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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 19 January 2022
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 022
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Enzymatically produced steviol glycosides
- IUPAC Name:
- Enzymatically produced steviol glycosides
- Test material form:
- solid: particulate/powder
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Remarks:
- The inoculum was used as an activated sludge from the aeration tank of a Sewage treatment plant treating predominantly domestic sewage from Sewage (Waste water) Treatment Plant.
- Details on inoculum:
- A fresh sample of activated sludge was collected from the aeration tank of a Sewage treatment plant treating predominantly domestic sewage from Sewage (Wastewater) Treatment Plant.
Removed the coarse particles and centrifuged for 10 minutes at 3169 rpm (1100 g). The supernatant was discarded.
The sludge was washed in the mineral medium and suspended the concentrated sludge of 18.8864 g in mineral medium to yield a concentration of approximately 3 to 5 g of suspended solids/Litre.
Centrifuged the re-suspended sludge at 3169 rpm (1100 g) for 10 minutes after thorough mixing and discarded the supernatant and again re-suspended the washed sludge in a 1000 mL volume of mineral medium sludge and repeated this procedure once again and the sludge was found to be free from excess substrate and aerobic thereafter until inoculation. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 179.6 mg/L
- Based on:
- TOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
Reference substance
- Reference substance:
- acetic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 69.47
- Sampling time:
- 28 d
- Details on results:
- The results of the CO2 evolution test indicated that the test item has reached a maximum 69.47% biodegradability by day 28. The test item has reached >60% of the ThCO2 (degradation) by day 28. Since, the test item is an UVCB substance containing multi-constituents which accounted for 100% of total composition. Hence, 10-day window criterion should not be considered as a requirement for assuring the desired stringency for multi-component substances.
The test item UVCB substances exceeded the 60% pass level within the standard test duration of 28 days are considered as readily biodegradable substances.
The difference of extremes of replicate values of the removal of the test item at the end of the test is 3.64% which is less than 20%. Therefore, the test was considered valid.
In the toxicity test, which includes both the test substance and the reference substance (sodium acetate) in the same flask, the substances yielded 14.87% biodegradability by day 14 which is less than 25% biodegradation within 14 days. Hence, the test item is regarded as inhibitory to microorganisms in the inoculum.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The Assessment of Ready Biodegradability of test item Enzymatically produced steviol glycosides is “Readily Biodegradable” and “Inhibitory to microorganisms in the inoculum” under the conditions of the CO2 Evolution test according to OECD criteria.
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