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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
07 Dec 1983
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Qualifier:
according to guideline
Guideline:
other: ETAD 103
GLP compliance:
not specified
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
activated sludge
Details on inoculum:
Mixed culture of bacteria (sludge from Husman apparatus)
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Test temperature:
18 - 22°C
Nominal and measured concentrations:
Nominal: 1.0, 3.2, 10, 32, 100 mg/L
Details on test conditions:
Measured endpoint: O2 consumption (measured with oxygen-sensitive electrode system)
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol
Duration:
3 h
Dose descriptor:
IC10
Effect conc.:
ca. 10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of heterotrophic respiration
Duration:
3 h
Dose descriptor:
IC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of heterotrophic respiration
Results with reference substance (positive control):
IC50: 19 mg/L corresponding to a normal sensitivity of the test system (range 10-25 mg/L)
Validity criteria fulfilled:
yes
Conclusions:
The substance caused a IC50 (inhibition of heterotrophic respiration) of >100 mg/L. The IC10 after 3 h was ca. 10 mg/L in this OECD 209 guideline study. The substance is not considered inhibitory to activated sludge when applied at normally low concentrations.

Description of key information

The substance caused a IC50 (inhibition of heterotrophic respiration) of >100 mg/L. The IC10 after 3 h was ca. 10 mg/L in this OECD 209 guideline study. The substance is not considered inhibitory to activated sludge when applied at normally low concentrations.

Key value for chemical safety assessment

EC50 for microorganisms:
100 mg/L
EC10 or NOEC for microorganisms:
10 mg/L

Additional information