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EC number: 242-440-8 | CAS number: 18599-22-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- March 1977
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- significant methodological deficiencies
- Remarks:
- The study was performed pre-GLP and pre-OECD Test Guidelines. It was performed by an experienced reputable laboratory. Limited information is provided on the method used.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 977
- Report date:
- 1977
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- - Principle of test: Analysis of mutagenic activity of 1-Butene, 4-bromo-3,3,4,4-tetrafluoro- in the Salmonella/microsome assay.
- Short description of test conditions: Five histidine-requiring strains of Salomonella typhimurim were sued in the assays. Strains 1535 and TA 100 were used in the detection of base-pair substitution mutations, with strains TA 1537, TA 1538 and TA 98 used ti detect frame-shift mutations. The assays were performed in the presence and absence of a ra-liver homogenate activation system (S.9) . - GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 4-bromo-3,3,4,4-tetrafluorobut-1-ene
- EC Number:
- 242-440-8
- EC Name:
- 4-bromo-3,3,4,4-tetrafluorobut-1-ene
- Cas Number:
- 18599-22-9
- Molecular formula:
- C4H3BrF4
- IUPAC Name:
- 4-bromo-3,3,4,4-tetrafluorobut-1-ene
- Test material form:
- not specified
1
Method
- Target gene:
- Not stated
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 98
- Details on mammalian cell type (if applicable):
- Not stated
- Additional strain / cell type characteristics:
- not specified
- Species / strain / cell type:
- S. typhimurium TA 1538
- Details on mammalian cell type (if applicable):
- Not stated
- Additional strain / cell type characteristics:
- not specified
- Species / strain / cell type:
- S. typhimurium TA 1537
- Details on mammalian cell type (if applicable):
- Not stated
- Additional strain / cell type characteristics:
- not specified
- Species / strain / cell type:
- S. typhimurium TA 100
- Details on mammalian cell type (if applicable):
- Not stated
- Additional strain / cell type characteristics:
- not specified
- Species / strain / cell type:
- S. typhimurium TA 1535
- Details on mammalian cell type (if applicable):
- Not stated
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- Type and composition of metabolic activation system:
- source of S9: Not stated
- method of preparation of S9 mix:
- concentration or volume of S9 mix and S9 in the final culture medium: 0.5ml of S.9 mixture was applied to the chemical-top solution. The S.9 mixture contained 0.3 ml of the 9,000 X g supernatant of homognized rat liver
- quality controls of S9 (e.g., enzymatic activity, sterility, metabolic capability): Not stated - Test concentrations with justification for top dose:
- The test material was tested at 200, 400, 600, 800, 1000 ug/plate in tests with and without metabolic activity. No justification on test concentrations were provided in the study report
- Vehicle / solvent:
- - Solvent used: DMSO
- Justification for choice of solvent/vehicle: A justification for the choice of solvent was not provided in the study report.
- Justification for percentage of solvent in the final culture medium: A justification for the percentage solvent in the final culture medium was not provided in the study report.
Controls
- Untreated negative controls:
- yes
- Remarks:
- - S.9 control
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- yes
- Remarks:
- -S.9 control
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- N-ethyl-N-nitro-N-nitrosoguanidine
- other: 2- Aminoanthracene
- Details on test system and experimental conditions:
- NUMBER OF REPLICATIONS:
- Number of cultures per concentration: Duplicate
- Number of independent experiments: Two
METHOD OF TREATMENT/ EXPOSURE:
- Cell density at seeding (if applicable): Not stated
- Test substance added in medium; A solution of the test material was added to the top agar solution where the solution was then mixed and added to the agar plate. For the metabolic activation system the addition of S.9 mixture was added to the mixture prior to applying to the agar plate.
TREATMENT AND HARVEST SCHEDULE:
- Preincubation period, if applicable: N/A
- Exposure duration/duration of treatment:49 hours
- Harvest time after the end of treatment (sampling/recovery times): Not stated
METHODS FOR MEASUREMENTS OF GENOTOXICIY
Not stated - Rationale for test conditions:
- A rationale for the test conditions was not provided in the study report.
- Evaluation criteria:
- The criteria used to determine the mutagenic potential of the test substance was a significant/lack of increase in the spontaneous mutation frequency.
- Statistics:
- No statistics are provided in the study report.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: No mutagenic potential
Any other information on results incl. tables
Table 1: Mutagenic activity of 1-Butene, 4-Bromo-3,3,4,4-Tetrafluoro- in Salmonella Typhimurium strains TA 1535, TA 1537, TA 1538, TA 98 and TA 100 with metabolic activation
|
|
|
Histidine+ Revertants Per Plate ** |
||||
Compound added |
TA 1535 |
TA 1537 |
TA 1538 |
TA 98 |
TA 100 |
||
DMSO |
|
|
13 |
17 |
21 |
37 |
101 |
-S.9* |
(1000 µg/plate) |
12 |
10 |
7 |
18 |
100 |
|
10, 943 |
200 µg/plate |
7 |
11 |
18 |
37 |
98 |
|
|
400 µg/plate |
9 |
17 |
20 |
35 |
98 |
|
|
600 µg/plate |
12 |
20 |
22 |
42 |
118 |
|
|
800 µg/plate |
12 |
14 |
21 |
45 |
98 |
|
|
1000 µg/plate |
13 |
13 |
14 |
29 |
100 |
|
2AA |
5 µg/plate |
- |
- |
- |
- |
933 |
|
|
10 µg/plate |
209 |
- |
782 |
2561 |
- |
|
|
100 µg/plate |
- |
284 |
- |
- |
- |
DMSO = Dimethylsulfoxide (solvent control)
10,943 = 1-butene, 4-bromo-3,3,4,4-tetrafluoro-
2AA = 2-Aminoanthracene (positive control)
* = Test plate without S.9 Activation
** = Average of two plates
Table 2: Mutagenic activity of 1-Butene, 4-Bromo-3,3,4,4-Tetrafluoro- in Salmonella Typhimurium strains TA 1535, TA 1537, TA 1538, TA 98 and TA 100 without metabolic activation
|
|
|
Histidine+ Revertants Per Plate * |
||||
Compound added |
TA 1535 |
TA 1537 |
TA 1538 |
TA 98 |
TA 100 |
||
DMSO |
|
|
4 |
14 |
7 |
22 |
93 |
10, 943 |
200 µg/plate |
9 |
16 |
13 |
20 |
91 |
|
|
400 µg/plate |
6 |
23 |
15 |
29 |
83 |
|
|
600 µg/plate |
9 |
19 |
9 |
22 |
95 |
|
|
800 µg/plate |
9 |
15 |
11 |
26 |
78 |
|
|
1000 µg/plate |
5 |
15 |
9 |
26 |
101 |
|
MNNG |
2 µg/plate |
1966 |
|
- |
- |
1278 |
|
9AAc |
1050 µg/plate |
- |
2022 |
-- |
- |
- |
|
2NF |
10025 µg/plate |
- |
- |
1964 |
3915 |
- |
DMSO = Dimethylsulfoxide (solvent control)
10,943 = 1-butene, 4-bromo-3,3,4,4-tetrafluoro-
MNNG = N-Methyl-N’-nitro-N-nitrosoguanidine (positive control)
9AAc = 9-Aminoacridine
2NF = 2-ANitrofluorene (positive control)
* = Average of two plates
Applicant's summary and conclusion
- Conclusions:
- Under the conditions of this study, the test item is considered not mutagenic in concentrations up to 1000 ug/plate in either the presence or absence of a liver microsomal system on Salmonella typhimurium strains TA 1535, TA 1537, TA 1538, TA 98 and TA 100.
- Executive summary:
The study was performed to assess the mutagenic potential of the test material to Salmonella typhimurium strains TA 1535, TA 1537, TA 1538, TA 98 and TA 100 in the presence and absence of a liver microsomal system (S.9). For treatments in the absence of the liver microsomal system a volume of 0.1mL test substance and 108 bacterial were added to 2ml of the top agar. This solution was applied to the surface of the Davis minimal agar plate.
Treatment in the presence of the liver microsomal system were the same as that in the absence of the liver microsomal system with the exception that 0.5 mL S.9. mixture was added to the chemical topped agar solution. All plates were incubated at 37˚C for 48 hours. Positive and negative controls were used in both test systems and it was not indicated if these were within the acceptable historical control ranges. No applicability criteria were provided for this test method therefore this could not be determined. Under the conditions of the study, the test substance showed no evidence of mutagenic activity and it was concluded to be negative as it did not significantly increase the spontaneous mutation frequency in Salmonella typhimurium.
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