C.I. Solvent Red 119

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May 2017 - October 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

C.I. Solvent Red 119

EC Number:

602-676-8

Cas Number:

12237-27-3

Molecular formula:

C32H22N10O8Cr

IUPAC Name:

C.I. Solvent Red 119

impurity 1

Reference substance name:

Unknown impurities

Cas Number:

not available

IUPAC Name:

Unknown impurities

impurity 2

Reference substance name:

Water

EC Number:

231-791-2

EC Name:

Water

Cas Number:

7732-18-5

Molecular formula:

H2O

IUPAC Name:

water

Test material form:

solid: particulate/powder

Study design

Inoculum or test system:

other: activated sludge, biologic sewage treatment plant

Results and discussion

% Degradation

Key result

Parameter:

% degradation (CO2 evolution)

Value:

ca. 3

Sampling time:

28 d

Remarks on result:

not readily biodegradable based on QSAR/QSPR prediction

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

All validity criteria were met.
Degradation behaviour of positive control and toxicity control was normal. Abiotic degradation
was not observed. Both replicates of the test item showed good correspondence.
If degradation in the toxicity flask is below 25 % after 14 days, the test item can be considered
as toxic towards the inoculum. As degradation in the toxicity flask was 39.9 % after
14 days, the test item can be stated as “not toxic towards the inoculum in a concentration
of 42.1 mg/L”.
Ready degradability is defined in the guidelines as degradation surpassing 60% within 10
days after reaching a level of 10 %.
For the test item Solvent Red 119 only 3 % biodegradation was observed after 28 days.
The 10-day window could not be determined. The criterion of reaching 60% of degradation
is not met. Solvent Red 119 is therefore considered as “not readily biodegradable”.
Degradation missed 60% within 28 days, though. Therefore, the test item is considered as
“ not ultimately biodegradable within 28 days”, either.
No observations were made which might cause doubts concerning the validity of the study
outcome.
The result of the test can be considered valid.

Executive summary:

The test item Solvent Red 119 was tested using a concentration of nominally 20 mg organic

carbon/L (corresponding to 41.9 mg Solvent Red 119/L) in test medium following OECD

301B and EU-Method C.4-C.

Aniline was chosen as positive control.

Activated sludge was used as inoculum (concentration in the test 25.0 mg dry matter/L).

The test was left running for 28 days.

All validity criteria were met. Degradation of the positive control was 68 % after 8 days.

The following data were determined for the test item Solvent Red 119:

10-day-window: not detected

degradation at the end of 10-day-window: none

degradation at the end of the test: 3 %

pass level following guideline: 60 % at the end of 10-day-window for pure substances

respective 60 % at the end of the test for mixtures

For the test item Solvent Red 119 only 3 % biodegradation was observed after 28 days.

The 10-day window could not be determined. The criterion of reaching 60% of degradation

after 28 days is not met. Solvent Red 119 is therefore considered as “not readily biodegradable”.

As degradation missed 60% in the course of the test, Solvent Red 119 is considered as

not ultimately biodegradable within 28 days.