Ethoprophos

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1977-04-26 to 1977-04-27

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1977

Report date:

1977

Materials and methods

Test guideline

Test guideline 1

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Deviations:

yes

Remarks:

test item applied to abraded and non-abraded skin

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: CFR section 1500.41

Deviations:

no

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Ethoprophos

EC Number:

236-152-1

EC Name:

Ethoprophos

Cas Number:

13194-48-4

Molecular formula:

C8H19O2PS2

IUPAC Name:

ethyl bis(propylsulfanyl)phosphinate

Test material form:

liquid

Test animals

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: not specified
- Age at study initiation: not specified
- Weight at study initiation: 2.0 - 2.5 kg
- Housing: individually, no further specification
- Diet (ad libitum): not specified
- Water (ad libitum): yes, except 24 h exposure period
- Acclimation period: not specified

ENVIRONMENTAL CONDITIONS
not specified

Test system

Type of coverage:

occlusive

Preparation of test site:

other: clipped and abraded

Remarks:

clipped before

Vehicle:

unchanged (no vehicle)

Controls:

no

Amount / concentration applied:

TEST MATERIAL
- Amount applied: 0.5 mL

Duration of treatment / exposure:

24 h

Observation period:

72 h

Number of animals:

6 animals

Details on study design:

TEST SITE
- Area of exposure: dorsum
- Type of wrap: inch gauze patch held in place with Dermicel tape. The trunks of the animals were wrapped with rubberized cloth.

REMOVAL OF TEST SUBSTANCE
not specified

OBSERVATION TIME POINTS
24 and 48 h

SCORING SYSTEM: Scoring of erythema, eschar and edema for calculation of primary irritation index

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

edema score

Basis:

mean

Time point:

24/48/72 h

Max. score:

4

Remarks on result:

not determinable

Remarks:

due to acute toxicity (mortality) within 8 h

Results 2

Irritation parameter:

erythema score

Basis:

mean

Time point:

24/48/72 h

Max. score:

4

Remarks on result:

not determinable

Remarks:

due to acute toxicity (mortality) within 8 h

Irritant / corrosive response data:

Dermal irritation could not be determined in this study.

Other effects:

- Other adverse local effects:
All animals experienced salivation, loss of righting reflex, tremors and mortality within the first 8 hours.

Applicant's summary and conclusion

Interpretation of results:

study cannot be used for classification

Conclusions:

Under the test condition, the test item was not irritating to the skin of the rabbit. Nevertheless all animals died before the end of the observation period due to the high dermal toxicity of the test item in the rabbit. Due to the high acute dermal toxicity of the test item to the rabbit, the primary skin irritating property was not further investigated.

Executive summary:

In a 1977 study similar to OECD 404, 0.5 mL undiluted test substance was applied under occlusive conditions to the intact or abraded shorn dorsal skin of 6 male New Zealand White rabbits for 24 hours.

Signs of toxicity (salivation, loss of righting reflex and tremors) occurred within 8 hours of administration and all animals died within 24 hours. Dermal irritation could not be determined in this study.

Under the test condition, the test item was not irritating to the skin of the rabbit. Nevertheless all animals died before the end of the observation period due to the high dermal toxicity of the test item in the rabbit. Due to the high acute dermal toxicity of the test item to the rabbit, the primary skin irritating property was not further investigated.