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EC number: 909-129-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 4 November 1994 - 18 November 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Version / remarks:
- 17 July 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.4 (Acute Toxicity: Dermal Irritation / Corrosion)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- dd. 16 March 1994
Test material
- Reference substance name:
- m,α-dimethylstyrene
- EC Number:
- 214-394-9
- EC Name:
- m,α-dimethylstyrene
- Cas Number:
- 1124-20-5
- Molecular formula:
- C10H12
- IUPAC Name:
- 1-isopropenyl-3-methylbenzene
- Test material form:
- liquid
- Details on test material:
- Indentification: m/p- Isopropenyl toluene
Appearance: clear colourless liquid
Storage condition: room temperature
Purity: Not specified
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- - Source: David Percival Ltd., Moston, Sandbach, Cheshire U.K.
- Age at study initiation: twelve to sixteen weeks old
- Weight at study initiation: 2.32 - 2.65 kg
- Housing: Animals were housed individually in suspended metal cages
- Diet: Free access to diet (STANRAB SQC Rabbit Diet, Special Diets Services Ltd., Witham, Essex, U.K.)
- Water: Free access to tap water.
- Acclimation period: At least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 16 – 19
- Humidity (%): 56 - 65
- Air changes (per hr): approx 15
- Photoperiod (hrs dark / hrs light): 12/12
Test system
- Type of coverage:
- semiocclusive
- Preparation of test site:
- clipped
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: Adjacent areas of the untreated skin of each animal served as controls.
- Amount / concentration applied:
- TEST MATERIAL
- Amount applied: 0.5 mL - Duration of treatment / exposure:
- 4 hours
- Observation period:
- 14 days
- Number of animals:
- 1 male and 2 females
- Details on study design:
- Study Design:
On the day before application the rabbits were clipped free of fur from the dorsal flank area using veterinary clippers. Only animals with a healthy intact epidermis by gross observation were selected for the study. On the day of application a suitable test site was selected on the back of each rabbit. A quantity of 0.5 mL of the test material was applied to the skin under a 2.5 cm x 2.5 cm cotton gauze patch. The patch was secured in position with a strip of surgical adhesive tape (BLENDERM: approximate size 2.5 cm x 4.0 cm). Thereafter the trunk of each rabbit was wrapped in an elasticated corset (TUBIGRIP).
Removal of test substance.
Four hours after application, the test material was removed by gentle swabbing with cotton wool soaked in 74% Industrial Methylated Spirits.
Observations:
Skin reactions were assessed at approximately 1, 24, 48, 72 hours and 7 and 14 days after the removal of the dressings and test substance.
Other effects to the skin were also recorded.
Scoring system:
Draize scale
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 2
- Max. score:
- 4
- Reversibility:
- fully reversible within: 7 days
- Irritation parameter:
- erythema score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 2
- Max. score:
- 4
- Reversibility:
- fully reversible within: 14 days
- Irritation parameter:
- erythema score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 2
- Max. score:
- 4
- Reversibility:
- fully reversible within: 7 days
- Irritation parameter:
- edema score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 2
- Max. score:
- 4
- Reversibility:
- fully reversible within: 7 days
- Irritation parameter:
- edema score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 3.3
- Max. score:
- 4
- Reversibility:
- fully reversible within: 7 days
- Irritation parameter:
- edema score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 3
- Max. score:
- 4
- Reversibility:
- fully reversible within: 7 days
- Irritant / corrosive response data:
- Well-defined erythema was noted at all treated skin sites one hour after patch removal and up to the 72 hour observation time point. The erythema extended up to 5 cm beyond all treatment sites throughout this observation period
Moderate to severe oedema was noted at all treated skin sites one hour after patch removal with slight to severe oedema at the 24-hour observation point and slight to moderate oedema at the 48 and 72-hour observation points. The oedema extended ventrally below one treatment site up to the 48 hour observation time point. - Other effects:
- Crust formation extending up to 5 cm beyond all treatment sites was observed in all animals at the 7 day observation time point. Crust formation prevented accurate evaluation of erythema at one treated skin site after 7 days. Desquamation or crust formation was observed at all treated sites at the 14 day time point.
Applicant's summary and conclusion
- Interpretation of results:
- Category 2 (irritant) based on GHS criteria
- Conclusions:
- An acute dermal irritation study was performed according to OECD guideline 404 and EU method B.4. A. Based on the results, m/p - isopropenyl toluene is classified as skin irritant (category 2) according to GHS and CLP criteria.
- Executive summary:
m/p - Isopropenyl toluene was evaluated for skin irritation on 2 female and 1 male New Zealand White rabbits according to OECD test guideline 404 and EU method B.4 under GLP principles.
0.5 mL was applied semi-occlusively for 4 hours to clipped rabbit skin. Observations took place 1, 24, 48, 72 hours and 7 and 14 days after the removal of the test substance. Well-defined erythema and slight to severe oedema was observed until the 72 hour observation point. Mean scores for erythema of the 24h 48h 72h observation time points were 2.0 in all animals and mean scores for oedema were 2.0, 3.3 and 3.0. Oedema showed reversibility within 7 days and erythema was reversible within 14 days. Crust formation was observed 7 days after removal of the test substance and desquamation or crust formation was observed 14 days after removal of the test substance. Based on these results m/p - Isopropenyl toluene is classified as a skin irritant category 2 (H315) according to the Globally Harmonized System of Classification and Labelling of Chemicals (GHS) of the United Nations (2015) (including all amendments) and Regulation (EC) No 1272/2008 on classification, labelling and packaging of items and mixtures (including all amendments).
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