Methyl 2-((allyloxy)methyl)acrylate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

9th December 2013 - 13th December 2013

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations:
range finding test - 1, 10, 100 mg/L,
main test - 1, 1.8, 3.2, 5.6, 10mg/L
- Sampling method: Samples of the test solutions at the beginning and at the end of the renewal period, i.e. when it is freshly prepared and immediately prior to renewal or at the end of exposure are referred to as one set. The two sets of test solutions, one at the beginning of exposure (0 hour) and before renewal at 24 hours and the other after renewal at 72 hours and at the end of exposure (96 hours), were analyzed.
- Sample storage conditions before analysis: stored in a refrigerator.

Vehicle:

no

Details on test solutions:

To maintain the concentration of the test substance, test solutions were renewed every 24-hour. Moreover, the surface of the test solution was covered with a Teflon sheet to prevent evaporation of the test solution and volatilization of the test substance. Renewal of the test solutions was performed by transferring the fish to freshly prepared test solution.

Test organisms (species):

Oryzias latipes

Details on test organisms:

Source: Tsunashima Fishing Co. (18-1 Tsunashima-nishi 5-chome, Kouhoku-ku,
Yokohama, Kanagawa, Japan)
4) Date of receipt:October 23, 2013
5) Lot No.:13-H-1023
6) Size:
Total length: Recommended: 2.0 ± 1.0 cm
Measurements: 2.69 cm (2.30-2.96 cm), n=10
Body weight: Measurements: 0.184 g (0.135-0.284 g), n=10
(These values were measured and weighed for the fish of control at the end of exposure.)
Age: Less than a half year after hatching.
Acclimation period: December 2, 2013 -December 9, 2013
Acclimation conditions:
Water: Dechlorinated water
Procedure:Flow-through (Biomass loading: l g fish/L/day or less)
Temperature:24 ± 1°c
Dissolved oxygen concentration (D.0.): 80% or more of the saturation
pH: 6.0-8.5
Light: Fluorescent light, 16-hour light (1000 lux or less)/ 8-hour dark
Feed: TetraMin, TetraWerke
Feeding amount: 2% of the body weight/day (approx.) (until 24 hours before exposure)
Medication during acclimation: Not applied
Mortality during 7 days before the test: Less than 5%

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

Test fish were observed at 3, 24, 48, 72 and 96 hours after the start of exposure.

Hardness:

242 mg CaCO3/L

Test temperature:

24°C

pH:

Not adjusted

Dissolved oxygen:

60% or more of the saturation (not aerated)

Nominal and measured concentrations:

Nominal: 1, 1.8, 3.2, 5.6, 10 mg/L
Mean measured: 1.04, 1.80, 3.23, 5.76 and 10.3 mg/L

Details on test conditions:

Volume of test solution: 5.0 L/vessel (The measurement samples of water quality and analysis were collected from each vessel; kept biomass loading: 1.0g fish/L or less).
Number of vessels: 1 vessel/test group
Biomass loading:10 fish/test group
Light:Fluorescent light, 16-hour light (1000 lux or less)/ 8-hour dark
Feeding: None

Reference substance (positive control):

yes

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

2.56 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Abnormal symptom (abnormal swimming) was observed in Conc.2 to Conc.4, impossible
to swim was observed in Conc.5, and dead fish were observed in Conc. 3 to 5. In control
groups, the mortalities at 96-hour exposure was 0%. In Cone. I to 5, those were 0, 0, 90, 100
and 100%, respectively.

Validity criteria fulfilled:

yes

Conclusions:

96 hour LC50: 2.56mg/L
96 hour LC0: 1.80mg/L
96 hour LC100: 5.76mg/L

Description of key information

96 hour LC50: 2.56mg/L
96 hour LC0: 1.80mg/L
96 hour LC100: 5.76mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

EC50

Effect concentration:

2.56 mg/L

Additional information

A determination of the acute toxicity to fish of 2-(Allyloxymethyl) acrylic acid methyl ester was conducted (Mitsubishi Chemical Medience Corporation, 2014, Study A130915).
The study was performed in accordance with OECD test guideline 203, and in compliance with GLP.

Groups of 10 fish were exposed to 0 (control group), 1.0, 1.8, 3.2, 5.6, or 10 mg/L 2-(Allyloxymethyl) acrylic acid methyl ester. Test fish were observed at 3, 24, 48, 72 and 96 hours after the start of exposure.
Abnormal symptoms (abnormal swimming) was observed in Conc.2 to Conc.4, impossible to swim was observed in Conc.5, and dead fish were observed in Conc. 3 to 5.

It was concluded that the 96-hour EC50 in Fish was 2.56 mg/L and EC0 in Fish was 1.80 mg/L