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Diss Factsheets
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EC number: 204-310-9 | CAS number: 119-27-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Dermal absorption
Administrative data
- Endpoint:
- dermal absorption in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 01 December 2011 - 31 July 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 428 (Skin Absorption: In Vitro Method)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2,4-dinitroanisole
- EC Number:
- 204-310-9
- EC Name:
- 2,4-dinitroanisole
- Cas Number:
- 119-27-7
- Molecular formula:
- C7H6N2O5
- IUPAC Name:
- 2,4-dinitroanisole
Constituent 1
Test animals
- Species:
- other: human
- Details on test animals or test system and environmental conditions:
- N/A
Administration / exposure
- Type of coverage:
- open
- Vehicle:
- unchanged (no vehicle)
- Duration of exposure:
- 8 hours
- Doses:
- - Actual doses: 100 mg
- No. of animals per group:
- 1
- Control animals:
- no
- Details on study design:
- TEST SITE
- Coverage: 0.64 cm2 - Details on in vitro test system (if applicable):
- SKIN PREPARATION
- Source of skin: abdominal human skin (from autopsy or surgery)
- Ethical approval if human skin: not specified
- Preparative technique:
Full thickness abdominal human skin (from autopsy or surgery) was obtained from the National Disease Research Interchange (NDRI), Philadelphia, Pennsylvania. The whole skin was stored frozen at -85 °C. Before starting the experiments, the skin was placed in a Petri dish containing a buffer solution at room temperature. The subcutaneous fat was removed with blunt forceps, and the skin was cleaned with a buffer solution and placed on a dissecting board. Circular pieces of skin sections (0.64 cm2) were prepared from the skin with the help of a metallic Arch Punch (18 mm; Osborne and Company). The epidermis was teased from dermis as described by Frasch et al. 2011. The skin discs were wrapped in Saran® Wrap and submerged in a 60°C buffer for approximately 70 seconds. The skin was unwrapped and placed in a Petri dish containing a buffered solution. The dermis of the skin discs was teased from the epidermis carefully using blunt forceps or cotton swabs under a binocular microscope. Care was taken not to damage the skin. During epidermal membrane separation, a thin layer of stratum corneum was easily separated from the skin. The epidermal membranes remaining were used for testing. The epidermal membranes were evaluated under a dissection microscope to ensure that they were free of any damage. The epidermal membranes (without stratum corneum) were placed on wax paper moistened with a buffered solution and frozen (-30°C) prior to experimental use.
Results and discussion
Percutaneous absorption
- Key result
- Time point:
- 8 h
- Dose:
- 100 mg
- Parameter:
- rate
- Absorption:
- ca. 0.002 %
Applicant's summary and conclusion
- Conclusions:
- The analysis of the absorbed chemical in the receptor fluid data showed that steady state fluxes to an infinite dose (100 milligrams (mg)) of neat DNAN was 1.1 μg/cm2 /hr.
The cumulative dermal absorption of DNAN over 8 hours was 12.5 μg/cm2
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