4-[(2E)-3-(3,5-dichloro-4-fluorophenyl)-4,4,4-trifluorobut-2-enoyl]-N-[(4R)-2-ethyl-3-oxo-1,2-oxazolidin-4-yl]-2-methylbenzamide

Skin sensitisation

Administrative data

Endpoint:

skin sensitisation: in chemico

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2020

Report date:

2020

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 442C (In Chemico Skin Sensitisation: Direct Peptide Reactivity Assay (DPRA))

Version / remarks:

2019

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of study:

direct peptide reactivity assay (DPRA)

Justification for non-LLNA method:

In vitro/chemico assay to be conducted instead of in vivo test.

Test material

Test material information

Constituent 1

Reference substance name:

4-[3-(3,5-dichloro-4-fluorophenyl)-4,4,4-trifluorobut-2-enoyl]-N-(2-ethyl-3-oxo-1,2-oxazolidin-4-yl)-2-methylbenzamide

Molecular formula:

C23H18Cl2F4N2O4

IUPAC Name:

4-[3-(3,5-dichloro-4-fluorophenyl)-4,4,4-trifluorobut-2-enoyl]-N-(2-ethyl-3-oxo-1,2-oxazolidin-4-yl)-2-methylbenzamide

Test material form:

solid

Details on test material:

Off-white

In chemico test system

Details on the study design:

The Direct Peptide Reactivity Assay (DPRA) is an in chemico method which quantifies the remaining concentration of cysteine- or lysine-containing peptide following 24 hours incubation with the test item at 25 °C. The synthetic peptides contain phenylalanine to aid in the detection. The relative peptide concentration is measured by high-performance liquid chromatography (HPLC) with gradient elution and spectrophotometric detection at 220 nm and 258 nm. Cysteine and lysine peptide Percent Depletion Values are calculated and used in a prediction model which allows assigning the test item to one of four reactivity classes used to support the discrimination between sensitizers and non-sensitizers.

Results and discussion

In vitro / in chemico

Results

Key result

Remarks on result:

not determinable because of methodological limitations

Remarks:

The substance was not soluble in any of the solvents tested and the DPRA study could not be performed.

Applicant's summary and conclusion

Interpretation of results:

study cannot be used for classification

Conclusions:

The DPRA study could not be performed as the substance was not soluble in any suitable solvent.

Executive summary:

The objective of the study was to determine the skin sensitisation potential of the substance under GLP to OECD TG 442c, and test the reactivity of the test substance towards synthetic peptides containing cysteine (SPCC) or lysine (SPCL). At a concentration of 100 mM, the test substance was not soluble in acetonitrile, Milli-Q-water or a 1:1 v/v mixture of the two, isopropanol, a mixture of acetone and acetonitrile (1:1, v/v), dimethylsulphoxide and acetonitrile (1:9 v/v), methanol or ethanol. Consequently, the Direct Peptide Reactivity Assay (DPRA) could not be performed with the substance and the study was stopped.