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EC number: 247-556-2 | CAS number: 26264-05-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Dodecylbenzenesulphonic acid, compound with isopropylamine (1:1)
- EC Number:
- 247-556-2
- EC Name:
- Dodecylbenzenesulphonic acid, compound with isopropylamine (1:1)
- Cas Number:
- 26264-05-1
- Molecular formula:
- C18H30O3S.C3H9N
- IUPAC Name:
- sodium dodecylbenzenesulfonate
- Test material form:
- liquid
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: microbial activity introduced by inoculation with secondary effluent
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): The source of test organisms was secondary effluent freshly obtained from a municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
- Storage length: Not specified
- Preparation of inoculum for exposure: Not specified
- Pretreatment: Secondary effluent was filtered through a coarse filter paper, the first 200 mL were discarded. The filtrate was kept aerated until inoculation.
- Concentration of sludge: 4 mL filtrate of secondary effluent per litre of final volume.
- Water filtered: yes
- Type and size of filter used, if any: Coarse filter paper - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 1.5 mg/L
- Based on:
- ThOD
- Initial conc.:
- 4 mg/L
- Based on:
- ThOD
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Standard as per OECD guideline
- Additional substrate: Not specified
- Solubilising agent (type and concentration if used): None
- Test temperature: 22 to 23°C
- pH: between 7.4 and 7.6 at the start of the test
- pH adjusted: No
- CEC (meq/100 g): Not specified
- Aeration of dilution water: No
- Suspended solids concentration: Not specified
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 250 to 300 mL BOD bottles with glass stoppers
- Number of culture flasks/concentration: Duplicate
- Method used to create aerobic conditions: Standard O2 in water
- Method used to create anaerobic conditions: Not applicable
- Measuring equipment: Oxygen meter:WTW oxygen meter supplied with a WTW CellOx 325 oxygen electrode, electrolyte type ELY/G.
- Test performed in closed vessels due to significant volatility of test substance: Not applicable although test conditions were closed.
- Test performed in open system: No
SAMPLING
- Sampling frequency: Days 0, 7, 14, 21 and 28.
- Sampling method: Sampled with O2 probe
- Sterility check if applicable: Not applicable
- Sample storage before analysis: O2 measured at specified timepoints
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes (only inoculum, no test item)
- Abiotic sterile control: No
- Toxicity control: Yes (sodium acetate at 2 mg/L, test substance at 1.5 mg/L and inoculum).
- Procedure control: Yes (sodium acetate at 2 mg/L and inoculum)
STATISTICAL METHODS: Not required
Reference substance
- Reference substance:
- acetic acid, sodium salt
Results and discussion
- Test performance:
- 1. Oxygen depletion in the inoculum blank was not significantly higher than 1.5 mg O2/L after 28 days (1.52 mg O2/L).
2. The residual concentration of oxygen in the test bottles was > 0.5 mg/L at any time.
3. Differences between duplicate biodegradation values (expressed as mg O2/L) were less than 20% (1% at the end of the experiment).
4. In the procedure control, sodium acetate was degraded by at least 60% (107%) within 14 days.
Since all criteria for acceptability of the test were met, this study was considered to be valid.
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- >= 62 - <= 67
- Sampling time:
- 28 d
- Details on results:
- The relative biodegradation values calculated from the O2 measurements performed during the test period of 28 days revealed significant biodegradation of the test substance at both concentrations, i.e. 67 % and 62 % for the low and high concentration respectively, based on ThOD. Since the substance is a UVCB it is anticipated that a sequential biodegradation of the individual components takes place. Therefore, the 14-day window will not be applied to interpret the results of this test. In the toxicity control more than 25% biodegradation occurred within 14 days (54%, based on ThOD). Thus, the toxicity control showed that the test substance did not inhibit microbial activity.
BOD5 / COD results
- Results with reference substance:
- In the procedure control, sodium acetate was degraded by at least 60% (107%) within 14 days.
Any other information on results incl. tables
Biodegradation Percentages at Different Points in Time
Test medium |
% Biodegradation after x days1) |
|||||
0 |
7 |
14 |
21 |
28 |
||
Procedure control2) |
A |
0 |
67 |
104 |
||
B |
0 |
59 |
110 |
|||
mean |
0 |
63 |
107 |
|||
Δ |
0 |
8 |
6 |
|||
Test item low3) |
A |
0 |
23 |
47 |
45 |
62 |
B |
0 |
26 |
42 |
62 |
63 |
|
mean |
0 |
25 |
45 |
54 |
63 |
|
Δ |
0 |
3 |
5 |
17 |
1 |
|
Test item high3) |
A |
0 |
26 |
46 |
50 |
56 |
B |
0 |
21 |
45 |
49 |
57 |
|
mean |
0 |
24 |
46 |
50 |
57 |
|
Δ |
0 |
5 |
1 |
1 |
1 |
|
Toxicity control |
A |
0 |
20 |
53 |
||
B |
0 |
30 |
54 |
|||
mean |
0 |
25 |
54 |
|||
Δ |
0 |
10 |
1 |
|||
1): Calculations were performed with the mean oxygen concentrations of t=0 and |
||||||
2): ThOD sodium acetate (reference item): 0.78 mg O2/mg |
||||||
3): ThOD Test substance: 2.49 mg O2/mg |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- In conclusion, the test substance was considered to be readily biodegradable under the conditions of the closed bottle test performed presently.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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