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Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
17 January 2001 until 26 June 2001
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
activated sludge
Details on inoculum:
Preparation of the inoculum:
The inoculum was washed, brought to a concentration of 5 g/I dry substance and aerated during the night. 50 ml were added to a total volume of 250 ml to obtain a concentration of 1 g/I dry substance in the test.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
30 min
Test temperature:
20 +-2°C
pH:
7.6
Dissolved oxygen:
Oxygen concentration during aeration: > 2.5 mg/l
Oxygen concentration immediately before measurement: > 6.5 mg/l
Duration of the measurement of oxygen consumption: 8 - 10 min
Nominal and measured concentrations:
Nominal concentration: 1000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer-vessel
- Fill volume: 250 ml
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol
Key result
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Results with reference substance (positive control):
EC20 = 2.5 mg/L
EC50 = 17 mg/L
EC80 = 75 mg/L
Validity criteria fulfilled:
yes
Conclusions:
Based on the nominal concentrations the EC50 is greater than 1000 mg/L.
Executive summary:

The study was performed to determine the toxic effect on activated sludge in this 30 minutes respiration inhibition test.


Based on the nominal concentrations the EC50 is greater than 1000 mg/L. No significant effect was observed in this study.

Description of key information

In a 30 min Respiration inhibition Test (BASF, 00/0802/08/1 the toxic effect on activated sludge was observed. No statistically significant effect was observed. The EC50 is > 1000 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:
1 000 mg/L

Additional information