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EC number: 617-638-6 | CAS number: 84868-02-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1992-04-24 to 1992-04-26
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 1984
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Controls, 0.1; 0.18; 0.32; 0.58 and 1.0 test item mg/L
- Sampling method: At the beginning of the experiments, for each concentration an additional solution (100 mL) was prepared under the same conditions as the test solution for the analytical verification. The bottles were sealed and stored at <4 °C. 20 mL of the samples were sent to the client for analytical verification. At the end of the experiment, the daphnia were removed and 100 mL of each sample were treated analogous to the samples at the beginning of the experiment. Of these samples also 20 mL each were sent to the client for analytical verification. - Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A series of stock solutions of the test substance were prepared in acetone. By withdrawing 100 μL/L each, the stated nominal concentrations of the test substance were prepared in the test solutions.
- Controls: Yes, vehicle control (100 µL/L acetone) and control containing ony diluton water (without test substance).
- Chemical name of vehicle: acetone
- Concentration of vehicle in test medium: 100 µL/L - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain/clone: STRAUS
- Age at study initiation: < 24 hours
- Feeding during test : No - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 70 mg CaCO3/L
- Test temperature:
- 21 °C
- pH:
- 7.5 (at the start and end of the test)
- Dissolved oxygen:
- 96% (at the start)
89% (at the end) - Nominal and measured concentrations:
- Nominal: Controls, 0.1; 0.18; 0.32; 0.58 and 1.0 mg/L
Measured concentrations:- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beaker
- Material, size, headspace, fill volume: glass, 100 mL, none, 100 mL
- Volume of solution: 100 mL
- Aeration: No
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Standard dilution water according to OECD 202
EFFECT PARAMETERS MEASURED: Immobilisation of the Daphnia was recorded after 24h and 48h. Daphnia was considered immobilized if the animals showed no movement within 15 seconds after a gentle touch. If other effects were observed, they were also noted.
VEHICLE CONTROL PERFORMED: yes
- Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.19 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: No
- Other adverse effects control: No
- Immobilisation of control: No
- Analytical results: The effect concentrations given are based on the nominal concentrations of the test substance. The solutions were analytically checked. No test substance was found in any of the test solutions. All measurements were below the detection limit of 0.1 mg/L. This is related to the very low water solubility of the test substance. However, since the observed effects were nevertheless dependent on the concentration, it can be assumed that the test substance has an effect on the immobilization of the daphnia, even in an unresolved form.Table 1: Cumulative immobilization at 24 h and 48 h, Cumulative percentage immobilization in ( )
Concentration
[mg/L]Immobilized daphnids
24 h
48 h
0
0*
(0)
0
(0)
100 µL acetone/L
0*
(0)
0
(0)
0.1
0*
(0)
0
(0)
0.18
0*
(0)
6
(30)
0.32
10*
(50)
20*
(100)
0.58
20*
(100)
20*
(100)
1.0
20*
(100)
20*
(100)
* 20 daphnia were used at the beginning of the experiment.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute immobilization study with Daphnia magna was performed according to OECD 202 (1992). Under the given conditions the 48h-EC50 value was 0.19 mg/L and the NOEC was 0.1 mg/L.
- Executive summary:
The effects of the test item to Daphnia magna (STRAUS) were determined in an acute immobilisation test according to OECD 202 (1992). The study was conducted under static conditions over a period of 48 hours with nominal test item concentrations of 0.1, 0.18, 0.32, 0.58 and 1.0 mg/L. Acetone was used as a solvent and a vehicle control containing acetone (100 µL/L) and a control (containing only dilution water) was included in the test. Twenty daphnids were exposed to each concentration level and the controls.
The concentrations of the test item were analytically verified via gas chromatography at the start (0 and 24 hours) and at the end of the exposure intervals (24 and 48 hours) in all concentration levels and in the control. No test substance was found in any of the test solutions. All measurements were below the detection limit of 0.1 mg/L. This is related to the very low water solubility of the test substance. However, since the observed effects were nevertheless dependent on the concentration, it can be assumed that the test substance has an effect on the immobilization of the daphnia, even in an unresolved form. Therefore, the effect levels were based on the nominal concentrations of the test item.
Under the given conditions the 48h-EC50 value was 0.19 mg/L and the NOEC was 0.1 mg/L.
Reference
Description of key information
The acute immobilization study with Daphnia magna was performed according to OECD 202 (1992). Under the given conditions the 48h-EC50 value was 0.19 mg/L and the NOEC was 0.1 mg/L (reference 6.1.3-1).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.19 mg/L
Additional information
The effects of the test item to Daphnia magna (STRAUS) were determined in an acute immobilisation test according to OECD 202 (1992). The study was conducted under static conditions over a period of 48 hours with nominal test item concentrations of 0.1, 0.18, 0.32, 0.58 and 1.0 mg/L. Acetone was used as a solvent and a vehicle control containing acetone (100 µL/L) and a control (containing only dilution water) was included in the test. Twenty daphnids were exposed to each concentration level and the controls.
The concentrations of the test item were analytically verified via gas chromatography at the start (0 and 24 hours) and at the end of the exposure intervals (24 and 48 hours) in all concentration levels and in the control. No test substance was found in any of the test solutions. All measurements were below the detection limit of 0.1 mg/L. This is related to the very low water solubility of the test substance. However, since the observed effects were nevertheless dependent on the concentration, it can be assumed that the test substance has an effect on the immobilization of the daphnia, even in an unresolved form. Therefore, the effect levels were based on the nominal concentrations of the test item.
Under the given conditions the 48h-EC50 value was 0.19 mg/L and the NOEC was 0.1 mg/L (reference 6.1.3-1).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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