Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 855-895-8 | CAS number: 212908-67-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2007-07-13 to 2007-08-16
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- adopted 1992-07-17
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-hydroxy-N-[(2S,3S,4R)-1,3,4-trihydroxyoctadecan-2-yl]benzamide
- EC Number:
- 855-895-8
- Cas Number:
- 212908-67-3
- Molecular formula:
- C27H47NO5
- IUPAC Name:
- 2-hydroxy-N-[(2S,3S,4R)-1,3,4-trihydroxyoctadecan-2-yl]benzamide
- Test material form:
- solid: bulk
- Details on test material:
- Purity: > 99.8%
Lot No.: C928/06/4/1
Constituent 1
- Specific details on test material used for the study:
- As the test substance is not water soluble or homogeneously suspendable in water it was directly weighed into the 3 test vessels, so that the final concentration was 100 mg/L in inoculated mineral medium.
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- A fresh sample of activated sludge from the aeration basin of a municipal sewage treatment plant
(Moers, Germany) was collected and aerated until usage. The inoculum was not fed during the aeration period.
The duration of the aeration period was 1 day.
After determination of the suspended solids (dry wweight) a sample containing 1875 mg suspended solids was
filtered by means of a water jet was washed twice with mineral medium. Afterwards, it was suspended
in 250 mL mineral medium and stirred until it was homogeneous. 4 mL of this suspension were used to
inoculate 1 liter of mineral medium. This amount of inoculum corresponded to 30 mg/L suspended solids. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral salts medium according to OECD 301 F
- Test temperature: 22 °C
- pH: 6.0 - 8.5
- Continuous darkness: yes
TEST SYSTEM, CONTROL AND BLANK SYSTEM
- Culturing apparatus: vessels
- Number of culture flasks/concentration:
three for the blank control
two for the abiotic control (test substance + HgCl2)
two for the reference substance (sodium benzoate)
three for the test substance
two for the toxicity control (test substance + sodium benzoate)
- Method used to create aerobic conditions: aeration 1 days
- Inhibition of nitrification: addition of N-allylthiourea (N-ATH)
- Laboratory equipment:
Digital Respirometer System, Sensomat system, Liebherr, Aqualytic, Langen, Germany
pH-Meter, WTW, Weilheim Germany
heater device: CSB Thermostat, Ströhlein Instruments, Germany
titration device: 686 Titroprocessor, Metrohm, Herisau, Schwitzerland
normal laboratory equipment
For further details on test system, please see section “any other information on materials and methods incl. tables”.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 100 mg/L
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 80
- Sampling time:
- 28 d
- Remarks on result:
- other: biodegradation rate as % BOD
- Details on results:
- For a detailed description of results, please see section "any other information on results incl. tables".
BOD5 / COD results
- Results with reference substance:
- The reference substance was biodegraded to 89% within 14 days.
Any other information on results incl. tables
The following biodegradation rates were reached:
Test vessel (No.) |
Test compound |
Degradation rate in [%] 14d |
Degradation rate in [%] 10d window day 4 – day 14 |
Degradation rate in [%] 28d |
Mean value±standard deviation [%] |
4 |
100 mg/L test substance (abiotic control) |
n.r. |
n.r. |
-1 |
|
5 |
100 mg/L test substance (abiotic control) |
n.r. |
n.r. |
-1 |
-1 / 0 |
6 |
100 mg/L sodium benzoate |
89 |
n.r. |
99 |
|
7 |
100 mg/L sodium benzoate |
89 |
n.r. |
94 |
96 /±4 |
8 |
100 mg/L test substance |
n.r. |
52 |
72 |
|
9 |
100 mg/L test substance |
n.r. |
61 |
84 |
|
10 |
100 mg/L test substance |
n.r. |
63 |
84 |
80 /±7 |
11 |
100 mg/L test substance + 100 mg/L sodium benzoate |
60 |
n.r. |
77 |
|
12 |
100 mg/L test substance + 100 mg/L sodium benzoate |
57 |
n.r. |
71 |
74 /±4 |
n.r. = not relevant
Biodegradation rates of the test substance N-Salicyloyl-Phytosphingosine were calculated on the basis of its Chemical Oxygen Demand, which was 1663 mg Oxygen per gram (Study No. 17041, Work and Final Report No. 405/2007).
Biodegradation rates of the reference substance sodium benzoate were calculated on the basis of its Chemical Oxygen Demand, which was 1638 mg Oxygen per gram (Study No. 16350, Work and Final Report No. 71/2005).
Mean values of biodegradation for the test substance as determined in a manometric respiratory test:
|
Biodegradation in % of COD |
|
|
10 day window / Biodegradation after 14 days |
Biodegradation after 28 days |
Test substance1 |
59 |
80 |
Reference Substance2 |
89 |
96 |
Toxicity Control2 |
58 |
74 |
Abiotic control |
Not relevant |
-1 |
1 = 10 day window applies, 2 = biodegradation after 14 days applies
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- N-Salicyloyl-Phytospingosine is classified as readily biodegradable within the 28 day period of the study, but failing the 10-day window.
- Executive summary:
The ready biodegradability of N-Salicyloyl-Phytospingosine was determined with non-adapted activated
sludge in the Manometric Respirometry Test for a period of 28 days. The study was conducted
according to OECD guideline 301 F. The concentration of N-Salicyloyl-Phytospingosine selected as
appropriate was 100 mg/L. The oxygen was depleted by the respiration of bacteria
and the degradation was followed by measuring the oxygen concentration. The biodegradation
rate is therefore expressed as the percentage BOD (biological oxygen demand) and was
calculated for each study day.
Sodium benzoate was used as reference substance. The pass level > 60 % was reached.
The functional capability of the inoculum was sufficient, as the reference substance sodium
benzoate was biodegraded to an average of 89% within 14 days.
The test substance is not assumed to be inhibitory, because 58% degradation accured within
14 days in the toxicity control preparations containing 100 mg test substance and 100 mg sodium benzoate per liter.
The average degradation value of the test substance was 80% after 28 days. The test substance
was biodegraded to 59% in a 10-d window (day 4-14) within the 28-d period at the recommended concentration of 100 mg/L.
N-Salicyloyl-Phytospingosine is classified as readily biodegradable within the 28 day period of the study,
but failing the 10-day window.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.