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EC number: 223-211-1 | CAS number: 3770-97-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- October 31, 1996 - November 27, 1996
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Version / remarks:
- 02-1987
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.1 (Acute Toxicity (Oral))
- Version / remarks:
- 07-1992
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- 6-diazo-5,6-dihydro-5-oxonaphthalene-2-sulphonyl chloride
- EC Number:
- 223-211-1
- EC Name:
- 6-diazo-5,6-dihydro-5-oxonaphthalene-2-sulphonyl chloride
- Cas Number:
- 3770-97-6
- Molecular formula:
- C10H5ClN2O3S
- IUPAC Name:
- 6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalene-2-sulfonyl chloride
- Test material form:
- solid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Remarks:
- Hoe:WISKf(SPF71)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Hoechst AG, Kastengrund, Germany
- Females (if applicable) nulliparous and non-pregnant: yes
- Age at study initiation: 7 - 8 weeks
- Weight at study initiation: 151 - 187 g
- Fasting period before study: From about 16 hours before dosing to 3-4 hours after treatment
- Housing: groups of 5 in type 4 macrolon cages
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: at least 1 day
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 3
- Humidity (%): 50 +/- 20
- Photoperiod (hrs dark / hrs light): 12 / 12
IN-LIFE DATES: From days 1 to 15
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- DMSO
- Details on oral exposure:
- Doses were based on a range finding study.
Dosing volume: 10 mL/kg. - Doses:
- 1250, 1600 and 2000 mg/kg bw
- No. of animals per sex per dose:
- 1250 mg/kg: 5 m / 5 f
1600 mg/kg: 5 f
2000 mg/kg: 5 m / 5 f - Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: once or twice daily
- Frequency of weighing: weekly
- Necropsy of survivors performed: yes (gross pathology) - Statistics:
- Standard statistical methods have been applied for data processing.
Results and discussion
- Preliminary study:
- A range finding was performed.
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 1 250 - < 1 600 mg/kg bw
- Based on:
- test mat.
- Mortality:
- 1250 mg/kg: 1/5 m, 1/5 f
1600 mg/kg: 5/5 f
2000 mg/kg: 5/5 m, 5/5 f - Clinical signs:
- Decreased spontaneous activity, squatting posture, sunken flanks, bristled coat, irregular respiration, panting, stupor, prone position, narrowed palpebral fissures, increased salivation and diarrhoea.
All clinical signs had reversed in the surviving animals at day 4 of the study. Additionally the bedding of the cages was discoloured yellow up to day two of the study. - Body weight:
- Surviving animals showed expected gain in bodyweight during the study.
- Gross pathology:
- Animals found dead:
Stomach: full of test compound, full of a reddish-black mass, Haemoccult test positive, mucosa discoloured black.
Intestinal tract: full of a reddish-black mass, Haemoccult test positive, mucosa of the small intestine diffuse reddened, small intestine full of a yellowish mucous.
Abdominal cavity: full of a clear, colourless fluid.
Connective and supportive tissue: discoloured by the test compound.
The animals killed at the end of the observation period showed no macroscopically visible changes.
Any other information on results incl. tables
Study design
The test item diluted in DMSO was tested for acute toxicity in rats after single oral administration of 1250 - 2000 mg/kg body weight. This GLP study was performed according to the OECD GL 401. The surviving animals were observed for fourteen days after the day of dosing and were then killed for gross pathological examination.
Results
At dose levels of 1250, 1600 and 2000 mg/kg the mortality was 1/5 m and 1/5 f, 5/5 f or 5/5 m and 5/5 f, respectively. Clinical signs of intoxication included decreased spontaneous activity, squatting posture, sunken flanks, bristled coat, irregular respiration, panting, stupor, prone position, narrowed palpebral fissures, increased salivation and diarrhoea. All clinical signs had reversed in the surviving animals at day 4 of the study. Additionally the bedding of the cages was discoloured yellow up to day two of the study. Surviving animals showed expected gain in bodyweight during the study.
Abnormalities noted at necropsy of rats that died during the study were effects on the stomach (full of test compound, full of a reddish-black mass, Haemoccult test positive, mucosa discoloured black), intestinal tract (full of a reddish-black mass, Haemoccult test positive, mucosa of the small intestine diffuse reddened, small intestine full of a yellowish mucous), abdominal cavity (full of a clear, colourless fluid), and connective and supportive tissue (discoloured by the test compound). No abnormalities were noted at necropsy of animals killed at the end of the study.
Conclusion
For regulatory purposes, the median lethal dose (LD50) can be declared in the range of 1250 to 1600 mg/kg.
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- For regulatory purposes, the median lethal dose (LD50) can be declared in the range of 1250 to 1600 mg/kg.
- Executive summary:
This study was performed according to GLP and is fully compliant with OECD GL 401. Based on the result of this study, the median lethal dose (LD50) can be declared in the range of 1250 to 1600 mg/kg.
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