Reaction mass of 2-((RS)-1-((SR)-3,3-dimethylcyclohexyl)ethoxy)-2-methylpropan-1-ol and 2-((RS)-1-((RS)-3,3-dimethylcyclohexyl)ethoxy)-2-methylpropan-1-ol and 2-methyl-2-(((1RS,2RS)-2,6,6-trimethylcycloheptyl)oxy)propan-1-ol

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

June 11, 2009 to January 07, 2010

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

1992

Deviations:

yes

Remarks:

oxygen consumptions were registered in a rythm of 1.1 days instead of daily.

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Deviations:

yes

Remarks:

oxygen consumptions were registered in a rythm of 1.1 days instead of daily.

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 835.3110 (Ready Biodegradability)

Deviations:

yes

Remarks:

oxygen consumptions were registered in a rythm of 1.1 days instead of daily.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-methyl-2-(((1RS,2RS)-2,6,6-trimethylcycloheptyl)oxy)propan-1-ol

Molecular formula:

C14H28O2

IUPAC Name:

2-methyl-2-(((1RS,2RS)-2,6,6-trimethylcycloheptyl)oxy)propan-1-ol

Constituent 2

Reference substance name:

2-((RS)-1-((SR)-3,3-dimethylcyclohexyl)ethoxy)-2-methylpropan-1-ol

Molecular formula:

C14H28O2

IUPAC Name:

2-((RS)-1-((SR)-3,3-dimethylcyclohexyl)ethoxy)-2-methylpropan-1-ol

Constituent 3

Reference substance name:

2-((RS)-1-((RS)-3,3-dimethylcyclohexyl)ethoxy)-2-methylpropan-1-ol

Molecular formula:

C14H28O2

IUPAC Name:

2-((RS)-1-((RS)-3,3-dimethylcyclohexyl)ethoxy)-2-methylpropan-1-ol

Test material form:

liquid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage (City of Geneva, Peney-Dessous) was used.
The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day.

Duration of test (contact time):

66 d

Initial test substance concentration

Initial conc.:

30 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Prepared by mixing 50 ml of solution A and 2000 ml deionised water, adding 5 ml of each of the solutions B, C and D and making up to 5 liters with deionised water. The pH is measured and if necessary adjusted to 7.4 +/- 0.2 with phosphoric acid or potassium hydroxide.

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

Test performance:

Everyday the oxygen consumption of each flask is recorded and correct temperature and stirring are checked.
At the end of the test period, the pH of each flask is measured again.
The respirometer used during this study is an Oxitop Control System, made by Wissenschaftlich-Technische Werkstätten, Weilheim, Germany.

% Degradation

% Degradation 1

Key result

Parameter:

% degradation (O2 consumption)

Value:

2

Sampling time:

28 d

% Degradation 2

Parameter:

% degradation (O2 consumption)

Value:

6

Sampling time:

66 d

BOD5 / COD results

Results with reference substance:

Degradation of sodium benzoate exceeds 40 % after 7 days and 65 % after 14 days: the activity of the inoculum is thus verified (validity criterion).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The ready biodegradability of Serenol was determined by the Manometric Respirometry Test.
Serenol udergoes 2% of biodegradation after 28 days (6% after 66 days) in the test conditions.

The curves obtained with the reference substance alone and with Serenol + reference substance show no toxic effect of Serenol to the micro-organisms at the test concentration (30 mg/L).

Executive summary:

The ready biodegradability of Serenol was determined by the Manometric Respirometry Test.

Serenol udergoes 2% of biodegradation after 28 days (6% after 66 days) in the test conditions.

The curves obtained with the reference substance alone and with Serenol + reference substance show no toxic effect of Serenol to the micro-organisms at the test concentration (30 mg/L).

Thus, Serenol should be regarded as not readily biodegradable according to this test.