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EC number: 846-827-8 | CAS number: 1521274-68-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2014
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 492 (Reconstructed Human Cornea-like Epithelium (RhCE) Test Method for Identifying Chemicals Not Requiring Classification and Labelling for Eye Irritation or Serious Eye Damage)
- Version / remarks:
- pre-guideline study
- Deviations:
- yes
- Principles of method if other than guideline:
- Study was conducted pre-guideline but according to a similar protocol. 50 µL bulk volume equivalent to 15 mg of the test substance were incubated for 6 hours followed by a 18-hours post-incubation period
- GLP compliance:
- not specified
Test material
- Reference substance name:
- N-ethylpyridazin-4-amine
- EC Number:
- 846-827-8
- Cas Number:
- 1521274-68-9
- Molecular formula:
- C6H9N3
- IUPAC Name:
- N-ethylpyridazin-4-amine
- Details on test material:
- - State of aggregation: solid/brown
Constituent 1
Test system
- Details on study design:
- - Details of the test procedure used:
Two tissue sample were incubated with the test substance for 6 hours, followed by a 18 hour post-incubation period. The extend of tissue destruction was determined by measuring mitochondrial dehydrogenase activity via MTT test.
- RhCE tissue construct used:
not specified
- Doses of test chemical and control substances used:
test chemical:
50 µL bulk volume (i.e. 15 mg) of undiluted test substance was applied;
negative control:
de-ionized water, sterile
positive control:
methyl acetate (98+%, CAS 79-20-9)
MTT reduction control:
test substance or de-ionized water, sterile
- Duration and temperature of exposure, post-exposure immersion and post-exposure incubation periods (where applicable):
duration:
6 hours incubation
post-incubation:
18 hours
- Number of tissue replicates used per test chemical and controls:
Two tissue samples were used per test chemical and each control
- Wavelength used for quantifying MTT formazan:
570 nm
- Description of the method used to quantify MTT formazan:
The formazan production of the test-substance treated epidermal tissues normalized to that of negative control tissues; the ratio of the values indicates the relative tissue viability
- Historic control data demonstrate reproducibility of results and robustness of the procedures
Results and discussion
In vitro
Results
- Irritation parameter:
- other: tissue viability %
- Value:
- ca. 3
Applicant's summary and conclusion
- Interpretation of results:
- Category 2 (irritating to eyes) based on GHS criteria
- Conclusions:
- Based on the observed results and applying the evaluation criteria it was concluded that the test substance shows an eye irritation potential in the EpiOcular eye irritation test.
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