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EC number: 201-126-0 | CAS number: 78-59-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Repeated dose toxicity: oral
Administrative data
- Endpoint:
- sub-chronic toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- 90-Day subchronic toxicity of isophorone in the rat.
- Author:
- Rohm & Haas Co. / Affiliated Medical Research, Inc., Princeton, N.J.
- Year:
- 1 972
- Bibliographic source:
- Contract No. 120-1008-61, NTIS/OTS 0205975, Doc No. 878212178
- Reference Type:
- review article or handbook
- Title:
- Isophorone: Ambient water quality criteria.
- Author:
- U.S. EPA (Environmental Protection Agency)
- Year:
- 1 978
- Bibliographic source:
- Report No. PB-296 798, U.S. Department of Commerce / National Technical Information Service
- Reference Type:
- other: OECD SIDS
- Title:
- 3,5,5-Trimethylcyclohex-2-enone (Isophorone), CAS No. 78-59-1
- Author:
- OECD SIDS
- Year:
- 2 003
- Bibliographic source:
- SIDS Initial Assessment Report for SIAM 16
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Male and female CFE rats were orally treated with three different doses of the test substance administered via their diet daily for a period of 90 days.
- GLP compliance:
- no
- Limit test:
- no
Test material
- Reference substance name:
- 3,5,5-trimethylcyclohex-2-enone
- EC Number:
- 201-126-0
- EC Name:
- 3,5,5-trimethylcyclohex-2-enone
- Cas Number:
- 78-59-1
- Molecular formula:
- C9H14O
- IUPAC Name:
- 3,5,5-trimethylcyclohex-2-enone
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: CFE
- Sex:
- male/female
Administration / exposure
- Route of administration:
- oral: feed
- Vehicle:
- unchanged (no vehicle)
- Analytical verification of doses or concentrations:
- not specified
- Duration of treatment / exposure:
- 90 days
- Frequency of treatment:
- daily
Doses / concentrationsopen allclose all
- Dose / conc.:
- 750 ppm
- Remarks:
- corresponds to 57 mg/kg bw/d in males and 78.9 mg/kg bw/d in females
- Dose / conc.:
- 1 500 ppm
- Remarks:
- corresponds to 102.5 mg/kg bw/d in males and 163.8 mg/kg bw/d in females
- Dose / conc.:
- 3 000 ppm
- Remarks:
- corresponds to 233.8 mg/kg bw/d in males and 311.8 mg/kg bw/d in females
- No. of animals per sex per dose:
- 20
After 4 weeks, 5 animals per sex and dose group were killed for blood analysis. - Control animals:
- yes, concurrent no treatment
- Details on study design:
- Post-exposure period: none
Examinations
- Observations and examinations performed and frequency:
- Clinical signs: daily
Mortality: daily
Body weight: weekly
Food consumption: weekly
Water consumption: weekly
Ophthalmoscopic examination: no
Hematology: after 4 weeks and at the end of the study: determination of hemoglobin, hematocrit, erythrocyte counts, leukocyte counts, and differential leukocyte determinations
Biochemistry: after 4 weeks and at the end of the study: blood glucose, blood urea nitrogen, serum glutamic oxaloacetic transaminase, serum alkaline phosphatase, total serum protein, total serum bilirubin, serum albumin, lactic acid dehydrogenase, cholesterol, calcium, phosphate, and uric acid
Urinalysis: after 4 weeks and at the end of the study: pH, glucose, ketones, albumin, occult blood, microscopic examination of sediment - Sacrifice and pathology:
- ORGANS EXAMINED AT NECROPSY (MACROSCOPIC AND MICROSCOPIC)
Organ weights: Organ-body weight ratios: heart, liver, kidney, adrenals, thyroid, brain, testes
Macroscopic: After 4 weeks and at study termination: lungs, heart, intestines, kidneys, spleen, liver, urinary bladder
Weights: Heart, liver, kidney, adrenals, thyroid, brain, testes (males) for 10 males and 10 females of each dose level
Microscopic: At study termination: 5 males and 5 females each from high dose and control groups: brain, pituitary, eye, thyroid, lung, heart, liver, kidney, adrenals, urinary bladder, mediastinal lymph node, pancreas, spleen, colon, bone marrow, skeletal muscle, testes and prostate (male), ovary and uterus (female). 5 males and 5 females each from medium and low dose groups: liver, kidney. - Statistics:
- All data were evaluated statistically.
Results and discussion
Results of examinations
- Clinical signs:
- no effects observed
- Mortality:
- mortality observed, non-treatment-related
- Description (incidence):
- 1 male (control group), 1 female (3000 ppm): deaths were due to intercurrent infection
- Description (incidence and severity):
- - 3000 ppm, males: significantly reduced body weight gain (P < 0.01); further observed changes returned to normal in the subsequent weeks
- Food consumption and compound intake (if feeding study):
- no effects observed
- Food efficiency:
- not examined
- Water consumption and compound intake (if drinking water study):
- no effects observed
- Ophthalmological findings:
- not examined
- Haematological findings:
- no effects observed
- Clinical biochemistry findings:
- no effects observed
- Urinalysis findings:
- no effects observed
- Behaviour (functional findings):
- not examined
- Immunological findings:
- not examined
- Organ weight findings including organ / body weight ratios:
- effects observed, non-treatment-related
- Description (incidence and severity):
- - kidney, testes, males: slightly increased mean organ to body weight ratio (considered not compound-related by the authors; not statistically significant)
- Gross pathological findings:
- no effects observed
- Neuropathological findings:
- not examined
- Histopathological findings: non-neoplastic:
- no effects observed
- Histopathological findings: neoplastic:
- no effects observed
Effect levels
open allclose all
- Dose descriptor:
- NOAEL
- Remarks:
- corresponding to 311.8 mg/kg bw/d
- Effect level:
- 3 000 ppm
- Based on:
- test mat.
- Sex:
- female
- Basis for effect level:
- other: no adverse effects up to and including the highest tested dose
- Dose descriptor:
- NOAEL
- Remarks:
- corresponding to 102.5 mg/kg bw/d
- Effect level:
- 1 500 ppm
- Based on:
- test mat.
- Sex:
- male
- Basis for effect level:
- other: reduced body weight gain
Target system / organ toxicity
- Critical effects observed:
- no
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.