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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Dissociation constant
Administrative data
- Endpoint:
- dissociation constant
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Study initiation date: July 8, 2015: Study completion date: December 1, 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 112 (Dissociation Constants in Water)
- GLP compliance:
- not specified
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Type:
- impurity
- Test material form:
- solid: particulate/powder
Results and discussion
- Dissociating properties:
- yes
Dissociation constant
- Temp.:
- 40 °C
- Remarks on result:
- other: As determined by HPLC, it was concluded that the test item has undissociated form less than pH 1.2
Any other information on results incl. tables
Confirmation of solution stability of test item results and discussion
The change of the spectrum was not confirmed in acid solution. The change of the spectrum was confirmed in the buffer solution (pH 4.2) and alkaline solution. Therefore, the spectrometric method was not able to apply because the test item was changed in the pH range where dissociation of the test item was expected.
Evaluation of dissociated/undissociated form by HPLC results and discussion
The retention times of the test item are shown in the table below. The peaks of the test item were not detected in the eluent (more than pH 5.0) and the peaks of converted products were detected. Therefore, it was difficult to evaluate the dissociated/undissociated form of the test item at more than pH 5.0.
In view of the reverse phase HPLC analysis for dissociative substances, it is well known that the retention time become the maximum and constant at undissociated form. In this study, the retention times of the test item were approximately constant less than pH 1.2. Therefore, it was considered that the test item has undissociated form in less than pH 1.2.
pH of buffer solution |
Retention time (min) |
Remarks |
|||
Measurement value |
Average |
||||
1.0 |
Main component |
10.46 |
10.50 |
10.48 |
- |
Sub component |
29.96 |
30.01 |
29.99 |
||
1.2 |
Main component |
10.30 |
10.32 |
10.31 |
- |
Sub component |
29.79 |
29.81 |
29.80 |
||
2.0 |
Main component |
9.50 |
9.53 |
9.52 |
- |
Sub component |
28.83 |
28.83 |
28.83 |
||
4.0 |
Main component |
6.33 |
6.33 |
6.33 |
- |
Sub component |
19.00 |
19.00 |
19.00 |
||
5.0 |
Main component |
- |
- |
- |
Converted products were detected |
Sub component |
- |
- |
- |
||
7.0 |
Main component |
- |
- |
- |
Converted products were detected |
Sub component |
- |
- |
- |
||
10.0 |
Main component |
- |
- |
- |
Converted products were detected |
Sub component |
- |
- |
- |
||
12.0 |
Main component |
- |
- |
- |
Converted products were detected |
Sub component |
- |
- |
- |
Applicant's summary and conclusion
- Conclusions:
- The dissociation constant was not able to measured by the spectrometric method because the test item was changed in the pH range where dissociation of the test item was expected. Therefore, the dissociated/undissociated form of the test item was evaluated using HPLC and it is concluded that the test item has undissociated form less than pH 1.2.
- Executive summary:
Test item: CIM-43
Objective: This study was performed to evaluate the dissociated/undissociated form of CIM-43
Test method: OECD guidelines for testing of chemical No.112, May 12, 1981, “Dissociation constants in water: Spectrometric Method”
The spectrometric method was not able to apply because the test item was converted in the test solution. Therefore, the dissociated/undissociated form of the test item was evaluated by high-performance liquid chromatography (HPLC).
Results: The dissociation constant was not able to measure by the spectrometric method because the test item was changed in the pH range where dissociation of the test item was expected. Therefore, the dissociated/undissociated form of the test item was evaluated using HPLC and it is concluded that the test item has undissociated form less than pH 1.2.
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