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EC number: 260-300-4 | CAS number: 56634-95-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic plants other than algae
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic plants other than algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22 Oct - 08 Nov, 1993
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: EPA OPP 122-2 and 123-2
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- At test initiation (day 0) and test termination (day 14), the concentration of test substance was determined in a single sample from each of the test and control solutions. Samples analyzed at 0 hour were removed from the test solution remaining in the 500-mL volumetric flasks subsequent to division into the replicate test vessels. At test termination, the replicate exposure solutions for each treatment level and control solutions were individually composited. Samples were then removed from the composite solution of each treatment level and control for analysis.
- Vehicle:
- yes
- Remarks:
- Acetone
- Details on test solutions:
- A 10 mg a.i./mL primary stock solution was prepared by diluting 0.5274 g (0.5000 g a.s.) of test material in a volumetric flask to volume with 50 mL of acetone.
Secondary stock solutions with nominal concentrations of 0.38, 0.75,1.5, 3.0 and 6.0 mg a.i./mL were then prepared by diluting appropriate volumes of the 10 mg a.i./mL primary stock solution with acetone to a volume of 10 mL. Test solutions with nominal concentrations of 0.038, 0.075, 0.15, 0.30 and 0.60 mg a.i./L were prepared by diluting 50 µL of the appropriate stock solution with Hoagland's medium to a volume of 500 mL. No visible signs of undissolved test material was observed in any of the test solutions. - Test organisms (species):
- Lemna gibba
- Details on test organisms:
- The duckweed Lemna gibba, strain G3, was obtained from the University of California and maintained in stock culture at the laboratory.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 14 d
- Test temperature:
- 25 °C
- pH:
- 5.1 - 6.3
- Nominal and measured concentrations:
- Nominal concentrations: 0.038, 0.075, 0.15, 0.30 and 0.60 mg a.i./L
Initial measured concentration: 0.041, 0.077, 0.12, 0.25 and 0.40 mg a.i./L - Details on test conditions:
- Sterile 270-mL crystallizing dishes, three per treatment level and the controls, were conditioned prior to use by rinsing with the appropriate solution. 100 mL of the test solution was then placed in each of the three replicate vessels. Each test vessel was covered with an inverted, sterile, glass petri dish.
Approximately 15 minutes after the test solutions were prepared and added to the test vessels, an inoculum of five plants with three fronds each was aseptically introduced into each vessel. Test vessels were then randomly placed, based on computer-generated random numbers, on a single shelf within an environmental chamber.
The test was conducted in an environmental chamber designed to maintain the following conditions: a temperature of 25 ± 1 °C and continuous lighting with a light intensity within the range of 350 to 500 footcandles (3800 to 5400 lux).
At each 3-day interval (i.e., day 3, 6, 9 and 12) and at test termination (day 14), fronds were counted and observations were made. - Key result
- Duration:
- 14 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.21 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- act. ingr.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95 % C.I.: 0.14 - 0.31 mg a.i./L
- Key result
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.077 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- act. ingr.
- Basis for effect:
- biomass
- frond number
- Key result
- Duration:
- 14 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.27 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- act. ingr.
- Basis for effect:
- frond number
- Remarks on result:
- other: 95 % C.I.: 0.21 - 0.34 mg a.i./L
- Details on results:
- At test termination, fronds exposed to the three highest treatment levels (0.12, 0.25 and 0.40 mg a.i./L) were observed to have very little root formation and to be smaller and slightly chlorotic compared to the control.
Fronds exposed to the 0.041 and 0.077 mg a.i./L treatment levels were observed to be slightly chlorotic compared to the control.
Because of the observed decrease in test material concentration between test initiation and test termination (measured concentrations in most exposure solutions were generally -below the established limit of quantitation), the results of this test are based on initial measured concentrations. - Validity criteria fulfilled:
- not specified
- Conclusions:
- The present guideline study was conducted in compliance with GLP. Under the test conditions used, the 14-day EC50 for Lemna gibba was 0.21 mg a.i./L. The NOEC was 0.077 mg a.i./L.
Reference
Please refer to "overall remark/ attached background material" field for result tables.
Description of key information
From the key study, the 14-d EC50 for aquatic plants exposed to the present test material was 0.21 mg a.i./L and 14-d NOEC 0.077 mg a.i./L in freshwater.
Key value for chemical safety assessment
- EC50 for freshwater plants:
- 0.21 mg/L
- EC10 or NOEC for freshwater plants:
- 0.077 mg/L
Additional information
One GLP-study is available for toxicity to aquatic plants other than algae and was identified as key study.
In key study (1993), the acute toxicity of test material to Lemna gibba was determined in a 14-day static test according to EPA OPP 122-2 and 123-2 guidelines.The 14-day ER50and NOEC of the test substance were determined to be 0.21 mg a.s./L and 0.077 mg a.i./L, respectively, based on the mean initial measured concentrations.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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