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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Link to relevant study record(s)

Reference
Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
16 January 1997 - 30 January 1997
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
not specified
Vehicle:
not specified
Details on preparation and application of test substrate:
Artificial soil test substrate composition
- 10% sphagnum peat
- 20% kaolin clay
- 70% industrial sand (fine sand was dominant with more than 50% of the particles between 50 and 200 microns).
pH is adjusted to 6.0 rt 0.5 by addition of calcium carbonate.

Method of preparation
The dry constituents were blended in the correct proportions and mixed thoroughly, in the large-scale laboratory mixer Cutter VB. Moisture content was then determined by drying a small sample at 105OC and re-weighing. Deionised water was added to give an overall moisture content of about 30% of the dry weight, and the medium was thoroughly mixed.

TEST ARTICLE FORMULATES
Suitable volumes of a 10% test article solution in acetone were mixed with small amounts of dry soil, after evaporation of the solvent, subsequently added to the rest of the soil. The soil was mixed with the large laboratory mixer Cutter VB for about 1 minute and the moisture content was raised to the required level by addition of distilled water.
Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
Justificcation for the selection of the test system: the Eisenia foetida was chosen as an earthworm species, since it is widely accepted by Health Authorities as an experimental model with documented susceptibility to a wide range of toxic substances.

Number: 40 animals/concentration subdivided into 4 replicates (control included)

Body weight and age: the earthworms used were adult with a well-developed clitellium, more than 6 weeks old, with an average weight of 320 mg.

Supplier: laboratory breeding. Eisenia foetida are bred at an average of 20 OC in plastic boxes of about 32x27x25 cm under continuous illumination, the medium consists of 50:50 mixture of horse manure and peat, at
pH 7.0.

Animal pre-treatment: earthworms were pre-conditioned for 24 hours in the untreated artificial soil under the same conditions of the test and then quickly washed.
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
yes
Total exposure duration:
14 d
Test temperature:
20°C +/-2"C.
pH:
6.4 - 6.8
Moisture:
19.1-30%
Details on test conditions:
Experimental design: Experimental design: on the basis of preliminary results, the test article was tested at the nominal concentration of 1000 mglkg (dried soil) (Limit test). One group of animals was treated only with water (control group).
Contain ers: the containers consisted of 1000 ml glass beakers.

Preparation of the containers: for each treatment group, 750 g wet weight of the test medium were placed into each glass container and then 10 pre-treated earthworms were placed on the medium surface. The containers were covered with perforated plastic film to prevent the test medium from drying.

Treatment frequency: single.

Environmental conditions: the test was performed in continuous light at 20°C +/-2"C..

Observation period: 14 days.

Observation of visible abnormalities and mortality the animals were inspected after 7 and 14 days.
Worms are classified as dead when they do not respond to a gentle mechanical stimulus to the front end. Any behavioral or pathologial signs noted were reported.

Parameter checking: moisture content of artificial soil at start and end of the test, pH value at start of the test.
Nominal and measured concentrations:
Nominal concentration 1000 mg/kg
Key result
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
OBSERVATION OF VISIBLE ABNORMALITIES
No animals of the control and treated groups showed visible signs of abnormalities.

MORTALITY
No animals of the control and the treated groups died.

PARAMETER CHECKING
The values of moisture content and pH of the artificial soil remained within the acceptable limits.

LC50 AND ITS STATISTICAL LIMITS
LC50 at 7 and 14 days were not calculable.

Conc. 0 mg/kg (control group)

Day

Moisture content (%)

pH

0

30

6.4

14

22.5

-

 

Conc. 0 mg/kg (solvent control group)

Day

Moisture content (%)

pH

0

30

6.4

14

19.9

-

 

Conc. 1000 mg/kg

Day

Moisture content (%)

pH

0

30

6.8

14

19.1

-

 

Validity criteria fulfilled:
yes
Conclusions:
Experimental data from an acute "artificial soil" toxicity study in which earthworms were treated with the test article ANOX BF at the nominal concentration of 1000 mg/kg (dried soil) are given in this report.
The animals were treated and observed for 14 days. No animals in the control and the treated group died or showed signs of visible abnormalities.

The LC50 values at 7 and 14 days were not calculable.
Executive summary:

Experimental data from an acute "artificial soil" toxicity study in which earthworms were treated with the test article ANOX BF at the nominal concentration of 1000 mg/kg (dried soil) are given in this report.

The animals were treated and observed for 14 days. No animals in the control and the treated group died or showed signs of visible abnormalities.

The LC50 values at 7 and 14 days were not calculable.

Description of key information

Experimental data from an acute "artificial soil" toxicity study in which earthworms were treated with the test article ANOX BF at the nominal concentration of 1000 mg/kg (dried soil) are given in this report.
The animals were treated and observed for 14 days. No animals in the control and the treated group died or showed signs of visible abnormalities.

The LC50 values at 7 and 14 days were not calculable.

Key value for chemical safety assessment

Additional information