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EC number: 269-044-8 | CAS number: 68186-64-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2019-05-13 to 2019-07-18
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Remarks:
- LC/MS/MS
- Details on sampling:
- For determination of the test item concentrations, five replicate samples were taken from the test solution and five replicate samples from the control at the start and at the end of the test. The sampling was performed from different part of the aquariums.
- Vehicle:
- no
- Details on test solutions:
- The test solution was prepared by mechanical dispersion without using any solubilising agent. An amount of 0.5008 g test item was dissolved in 5008 mL ISO medium, resulting a nominal concentration of 100 mg/L. The test solution was freshly prepared in the testing laboratory just before introduction of fish (start of experiment).
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish (Danio rerio)
- Source: Akvárium Magazin Kft. (Pasaréti Gyula), 1222 Budapest, Dévény u. 36., Hungary
- Age at study initiation: juveniles
- Length at study initiation: 2.00 - 2.50 cm
- Weight at study initiation: 0.163 - 0.197 g
ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions: same as test condition
- Type and amount of food during acclimation: commercial diet for fish
- Feeding frequency during acclimation: at least three times per week until 24 hours before the treatment
- Health during acclimation (any mortality observed): no mortality occured in 7 days before the start of the test
FEEDING DURING TEST
- Food type: no feeding during test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 249 mg/L (as CaCO3)
- Test temperature:
- 21.8 - 22.3 °C
- pH:
- 7.48 - 7.84
- Dissolved oxygen:
- 63.2 - 86.9 %
- Nominal and measured concentrations:
- Nominal concentration: 100 mg/L
Measured concentration: at the start - 91 mg/L, at the end - 86 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: aquariums
- Type: open
- Material, size, headspace, fill volume: glass, 5 L testing solution
- Aeration: none, in the treatment group at the 72th hour slight aeration was applied to contain the dissolved oxygen level above 60 %
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.33 - 0.39 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO medium, prepared according to OECD 203
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 h light : 8 h darkness
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 100 mg/L
- Results used to determine the conditions for the definitive study: no mortality occured during the exposure period - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- There was no mortality observed in the 10 fish in the control group or in the 10 fish exposed to the test item concentration of 100 mg/L. No sub-lethal effect was observed in the control and in the test item treated group during the study.
The 96-h No Observed Effect Concentration (NOEC) was determined to be 100 mg/L. The 96-h LC50, LC100 and the LOEC values were determined to be > 100 mg/L, the 96-h LC0 value was determined to be 100 mg/L based on nominal test concentration. - Sublethal observations / clinical signs:
Analytical Results
A single test item concentration of 100 mg/L (limit test) and a concurrent control were tested in the main test. The concentration of the test item was analytically determined in the test item solution and in the control at the start and at the end of the experiment. The mean of the measured concentrations of the test items main component was 91 mg/L at the start and 86 mg/L at the end of the test in the test item treated group. The measured test item concentration remained within ± 20 % of the nominal during the test period of 96 hours. Therefore, the biological results are based on the nominal test item concentration. Interfering component was not detected in the control samples.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this 96-hour acute toxicity test on Zebrafish (Danio rerio) the test item had no toxic effect on fish up to the limit concentration of 100 mg/L. Accordingly, the 96-h LC50 value was determined to be > 100 mg/L. The 96-h NOEC was determined to be 100 mg/L.
- Executive summary:
The acute toxicity of the test item to fish (Zebrafish) was determined according to the OECD Guideline 203, EU Council Regulation No. 440/2008 method C.1 and EPA OPPTS 850.1075. A static test was performed as a limit test with a test concentration of 100 mg/L. The test item solution was prepared by mechanical dispersion – 0.5008 g test item were dissolved in 5008 mL ISO medium. Ten test organisms were exposed to the test concentration and the control, respectively, for 96 hours. The measured concentration of the test item at the start of the test was 91 mg/L and at the end of the test was 86 mg/L, respectively. The measured test item concentration remained within ± 20 % of the nominal during the test period of 96 hours. Therefore, the biological results are based on the nominal test item concentration. In conclusion, the test item had no toxic effect on fish up to the limit concentration of 100 mg/L. Accordingly, the 96-h LC50 value was determined to be > 100 mg/L. The 96-h NOEC was determined to be 100 mg/L.
Reference
Description of key information
In a 96-hour acute toxicity test on Zebrafish (Danio rerio) the test item had no toxic effect on fish up to the limit concentration of 100 mg/L. Accordingly, the 96-h LC50 value was determined to be > 100 mg/L. The 96-h NOEC was determined to be 100 mg/L.
Key value for chemical safety assessment
Additional information
The acute toxicity of the test item to fish (Zebrafish) was determined according to the OECD Guideline 203, EU Council Regulation No. 440/2008 method C.1 and EPA OPPTS 850.1075. A static test was performed as a limit test with a test concentration of 100 mg/L. The test item solution was prepared by mechanical dispersion – 0.5008 g test item were dissolved in 5008 mL ISO medium. Ten test organisms were exposed to the test concentration and the control, respectively, for 96 hours. The measured concentration of the test item at the start of the test was 91 mg/L and at the end of the test was 86 mg/L, respectively.The measured test item concentration remained within ± 20 % of the nominal during the test period of 96 hours. Therefore, the biological results are based on the nominal test item concentration.In conclusion,the test item had no toxic effect on fish up to the limit concentration of 100 mg/L. Accordingly, the 96-h LC50 value was determined to be > 100 mg/L. The 96-h NOEC was determined to be 100 mg/L.
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