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EC number: 603-501-8 | CAS number: 13171-18-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25 January 2019 thru 1 February 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Remarks:
- Extra care taken to minimize volatilization losses
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Reference ID: Propane, 1, 1, 1, 3, 3, 3-hexafluoro-2-methoxy- / CAS No. 13171-18-1
Label ID: 1,1,1,3,3,3,-HEXAFLUORO-2-METHOXYPROPANE-CAS NO.: 13171-18-1
LOT #: 17004
Date & Qty. Received: 06 Nov 18; 65.1 g (GW)
Physical Description: Clear liquid
Storage: Room temperature - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0 and 100 mg/L
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Method: The limit stock solution was prepared by dissolving 370 µL of test substance in 1000 mL of OECD medium and mixing for 15 minutes. This prepared a 200 mg/L solution as the concentration was double the desired final concentration until media containing algae cells was added in equal volume to prepare the 100 mg/L test concentration.
Controls: . A test substance control solution was made using only dilution water. A positive control solution was prepared using zinc chloride (Mfg: Acros, Lot: A037487, Exp: 27 Feb 2022).
Chemical name of vehicle (organic solvent, emulsifier or dispersant): Not applicable
Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): Not applicable
Evidence of undissolved material (e.g. precipitate, surface film, etc.): None - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Green algae
- Strain: Pseudokirchneriella subcapitata (formerly Selenastrum capricornutum)
- Source (laboratory, culture collection): STILLMEADOW, Inc. cultures
- Age of inoculum (at test initiation): 3 - 7 days
-
CULTIVATION METHOD: Pseudokirchneriella subcapitata parent and daughter cultures are continuously maintained at STILLMEADOW, Inc. Prior to testing, 1000 mL of autoclaved OECD medium was inoculated with 5-day old organisms. This new mass culture was then placed in a 24°C environmental chamber. Autoclaved media had a pH of 8.1 ±0.1; therefore, no adjustment to pH was needed. All procedures were conducted aseptically - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- None
- Test temperature:
- 23 - 26 deg C
- pH:
- 7.4 - 9.5
- Nominal and measured concentrations:
- Nominal: 100 mg/L. To account for volatilization losses during preparation and sampling of solutions, nominal dose concentrations were calculated based on an active of 40% concentration and a density of 1.38 g/mL.
Measured (at start): 129.8 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL sterile Erlenmeyer flasks with foam stoppers; filled with 100 mL of the appropriate solution
- Type (delete if not applicable): closed
- Headspace: Minimized due to the substance being highly volatile from water
- Aeration: No
- Type of flow-through (e.g. peristaltic or proportional diluter): Not applicable.
- Renewal rate of test solution (frequency/flow rate): Not applicable.
- Initial cells density: 10000 cells/mL
- No. of organisms per vessel: Not applicable.
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 6 (negative); 3 (positive)
- No. of vessels per vehicle control (replicates): Not applicable.
GROWTH MEDIUM
- Standard medium used: yes
- Detailed composition if non-standard medium was used: Not applicable.
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: continuous
- Light intensity and quality: 4300 lux
- Salinity (for marine algae): Not applicable.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: hemocytometer
- Chlorophyll measurement: Not applicable.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: Not applicable.
- Justification for using less concentrations than requested by guideline: Not applicable.
- Range finding study: Yes, resulting in decision to conduct limit test.
- Test concentrations: 1, 5, 10, 50, 100 mg/L.
- Results used to determine the conditions for the definitive study: Yes - Reference substance (positive control):
- yes
- Remarks:
- Zinc chloride
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 129.8 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no
- Unusual cell shape: no
- Colour differences: no
- Adherence to test vessels: no
- Aggregation of algal cells: no
- Any stimulation of growth found in any treatment: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Reported statistics and error estimates:
- The NOEC and EC50 were determined by a commercially available statistical program (ToxCalc Version 5.0™). Linear Interpolation was used for EC50 determination.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 72-hour EC50 for Pseudokirchneriella subcapitata (Green algae) is >129.8 mg/L.
- Executive summary:
In a GLP guideline study conducted according to OECD Method 201, green algae (Pseudokirchneriella subcapitata) were exposed to the test substance under static conditions in tightly sealed containers at a nominal limit dose concentration of 100 mg/L (measured concentrations at time zero was 129.8 mg/L). No inhibition of growth was observed. Based on these results, the 72 -hour EC50 is >129.8 mg/L and can be considered to not pose a danger in the aquatic environment.
Reference
Limit Test Average Growth Rate Results:
Concentration (mg/L)* |
Rep |
Cell Density x 104cells/mL |
Initial pH |
Terminal pH |
Avg. Growth Rate |
||
24 hours |
48 hours |
72 hours |
|||||
Control |
A |
12 |
28 |
99 |
8.1 |
8.3 |
0.064 |
B |
13 |
33 |
110 |
8.3 |
0.065 |
||
C |
11 |
28 |
97 |
8.5 |
0.064 |
||
D |
10 |
25 |
32 |
7.4 |
NA |
||
E |
10 |
23 |
107 |
8.3 |
0.065 |
||
F |
10 |
19 |
28 |
7.4 |
NA |
||
129.8 |
A |
10 |
11 |
93 |
8.1 |
9.5 |
0.063 |
B |
9 |
25 |
96 |
9.3 |
0.063 |
||
C |
8 |
6 |
21 |
8.2 |
NA |
||
D |
8 |
27 |
101 |
9.5 |
0.064 |
||
Positive Control (10 mg/L) |
A |
0 |
1 |
0 |
7.0 |
7.7 |
0 |
B |
1 |
1 |
1 |
7.7 |
0 |
||
C |
1 |
0 |
0 |
7.6 |
0 |
*Measured.
Description of key information
In a GLP guideline study conducted according to OECD Method 201, green algae (Pseudokirchneriella subcapitata) were exposed to the test substance under static conditions in tightly sealed containers at a nominal limit dose concentration of 100 mg/L (measured concentrations at time zero was 129.8 mg/L). No inhibition of growth was observed. Based on these results, the 72 -hour EC50 is >129.8 mg/L and can be considered to not pose a danger in the aquatic environment.
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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