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EC number: 941-682-8 | CAS number: 1476113-93-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- 1997
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- N-{[5-chloro-2-(propan-2-yl)phenyl]methyl}cyclopropanamine
- EC Number:
- 941-682-8
- Cas Number:
- 1476113-93-5
- Molecular formula:
- C13 H18 Cl N
- IUPAC Name:
- N-{[5-chloro-2-(propan-2-yl)phenyl]methyl}cyclopropanamine
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- cofactor-supplemented post-mitochondrial fraction (S9 mix), prepared from the livers of male rats, treated with phenobarbital and β-naphthoflavone
- Test concentrations with justification for top dose:
- Initial Mutation Test (Exp.1) and Confirmatory Mutation Test (Exp.2):
- 0.5, 1.581, 5, 15.81, 50, 158.1, 500, 1581 μg/plate (with and without metabolic activation, all Salmonella strains)
- 5, 15.81, 50, 158.1, 500, 1581 and 5000 μg/plate (with and without metabolic activation, E. coli WP2 uvrA)
Complementary Confirmatory Mutation Test (Exp.3):
- 0.05, 0.1581, 0.5, 1.581, 5, 15.81, 50, 158.1 μg/plate (without metabolic activation, all Salmonella strains)
- 0.1581, 0.5, 1.581, 5, 15.81, 50, 158.1 and 500 μg/plate (with and without metabolic activation, E. coli WP2 uvrA) - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- sodium azide
- methylmethanesulfonate
- other: 4-nitro-1,2-phenylenediamine (-S9-mix: TA98), 2-aminoanthracene (+ S9-mix: all tester strains)
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- other: S. typhimurium TA98, TA100, TA1535 and TA1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- In Exp.1 cytotoxicity was observed in all 4 Salmonella strains starting at 500 μg/plate with and without S9-mix. In addition, in TA1537 the number of revertants decreased to less than half at 50 µg/plate without and at 158.1 μg/plate with S9-mix.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- In Exp. 1 cytotoxicity was observed starting at 1581 μg/plate with S9-mix and starting at 500 μg/plate without S9-mix. In Exp.2 excessive cytotoxicity was observed starting at 158.1 μg/plate with and without S9-mix.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Remarks on result:
- other: In the Exp.2 excessive cytotoxicity was observed in TA98, TA100 and TA1537 starting at 50 μg/plate without S9-mix and starting at 158.1 μg/plate with S9-mix.
- Remarks:
- In TA1535 excessive cytotoxicity was observed in Exp.2 at 158.1 μg/plate without S9-mix and starting at 158.1 μg/plate with S9-mix. In Exp. 3 the number of revertants decreased to less than half in TA98 and TA100 at 158.1 μg/plate and in TA98 additionally at 50 μg/plate without S9-mix.
Applicant's summary and conclusion
- Conclusions:
- The potential of the test substance to induce gene mutations was examined in 4 Salmonella typhimurium strains TA98, TA100, TA1535 and TA1537 and in the Escherichia coli WP2 uvrA strain in two independent experiments, which were carried out without and with metabolic activation. Due to excessive cytotoxicity in one of the other experiments a third, additional experiment was carried out with and without metabolic activation in the E.coli strain, but only without metabolic activation in the four Salmonella strains. The first experiment was carried out as a plate incorporation test and the second and third experiment as a preincubation test. The test material was considered to be non-mutagenic either in the presence or absence of metabolic activation in the plate incorporation as well as in the preincubation test.
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