4-trans-Butyl-4'-trans-propyl-[1,1'-bicyclohexyl]

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

09/05/2007-12/08/2009

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-trans-Butyl-4'-trans-propyl-[1,1'-bicyclohexyl]

EC Number:

619-232-4

Cas Number:

96624-52-1

Molecular formula:

C36H36

IUPAC Name:

4-trans-Butyl-4'-trans-propyl-[1,1'-bicyclohexyl]

Test material form:

other: waxy mass

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

Macro nutrients (mg/L)
CaCI2 •2H20 293.80
MgSO4•7H20 123.30
NaHCO3 64.80
KCI 5.80
Na2SiO3•9H20 10.00
NaNO3 0.27
KH2PO4 0.14
K2HPO4 0.18

Trace elements mg/L
B 0.5000
Fe 0.2000
Mn 0.1000
Li, Rb and Sr 0.0500
Mo 0.0250
Br 0.0125
Cu and Zn 0.0063
Co and I 0.0025
Se 0.0010
V 0.0003

Macro nutrients
Na2EDTA • 2H20 2.50

Vitamins (µg/L):
Thiamine 75.00
B12 1.00
Biotin 0.75

- pH: 8 ± 0.3
- hardness 14 ° dH (250 mg/L CaCO3)

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia
- Strain/clone: Daphnia Magna
- Source: in house
- Age of parental stock: younger than 24 hours
- Feeding during test: no


ACCLIMATION
- Acclimation period: 5 days
- Acclimation conditions: same as test (controlled environment room, pH 7.9-8.0, 370-400 lux, 16h light / 8 h dark)
- Type and amount of food: green algea (Scenedesmus subspicatus)
- Health during acclimation : no mortality observed


At the start of the experimental phase 5 Daphnia magna were placed in each test vessel containing 10 ml reconstituted water with or without test material. The test vessels were left open.

Study design

Test type:

other: open-static

Water media type:

freshwater

Total exposure duration:

48 h

Test conditions

Hardness:

14o dH (about 250 mg/L CaCO3)

Test temperature:

19 – 20°C

pH:

7.9 +/- 0.3

Dissolved oxygen:

at least 90 %

Nominal and measured concentrations:

Nominal 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: open static
- Material: test item in reconstituted water
- Aeration: 24 hours after preparation
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
- Biomass loading rate: --


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted according to ELENDT (1990)



Macro nutrients mg/L
CaCl2 x 2H2O 293.80
MgSO4 x 7H2 123.30
NaHCO3 64.80
KCl 5.80
Na2SiO3 9H2O 10.00
NaNO3 0.27
KH2PO4 0.14
K2HPO4 0.18


Trace elements mg/L
B 0.5000
Fe 0.2000
Mn 0.1000
Li, Rb and Sr 0.0500
Mo 0.0250
Br 0.0125
Cu and Zn 0.0063
Co and I 0.0025
Se 0.0010
V 0.0003
Macro nutrients
Na2EDTA  2H2O 2.50

Vitamins µg/L
Thiamine 75.00
B12 1.00
Biotin 0.75


- Total organic carbon: --
- Particulate matter: --
- Metals: --
- Pesticides: --
- Chlorine: --
- Alkalinity: --
- Ca/mg ratio: --
- Conductivity: --
- Culture medium different from test medium: no
- Intervals of water quality measurement: 48 h


OTHER TEST CONDITIONS
- Adjustment of pH: 7.9
- Photoperiod: 16h light / 8h dark
- Light intensity: --


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :


TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:

References:

ELENDT, B.-P. Selenium deficiency in Crustacea. An ultrastructural approach to antennal damage in Daphnia magna Straus.
Protoplasma 154, 25-33, 1990

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

In this open static test system, Daphnia magna exposed to a nominal concentration of 100 mg/L revealed no biological effects.

Results with reference substance (positive control):

Potassium dichromate:
24 h EC50 0.95 (0.87-1.04) mg/L
48 h EC50 0.74 (0.68-0.81) mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the given experimental conditions, the test material showed no aquatic toxicity. The 48 hours NOEC to Daphnia magna is > 100 mg/L.