Divanadyl pyrophosphate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

31.08.1988 - 14.09.1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets generally accepted scientific standards, well documented and acceptable for assessment.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EPA OPP 81-2 (Acute Dermal Toxicity)

GLP compliance:

yes

Test type:

other: Limit-test

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Divanadyl pyrophosphate

EC Number:

407-130-0

EC Name:

Divanadyl pyrophosphate

Cas Number:

65232-89-5

Molecular formula:

(VO)2P2O7

IUPAC Name:

(phosphonooxy)phosphonic acid dihydrate vanadium

Details on test material:

- Physical state: greyish-green powder
- Analytical purity: 100%
- Lot/batch No.: 3883559 A
- Storage condition of test material: room temperature

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Hazleton-Dutchland Laboratory Animals, Denver, Pennsylvania.
- Age at study initiation: young adults (at least 8 weeks old).
- Weight at study initiation: males: 2,5 - 2,7 kg; females: 2,7 - 2,9 kg.
- Housing: individually.
- Diet: Lab Rabbit Chow HF (Purina #5326), 125 g/day while on test.
- Water: tap water, ad libitum.
- Acclimation period: 44 days.

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 16 - 21 °C
- Humidity (%): 30 - 70 %
- Photoperiod (hrs dark / hrs light): 12 h / 12 h

Administration / exposure

Type of coverage:

occlusive

Vehicle:

physiological saline

Details on dermal exposure:

TEST SITE
- Area of exposure: 4" x 12"
- Type of wrap if used: impervious plastic sheet

REMOVAL OF TEST SUBSTANCE
- Washing (if done): only wiped free of excess test material with dry paper towels.
- Time after start of exposure: 24 h

TEST MATERIAL
- For solids, paste formed: the dry material was placed direct on a gauze and moistened with 7 mls of saline

VEHICLE
- Amount(s) applied (volume or weight with unit): 7 ml

Duration of exposure:

24 h

Doses:

5000 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days.
- Frequency of observations: twice daily.
- Frequency of weighing: pretest (time of clipping), predose, days 7 and 14.
- Necropsy of survivors performed: yes.
- Other examinations performed: clinical signs, body weight.

Statistics:

no statistics performed.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Mortality:

No mortality occured.

Clinical signs:

other: Local signs: Two males exhibited severe dermal effects at a small area of the dose site (necrosis followed by eschar formation, and exfoliation of the eschar tissue) which persisted throughout the study. Systemic toxicity: All animals were free of signi

Gross pathology:

Gross postmortem observations confirmed the presence of dermal lesions. Other observations were similar to those seen in control animals or were considered to represent normal physiological variation.