dissolved oxygen analyses over a 28-day period. A standard reagent of known performance (distilled aniline) and a blank were tested at the same time to serve as a control for biological activity.

Reference substance:

aniline

Parameter:

% degradation (O2 consumption)

Value:

-10.2

Sampling time:

28 d

Parameter:

% degradation (O2 consumption)

Value:

-2.9

Sampling time:

21 d

Parameter:

% degradation (O2 consumption)

Value:

2.5

Sampling time:

14 d

Parameter:

% degradation (O2 consumption)

Value:

-4.3

Sampling time:

7 d

Results with reference substance:

day 7: 83.5%
day 14: 90.0%
day 21: 96.4%
day28: 86.2%

The dissolved oxygen (DO) readings and biodegradation calculations for the blank, the distilled aniline reference standard, and for the test substance are presented in Tables 1 and 2. The toxicity reference standard showed sufficient oxygen depletion, indicating that the test substance and reference materials were not toxic to the microbes in the activated sludge.

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

Test substance is considered not biodegradable at 2 mg/L

Executive summary:

For test substance, Hexatran-100, at 2 mg/L, values of -4.3%, 2.5%, -2.9% and -10.2% biodegradation was obtained on Days 7, 14, 21 and 28; therefore, the test substance is considered not biodegradable at 2 mg/L.

Key value for chemical safety assessment

Biodegradation in water:

not biodegradable

Type of water:

freshwater