Cyclododeca-1,5,9-triene

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study without detailed documentation

Data source

Reference

Reference Type:

other: J-CHECK databank

Title:

Information on BCF tests done by METI for EU PBT candidate substances, 07 October 2003

Author:

METI (Ministry of Environment, Trade and Industries)

Year:

2003

Bibliographic source:

METI, Japan 12 pp

Report date:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)

GLP compliance:

no

Test material

Test material information

Constituent 1

Details on test material:

IUCLID4 Test substance: other TS: 1,5,9-cyclododecatriene, purity 94 % (trans, trans, trans form)
Origin: Tokyo Kasei Kogyo Co., Ltd.

Radiolabelling:

no

Sampling and analysis

Details on sampling:

- Sampling in weeks 1, 2, 4, 6, 8 and 10 (both fish and water)

Test solutions

Vehicle:

yes

Details on preparation of test solutions, spiked fish food or sediment:

- Vehicle / dispersant: HCO-20, HCO-40

Test organisms

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISMS
- Size of fish: average weight 26.2 g, average total length 9.8 cm
- Lipid content of test organism: 5 % average

Study design

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

70 d

Test conditions

Test temperature:

25 °C +/- 1 °C

Details on test conditions:

EXPOSURE CONDITIONS
- Exposure vessel: 100 l glass tank, 1155 l/day flow-through

- Results based on measured concentrations in water

Nominal and measured concentrations:

Concentration of test substance: 1 µg/l, 10 µg/l (nominal)

Reference substance (positive control):

no

Results and discussion

Bioaccumulation factor

Bioaccumulation factor 1

Type:

BCF

Value:

>= 2 630 - <= 12 500 L/kg

Basis:

whole body w.w.

Remarks on result:

other: Conc.in environment / dose:10 µg/L

Bioaccumulation factor 2

Type:

BCF

Value:

>= 1 920 - <= 14 800 L/kg

Basis:

whole body w.w.

Remarks on result:

other: Conc.in environment / dose:1 µg/L

Depuration

Elimination:

no

Any other information on results incl. tables

Test concentration (nominal)		Test duration
		1 week	2 weeks	4 weeks	6 weeks	8 weeks	10 weeks
10 µg/L	Measured test concentration (µg/L)	5.54	5.59	6.12	6.49	6.90	6.95
	BCF (L/kg): replicate 1	2690	5140	4840	12500	9750	5560
	BCF (L/kg): replicate 2	3370	2630	8070	10800	8850	8960
1 µg/L	Measured test concentration (µg/L)	0.442	0.511	0.576	0.617	0.650	0.666
	BCF (L/kg): replicate 1	1920	4590	4080	5610	9270	10300
	BCF (L/kg): replicate 2	2950	2450	3240	4830	14800	6220