Registration Dossier
Registration Dossier
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EC number: 254-879-2 | CAS number: 40306-75-0
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 14.06.1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3-acetamido-5-amino-4-hydroxybenzenesulphonic acid
- EC Number:
- 254-879-2
- EC Name:
- 3-acetamido-5-amino-4-hydroxybenzenesulphonic acid
- Cas Number:
- 40306-75-0
- Molecular formula:
- C8H10N2O5S
- IUPAC Name:
- 3-acetamido-5-amino-4-hydroxybenzenesulfonic acid
- Details on test material:
- FAT 92333/A 97.2%
Constituent 1
- Specific details on test material used for the study:
- Code number: FAT - 92'333/A
Batch number: K 37/19.5.92
Purity: 97.2 %
Appearance: solid
Solubility: 1.3 g/l in water
Storage: room temperature
Expiration date: 01.06.93
Toxicity of compounds: IC50 > 320 mg/l
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, adapted
- Details on inoculum:
- Test organism
Strain / Inoculation: Sludge of a biological sewage treatment plant (ARA-Basel Industrie) collected on 13.04.93 - Duration of test (contact time):
- 28 d
- Details on study design:
- Experimental conditions:
Water / Nutrient: according to the specification of the EEC LI 33 (pp. 99-105) Volume 31 resp. OECD Method 3028.
Temperature: 22.0 ± 3 °C (room temperature)
Test duration: 28 days
Lighting: indirect daylight
Estimation Technique: TOC/DOC Analyzer/ SHIMADZU TOC-500
Test concentrations:
150 mg/l DOC nominal
Reference substance
- Reference substance:
- ethylene glycol
- Remarks:
- Diethylenglycol / FAT-90'019/E [Fluka No. 32170]
Results and discussion
% Degradation
- Parameter:
- other: TOC/DOC Analyzer/ SHIMADZU TOC-500
- Value:
- ca. 13.4
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- The bioelimination of the test substance after 28 days is : 13.4 %
The bioelimination of the reference substance after 19 days is : 90.8 %
Any other information on results incl. tables
Test substance
The bioelimination of FAT - 92'333/A measured as DOC after 28 days corrected by the blank control is: 13.4 % (average of 2 tests running in parallel).
Adsorption after 3 hours: 0 %
Reference substance
The bioelimination, corrected by the blank control and measured as DOC (mg/l) is: 90.8 % (19th day)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- not inherently biodegradable
- Executive summary:
The inherent biodegradability of FAT - 92'333/A was determined in a 28 days Zahn-Wellens -Test according to the OECD Guideline for Testing of Chemicals, No. 302B, Paris 1981 and the EEC-Directive, L133 (pp. 99-105) Volume 31, May 88. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland. The test substance was tested in concentration of 150 mg/l DOC. The reference substance Diethylenglycol was also tested in a concentration of 150 mg/l DOC.
The bioelimination of the test substance FAT - 92'333/A after 28 days is: 13.4 %
The bioelimination of the reference substance after 19 days is: 90.8 %
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